RETRACTED: MicroRNA‐612 inhibits cervical cancer progression by targeting NOB1

Abstract

Recently, many studies have shown that microRNA (miR)‐612 is involved in cancer progression. However, the role of miR‐612 in cervical cancer remains unclear. The present study aims to investigate the biological effects of miR‐612 on cervical cancer. The expression of miR‐612 in cervical cancer tissues and cell lines was analysed by quantitative reverse transcription‐polymerase chain reaction. The effect of miR‐612 cell proliferation, migration, invasion and apoptosis was studied by appropriate methods. Protein expression was determined by Western blot analyses. Bioinformatics analysis and luciferase reporter assays were performed to clarify the relationship between miR‐612 and nin one binding protein (NOB1). A xenograft model was established to examine the role of miR‐612 in vivo tumorigenesis. Cervical cancer tissues and cell lines showed down‐regulation of miR‐612 expression, which was associated with the Fédération Internationale de Gynécologie et d'Obstétrique (FIGO) stage and lymph node metastasis. Functional assays revealed that miR‐612 overexpression significantly suppressed cervical cancer cell proliferation, migration and invasion in vitro and delayed tumour growth in vivo. Mechanically, miR‐612 targeted NOB1 in cervical cancer cells, revealing a negative correlation between miR‐612 and NOB1in cervical cancer samples. NOB1 overexpression partially reversed the inhibitory effects of miR‐612 overexpression in cervical cancer cells. Taken together, these findings indicate that miR‐612 functions as a tumour suppressor in cervical cancer and suggest that miR‐612 may be a potential target in the therapeutic intervention of this malignancy.

<p>Knockdown of MIR4435-2HG Suppresses the Proliferation, Migration and Invasion of Cervical Cancer Cells via Regulating the miR-128-3p/MSI2 Axis in vitro</p>

“…19 MiR-612 has been reported to inhibit the progression of CC. 20 MiR-873 can suppress the viability of CC cells, thus retarding the development of CC. 21 In addition, miR-128 has been reported to play an anti-cancer role in CC.…”

Simultaneous microRNA-612 restoration and 5-FU treatment inhibit the growth and migration of human PANC-1 pancreatic cancer cells

“…In the present experiment, as a first step, the expression of miR-612 in the mentioned cell lines was measured using qRT-PCR following specified cDNA synthesis. Downregulation of miR-612 in the PANC-1 cells after the transfection of its mimic was a promising result due to the downregulation of this very same microRNA in other cancers including HCC, CRC, ovarian and cervical cancers, and melanoma (Sheng et al, 2015[ 20 ]; Yu et al, 2018[ 27 ]; Jin et al, 2020[ 10 ]; Tang et al, 2014[ 24 ]; Zhu et al, 2018[ 28 ]). The findings of this study and previous researches suggest that miR-612 could be a tumor suppressor microRNA in various cancers.…”

“…During recent years, tumor suppressor miRNAs, and microRNA replacement therapy in cancer cells have come into notice (Bader et al, 2010[ 1 ]). miR-612 has shown tumor-suppressive activity in various cancers such as hepatocellular carcinoma, melanoma, bladder, colorectal, ovarian, cervical, and non-small cell lung cancer (Liu et al, 2016[ 15 ]; Sheng et al, 2015[ 20 ]; Liu et al, 2018[ 14 ]; Zhu et al, 2018[ 28 ]; Yu et al, 2018[ 27 ]; Kang et al, 2019[ 11 ]; Jin et al, 2020[ 10 ]).…”

MicroRNAs and Their Influence on the ZEB Family: Mechanistic Aspects and Therapeutic Applications in Cancer Therapy

“…Elevating the expression of miR-506-3p is a potential strategy in ovarian cancer treatment. In the gastric cancer model, it was also observed that a reverse relationship between miR-612 and nin one binding protein (NOB1) helped reduce the migration and invasion of cervical cancer cells [ 73 ]. Similarly, in pancreatic cancer, it was also found that overexpression miRs could lead to better prognosis.…”