miR-497 and miR-302b Regulate Ethanol-induced Neuronal Cell Death through BCL2 Protein and Cyclin D2

Abstract

Background: Ethanol-induced neuronal apoptosis causes brain shrinkage and cognitive defects. Results: Exposure to ethanol (0.5% v/v for 72 h) in SH-SY5Y cells induced expression of miR-497 and miR-302b and downregulated expression of BCL2 and/or cyclin D2. Conclusion: Ethanol-induced neuronal apoptosis follows both the mitochondria-mediated and non-mitochondria-mediated pathways. Significance: Our study shows that miRNAs are involved in regulation of ethanol neurotoxicity.

MicroRNA-29b Regulates Ethanol-induced Neuronal Apoptosis in the Developing Cerebellum through SP1/RAX/PKR Cascade

“…Using SH-SY5Y cells, a human neuroblastoma cell line, Yadav et al (39) reported that ethanol induced cell apoptosis by up-regulation of miR-497 and miR-302b, which inhibited the expression of their target genes, Bcl-2 and cyclin D2. Sathyan et al (5) used a model of fetal mouse cerebral cortexderived neurosphere (the neurons were isolated from the cerebra at gestational day 12.5) to show that ethanol at 320 mg/dl suppressed expression of four miRNAs: miR-21, -335, -9, and -153.…”

“…MiRNAs have been shown to regulate diverse cellular functions including cell survival/death. When we started the study, we performed a literature search and found 26 miRNAs that have been reported to mediate survival/ apoptosis in neuronal and glia cells (5,11,(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39) (Fig. 1B).…”

MicroRNA-497 Suppresses Proliferation and Induces Apoptosis in Prostate Cancer Cells

“…Up to now, more than 50 miRNAs are abnormally expressed, leading to alteration in the expression and activity of their targets in PCa, such as miR-21, miR-320, miR-29b and miR34c (Ribas et al, 2009;Hagman et al, 2010;Hsieh et al, 2012;Ru et al, 2012 all prostate carcinoma samples compared with the BPH samples (Porkka et al, 2007;Watahiki et al, 2011). Several studies showed that the potential functions of miR-497 in human breast cancer (Li et al, 2011;Shen et al, 2012), neuroblastoma (Yadav et al, 2011), cervical cancer (Zheng et al, 2012), gastric cancer (Zhu et al, 2012) and colorectal cancer (Guo et al, 2012). However, no studies about the role of miRNA-497 in prostate cancer have been reported.…”

microRNAs in Cancer Cell Response to Ionizing Radiation

“…Not surprisingly, many of the miRNAs that are capable of targeting MCL1 and/or Bcl-2 have already been discussed as regulators of other DDR pathway members. In addition to miR-181a, mentioned earlier, miR-7, miR-34a, miR-125b, miR-143, miR-155, and members of the miR-15/16 family, including miR-15b, miR-16, miR-195, and miR-497, target Bcl-2 as well as other DDR proteins (57,58,85,148,153,154,156,166,172). In fact, one key report found that a frequent deletion of chromosome 13q14 seen in chronic lymphocytic leukemia (CLL) contained the miR-15 and miR-16 loci, resulting in repression of miR-15 and miR-16 expression (10).…”