miR-486 functions as a tumor suppressor in esophageal cancer by targeting CDK4/BCAS2

Lang, Baoping; Zhao, Song

doi:10.3892/or.2017.6064

Cited by 34 publications

(29 citation statements)

References 47 publications

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“…MiR-486 has been shown to interfere with gastric cancer cell apoptosis by regulating PTEN and PIM-1 [16,17], and elevated expression may have a more general antiapoptotic effect as it is associated with reduced expression of the tumor suppressor p53 and the apoptosis effector caspase-3 [18]. Conversely, miR-486 was found to suppress the growth and metastasis of esophageal cancer by targeting CDK4/BCAS2 [19]. Low miR-486 expression has been observed in esophageal squamous cell carcinoma and gastric adenocarcinoma, suggesting utility as an independent marker to evaluate prognosis [20][21][22].…”

Section: Discussionmentioning

confidence: 99%

MiR-486-5p Suppresses Proliferation and Migration of Hepatocellular Carcinoma Cells through Downregulation of the E3 Ubiquitin Ligase CBL

Xiao

et al. 2019

BioMed Research International

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MicroRNAs have been broadly implicated in cancer, but precise functions and mechanisms in carcinogenesis vary among cancer types and in many cases remain poorly understood. Hepatocellular carcinoma (HCC) is among the most frequent and lethal cancers. The aim of the present study was to investigate the role of miR-486-5p in HCC and identify its specific target. MiR-486-5p was significantly downregulated in HCC tissues and cell lines compared with noncancerous tissues and, respectively, although expression level was not correlated with the degree of infiltration or tumor stage. However, miR-486-5p overexpression in HCC cells inhibited proliferation and migration as evidenced by CCK-8 cell counting, wound healing, and transwell assays, indicating that miR-486-5p is an HCC suppressor. We employed four miRNA databases to predict the target genes of miR-486-5p and verified retrieved genes using qPCR and western blotting. The E3 ubiquitin ligase CBL was significantly downregulated by miR-486-5p overexpression in HCC cell lines at both mRNA and protein level, and overexpression of CBL counteracted the inhibitory effects of miR-486-5p on HCC cell proliferation and migration. Moreover, CBL expression was negatively correlated with miR-486-5p expression in HCC tissues. Collectively, our results suggest that miR-486-5p may act as a tumor suppressor gene in HCC by downregulating CBL expression.

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Section: Discussionmentioning

confidence: 99%

MiR-486-5p Suppresses Proliferation and Migration of Hepatocellular Carcinoma Cells through Downregulation of the E3 Ubiquitin Ligase CBL

Xiao

et al. 2019

BioMed Research International

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“…It is demonstrated that the biological function of miRNAs is generally achieved by regulating target genes by binding the 3′-untranslated region (3′-UTR) of the target messenger RNA (mRNA) [10]. Accumulated evidence shown that the miRNAs can be involved in the regulation of tumor cell biological processes by targeting oncogenes or tumor suppressors [11][12][13]. Therefore, to identify functional miR-NAs that abnormally expressed in NSCLC may provide novel therapeutic ideas for cancer treatment.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-1298 is downregulated in non-small cell lung cancer and suppresses tumor progression in tumor cells

Wang

et al. 2019

Diagn Pathol

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Background: MicroRNAs (miRNAs) have been reported to serve pivotal roles in tumorigenesis. This study sough to assess the expression and clinical significance of microRNA-1298 (miR-1298) in patients with non-small cell lung cancer (NSCLC), and explore the functional role of miR-1298 in tumorigenesis. Methods: One hundred and twenty-one NSCLC patients were recruited in this study. The expression of miR-1298 was estimated using quantitative real-time PCR. Kaplan-Meier survival curves and Cox regression analysis were used to evaluate the prognostic value of miR-1298. Gain-and loss-of-function experiments were preformed to explore the biological function of miR-1298 in NSCLC cells. Results: Expression levels of miR-1298 were downregulated in NSCLC tissues and cells compared with the corresponding normal controls. The decreased expression of miR-1298 was associated with patients' lymph node metastasis and TNM stage. The low expression of miR-1298 predicted poor overall survival and served as an independent prognostic indicator in NSCLC patients. According to the cell experiments, NSCLC cell proliferation, migration and invasion were inhibited by the overexpression of miR-1298. Conclusion: All the data indicated that the downregulation of miR-1298 predicts poor prognosis of NSCLC, and the overexpression of miR-1298 in NSCLC cells leads to inhibited tumorigenesis. The aberrant miR-1298 may serve as a novel biomarker and therapeutic target in NSCLC.

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“…ESCA is one of the most common tumors and a notable threat to human health ( 18 ). Despite traditional therapies having improved greatly, the prognosis and 5-year survival rates of patients with ESCAs remain poor ( 19 ).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑454‑3p inhibits cell proliferation and invasion in esophageal cancer by targeting insulin‑like growth factor 2 mRNA‑binding protein 1

et al. 2020

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Esophageal cancer (ESCA) is the eighth most common cause of cancer-associated mortality in humans. An increasing number of studies have demonstrated that microRNAs (miRs) serve important roles in mediating tumor initiation and progression. miR-454-3p has been found to be involved in the development of various human malignancies; however, little is known about the role of miR-454-3p in esophageal cancer. In the present study, the protein and gene expression levels of miR-454-3p in ESCA tissues and cells were downregulated compared with adjacent normal tissues and normal human esophageal epithelial cells. Additionally, miR-454-3p downregulation resulted in improved survival rates in patients with ESCA, and miR-454-3p overexpression significantly suppressed cell proliferation, migration and invasion and promoted apoptosis in four ESCA cell lines (EC9706, ECA109, TE-1 and TE-8). It was found that miR-454-3p overexpression inhibited the expression of insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) at the protein and mRNA expression levels. Furthermore, it was demonstrated that miR-454-3p inhibited ESCA cell proliferation, migration and apoptosis by targeting IGF2BP1 via the ERK and AKT signaling pathways in a subcutaneous xenograft tumor mouse model. These results showed that miR-454-3p functioned as an important tumor suppressor in ESCA by targeting IGFBP1. Therefore, miR-454-3p may be a novel prognostic biomarker and therapeutic target for patients with ESCA.

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miR-486 functions as a tumor suppressor in esophageal cancer by targeting CDK4/BCAS2

Cited by 34 publications

References 47 publications

MiR-486-5p Suppresses Proliferation and Migration of Hepatocellular Carcinoma Cells through Downregulation of the E3 Ubiquitin Ligase CBL

MiR-486-5p Suppresses Proliferation and Migration of Hepatocellular Carcinoma Cells through Downregulation of the E3 Ubiquitin Ligase CBL

MicroRNA-1298 is downregulated in non-small cell lung cancer and suppresses tumor progression in tumor cells

MicroRNA‑454‑3p inhibits cell proliferation and invasion in esophageal cancer by targeting insulin‑like growth factor 2 mRNA‑binding protein 1

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