miR-4461 Regulates the Proliferation and Metastasis of Ovarian Cancer Cells and Cisplatin Resistance

Abstract

microRNAs (miRNAs) are of great significance in cancer treatment, which may have a desirable result on the regulation of tumorigenesis, progression, recurrence, and chemo-resistance of ovarian cancer. However, the research on the further potential application of miR-4461 in ovarian cancer is little and limited. Therefore, the study in this paper focus on the investigation of the of miR-4461 in ovarian cancer progression and chemo-resistance. The phenomenon that the proliferation and metastasis of ovarian cancer cells can be promoted by miR4461 is revealed in functional assays. Through the bioinformatics and luciferase reporter analysis, the PTEN is validated to be the direct target of miR-4461 in ovarian. The association between the expression of miR-4461 and PTEN is negative in in human ovarian cancer tissues. The distinction of growth and metastasis capacity between miR-4461 knockdown ovarian cancer cells and control cells is partially abolished by si-PTEN. Moreover, it was found that cisplatin treatment has obvious effect on the miR-4461 knockdown ovarian cancer cells. In summary, the data given in this paper indicate that the miR-4461 can be regarded as a potential onco-miRNA in ovarian cancer by targeting PTEN.

Alkannin Inhibits the Development of Ovarian Cancer by Affecting miR-4461

“…More importantly, alkannin induced apoptosis and inhibited the migration of OC cells by inhibiting the phosphorylation of Src and FAK [ 29 ], which is consistent with our results. Different from the above studies, we found that alkannin plays an antitumor effect in OC by downregulating miR-4461 [ 18 ]. Moreover, miR-4461 can promote cell growth and metastasis in OC.…”

“…miR-4461 has been reported to inhibit the tumorigenesis of renal cell carcinoma by targeting PPP1R3C [ 17 ]. However, miR-4461 was regarded as a potential onco-miRNA in OC by targeting PTEN [ 18 ]. These results indicate that miR-4461 is tissue specific.…”

miR-600 promotes ovarian cancer cells stemness, proliferation and metastasis via targeting KLF9

“…For instance, miR-522 has been identified to promotes ovarian cancer progression by regulating PTEN pathway [ 21 ]. miR-4461 has been revealed to promotes the proliferation and metastasis of ovarian cancer cells and cisplatin resistance via targeting PTEN [ 22 ]. miR-600 is a newly discovered miRNA, and its role and mechanism in biological development and disease are not completely understood.…”

Cisplatin for cancer therapy and overcoming chemoresistance

“…Furthermore, miR 4461 induces chemoresistance to cisplatin therapy in OC [ 152 ]. It augments in vitro OC cell proliferation and metastases where the knockdown of it in OC produced sensitivity to the drug via the stimulation of lipid and protein phosphatase and tensin homolog (PTEN), thereby suppressing cancer progression [ 152 ].…”

“…Cellular miR 409-3p overexpression augments sensitivity to cisplatin by conjugation of miR-409-3p and 3 0 -UTR in FIP 200 mRNA and hence, enhance FIP 200-mediated autophagy to alleviate chemoresistance in OC [151]. Furthermore, miR 4461 induces chemoresistance to cisplatin therapy in OC [152]. It augments in vitro OC cell proliferation and metastases where the knockdown of it in OC produced sensitivity to the drug via the stimulation of lipid and protein phosphatase and tensin homolog (PTEN), thereby suppressing cancer progression [152].…”