miR-382 inhibits tumor progression by targeting SETD8 in non-small cell lung cancer

Chen, Tianjun; Ren, Hui; Thakur, Asmitananda; Tian, Yang; Li, Yang; Zhang, Shuo; Wang, Ting; Chen, Mingwei

doi:10.1016/j.biopha.2016.12.007

Cited by 37 publications

(34 citation statements)

References 29 publications

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“…For instance, miR-382 was significantly downregulated in non-small cell lung cancer. Additionally, low miR-382 expression level was correlated with the tumour stage and metastasis of non-small cell lung cancer (29). The miR-382 expression level was decreased in highly metastatic osteosarcoma cell lines and relapsed osteosarcoma tissues.…”

Section: Discussionmentioning

confidence: 93%

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MicroRNA-382 inhibits cell proliferation and invasion of retinoblastoma by targeting BDNF-mediated PI3K/AKT signalling pathway

Song

Diao

Yang

et al. 2017

Molecular Medicine Reports

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It has previously been demonstrated that multiple microRNAs (miRNAs or miRs) are aberrantly expressed in retinoblastoma (RB) and contribute to RB initiation and progression. miR‑382 has been revealed to be aberrantly expressed and therefore exhibits a key role in the progression of various types of cancer. However, the expression pattern, functional roles and underlying molecular mechanism of miR‑382 in RB remain unknown. The present study investigated the expression levels of miR‑382 and its effects on RB cells and the underlying regulatory mechanism of its action. It was demonstrated that miR‑382 was downregulated in RB tissues and cell lines. Upregulation of miR‑382 inhibited RB cell proliferation and invasion in vitro. Additionally, brain‑derived neurotrophic factor (BDNF) was identified as a novel target of miR‑382 in RB. BDNF was upregulated in RB tissues and negatively associated with miR‑382 expression levels. Furthermore, BDNF overexpression rescued the tumour‑suppressing effects on RB cells induced by miR‑382. miR‑382 inactivated the phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) signalling pathway in RB. These findings suggested that miR‑382 serves as a tumour suppressor in RB, in part, by targeting the BDNF‑mediated PI3K/AKT signalling pathway. The results of the present study suggest a potential therapeutic strategy for treating RB patients in the future.

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Section: Discussionmentioning

confidence: 93%

“…miR-382 is aberrantly expressed and play important roles in several types of cancer (29)(30)(31). However, the expression pattern, functional roles and underlying molecular mechanism of miR-382 in RB remains unknown.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-382 inhibits cell proliferation and invasion of retinoblastoma by targeting BDNF-mediated PI3K/AKT signalling pathway

Song

Diao

Yang

et al. 2017

Molecular Medicine Reports

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“…Previous studies have indicated that multiple members from miR‐382 family were implicated in the inhibition of malignancies, such as colorectal cancer, liver cancer, and esophageal squamous cell carcinoma . From a mechanistic point of view, miR‐382 impedes the progression of NSCLC by repressing SETD8 expression . MiR‐382 arrested the metastasis of prostate cancer cells via modulating COUP‐TFII .…”

Section: Discussionmentioning

confidence: 99%

Circ_0001658 promotes the proliferation and metastasis of osteosarcoma cells via regulating miR‐382‐5p/YB‐1 axis

