2017
DOI: 10.3892/etm.2017.5444
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miR-4262 regulates chondrocyte viability, apoptosis, autophagy by targeting SIRT1 and activating PI3K/AKT/mTOR signaling pathway in rats with osteoarthritis

Abstract: Abstract. The present study aimed to investigate the effect and underlying mechanism of microRNA (miR)-4262 in the development of osteoarthritis (OA) in rats. Primary chondrocytes were separated from Sprague-Dawley rats and then treated with tumor necrosis factor-α (TNF-α). The level of miR-4262 was detected in TNF-α-treated chondrocytes, and then the miR-4262 or its target gene sirtuin type 1 (SIRT1) level was overexpressed, or knocked down. Furthermore, cell viability, cell apoptosis, cell autophagy and matr… Show more

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Cited by 33 publications
(32 citation statements)
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References 39 publications
(37 reference statements)
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“…Akt signaling has been proven to be involved in the pathogenesis of osteoarthritis [ 11 ]. In our study, lncRNA-ATB overexpression showed no significant effect on the expression level of Akt in the cells of human chondrocyte cell line CHON-001 (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Akt signaling has been proven to be involved in the pathogenesis of osteoarthritis [ 11 ]. In our study, lncRNA-ATB overexpression showed no significant effect on the expression level of Akt in the cells of human chondrocyte cell line CHON-001 (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…This miR is induced by IL-1β in rat chondrocytes and promotes apoptosis, induces iNOS expression, and decreases Col2a1 expression (85). miR-4262, which targets SIRT1, is increased in TNF-α-treated rat chondrocytes and promotes chondrocyte apoptosis and inhibits autophagy, contributing to OA pathogenesis (86). Loss of matrix synthesis proteins and elevation of matrix-degrading enzymes, such as MMP-13 and ADAMTS-5, was also observed downstream of miR-4262 (86).…”
Section: Mirs Involved In Cartilage-destructive Mechanismsmentioning
confidence: 99%
“…In primary human and TC28a2 chondrocytes, miR-155 inhibited autophagy and contributed to the autophagy defects in OA (D'Adamo et al, 2016 ). In rats with OA, miR-4262 regulates chondrocyte fate by influencing PI3K/AKT/mTOR signaling (Sun et al, 2018 ). In OA chondrocytes and in zebra fish, Fis1 suppression induces accumulation and inhibition of lysosomes by altering miRNAs and energy signals (Kim et al, 2016 ).…”
Section: Autophagy In Oamentioning
confidence: 99%