MiR-508-5p Inhibits the Progression of Glioma by Targeting Glycoprotein Non-metastatic Melanoma B

Abstract

Glioma is a severe and highly lethal brain cancer, a malignancy largely stemming from growing in a relatively restrained area of the brain. Hence, the understanding of the molecular regulation of the growth of glioma is critical for improving its treatment. MicroRNA has become a hotspot in research on diseases, especially in the initiation and progression of different types of cancer. However, the molecular function and mechanisms of miR-508-5p in gliomagenesis are still unclear. The aim of this study was to investigate miR-508-5p expression in glioma and determine its effects on proliferation. miR-508-5p expression levels, both in glioma cell lines and in tissue, were significantly lower than in a normal human astrocyte cell line or adjacent tissues. Cell growth was analyzed using a MTT assay and over-expression of miR-508-5p was found to decrease glioma cell growth. Moreover, a bioinformatic analysis was performed, showing that glycoprotein non-metastatic melanoma B (GPNMB) was a direct target for miR-508-5p in glioma cells. Furthermore, in vivo treatment with miR-508-5p reduced GPNMB protein levels in the tumor. Additionally, overexpression of GPNMB without 3'-UTR partially reversed the cell growth arrest induced by miR-508-5p over-expression in glioma cells. In conclusion, these results indicate that increased expression of miR-508-5p might be related to glioma progression, indicating a potential role of miR-508-5p for clinical therapy.

YY1‐mediated up‐regulation of lncRNA LINC00466 facilitates glioma progression via miR‐508/CHEK1

“…Previously, many studies have confirmed that miR‐508 played an important role in several tumors, including glioma 38,39 . Importantly, the distinct down‐regulation of miR‐508 and its tumor‐suppressive roles in the proliferation, migration and invasion of glioma cells were also demonstrated in previous studies 38,40 . In the present study, we further performed GO analysis and KEGG assays, finding that miR‐508 is positively associated with the tumor‐related signaling pathway.…”

MicroRNA‐628‐5p inhibits cell proliferation in glioma by targeting DDX59

“…miRNAs, which function as tumor suppressors, play crucial roles in glioma development. [18][19][20] miR-628-5p has been reported in epithelial ovarian cancer and prostate cancer as a tumor suppressor. 10,11 However, miR-628-5p has not been reported before in glioma.…”

LOC102724163 promotes breast cancer cell proliferation and invasion by stimulating MUC19 expression

“…Increasing evidence has confirmed the tumor-repressive role of miR-508-5p. For example, miR-508-5p inhibits the proliferation and migration of glioma cells ( 23 , 35 ). Moreover, miR-508-5p acts as an anti-oncogene by targeting mesoderm development candidate 1 in hepatocellular carcinoma ( 24 ).…”