Wang

et al. 2019

Cell Biochemistry & Function

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Circular RNA (circRNA) can modulate the gene expression via sponging microRNA (miRNA) in multiple malignancies. Nevertheless, the detailed function of circ_0001658 in osteosarcoma (OS) and its regulatory mechanism remain elusive.Real-time PCR was carried out to detect the expressions of circ_0001658, miR-382-5p, and Y box-binding protein 1 (YB-1) mRNA in OS tissues. Besides, western blot was done to monitor YB-1 expression in OS cells. Chi-squared test was used to analyse the correlation between circ_0001658 and clinicopathologic characteristics of OS patients. CCK8 assay, colony formation assay, flow cytometry, and transwell assay were performed to determine the function of circ_0001658. Moreover, dualluciferase reporter gene assay was done to verify the targeting relationship between circ_0001658 and miR-382-5p. Compared with adjacent tissues, circ_0001658 displayed a significantly higher expression in OS tissues. Circ_0001658 could facilitate the proliferation, migration, and invasion of OS cells and impede apoptosis by sponging miR-382-5p. Further, circ_0001658 was proved to directly and negatively regulate the expression of miR-382-5 while positively modulate the expression of YB-1. Circ_0001658 promotes the proliferation and metastasis of OS cells via modulating miR-382-5p/YB-1 axis. Significance of the study:In recent years, the expression and function of circular RNA in cancer have been the focus of tumour research. The study on circular RNA helps elucidate the molecular pathology of tumorigenesis and cancer progression.This study demonstrated that circ_0001658 promotes the proliferation and metastasis of OS cells via modulating miR-382-5p/YB-1 axis. To our best knowledge, this work is the first to study the expression and function of circ_0001658 in cancer. K E Y W O R D Scirc_0001658, miR-382-5p, osteosarcoma, YB-1 | INTRODUCTIONOsteosarcoma (OS) is a common primary malignant bone tumour among children and adolescents, frequently diagnosed in the distal femur, the proximal tibia, and the proximal humerus. 1,2 Though multiple treatments offer symptomatic relief and modest improvement in survival for OS patients, the long-term disease-free survival rate of patients remains unfavourable, only 60% to 75%. 3,4 To make matters worse, the 5-year survival rate was only 27% to 55% in patients with local relapsing and distant metastasis treated by the available therapy clinically. 5 Hence, it is imperative to probe into the detailed mechanism of OS.Circular RNA (circRNA) is a novel class of endogenous noncoding RNA, which exists widely and stably in mammalian cells without the function of encoding proteins. 6,7 Circ-RNA, unlike linear RNAs, features a closed-loop structure. 8 This distinctive structure contributes

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“…Chen et al, 2019). Besides, miR-382 was significantly downregulated in NSCLC tissues and cells, and enrichment of miR-382 depleted cell proliferation, migration and invasion through targeting SETD8 (Chen et al, 2017). Consistent with these findings, we also detected that miR-382-5p was weakly expressed in NSCLC cells, and reintroduction of miR-382-5p inhibited proliferation, autophagy and glycolysis of NSCLC cells.…”

Section: Discussionmentioning

confidence: 99%

Circ_0005962 functions as an oncogene to aggravate non-small cell lung cancer progression via circ_0005962/miR-382-5p/PDK4 regulatory network

Zhenxiu¹,

Chen²,

Peng³

et al. 2020

Preprint

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Non-small cell lung cancer (NSCLC) is a leading threat to human lives with high incidence and mortality. Circular RNAs (circRNAs) were reported to play important roles in human cancers. The purpose of this study was to investigate the role of circ_0005962 and explore the underlying functional mechanisms. The expression of circ_0005962, miR-382-5p and pyruvate dehydrogenase kinase 4 (PDK4) was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation and cell apoptosis were assessed by cell counting kit-8 (CCK-8) assay and flow cytometry assay, respectively. The protein levels of Beclin 1, light chain3 (LC3-II/LC3-I), PDK4, Cleaved Caspase 3 (C-caspase 3) and proliferating cell nuclear antigen (PCNA) were examined using western blot analysis. Glycolysis was determined according to the levels of glucose consumption and lactate production. The interaction between miR-382-5p and circ_0005962 or PDK4 was predicted by the online tool CircInteractome or starbase and verified by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. Xenograft model was constructed to investigate the role of circ_0005962 in vivo. circ_0005962 expressed with a high level in NSCLC tissues and cells. Circ_0005962 knockdown inhibited proliferation, autophagy, and glycolysis but promoted apoptosis in NSCLC cells. MiR-382-5p was targeted by circ_0005962, and its inhibition reversed the role of circ_0005962 knockdown. Besides, PDK4, a target of miR-382-5p, was regulated by circ_0005962 through miR-382-5p, and its overexpression abolished the effects of miR-382-5p reintroduction. Circ_0005962 knockdown suppressed tumor growth in vivo. Circ_0005962 knockdown restrained cell proliferation, autophagy, and glycolysis but stimulated apoptosis through modulating the circ_0005962/miR-382-5p/PDK4 axis. Our study broadened the insights into understanding the mechanism of NSCLC progression.

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miR-382 inhibits tumor progression by targeting SETD8 in non-small cell lung cancer

Cited by 37 publications

References 29 publications

MicroRNA-382 inhibits cell proliferation and invasion of retinoblastoma by targeting BDNF-mediated PI3K/AKT signalling pathway

MicroRNA-382 inhibits cell proliferation and invasion of retinoblastoma by targeting BDNF-mediated PI3K/AKT signalling pathway

Circ_0001658 promotes the proliferation and metastasis of osteosarcoma cells via regulating miR‐382‐5p/YB‐1 axis

Circ_0005962 functions as an oncogene to aggravate non-small cell lung cancer progression via circ_0005962/miR-382-5p/PDK4 regulatory network

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