miR-381-abundant small extracellular vesicles derived from kartogenin-preconditioned mesenchymal stem cells promote chondrogenesis of MSCs by targeting TAOK1

Jing, Hui; Zhang, Xiaoyang; Luo, Kai; Luo, Qiancheng; Yin, Meng; Wang, Wei; Zhu, Zhenan; Zheng, Jie; He, Xiaomin

doi:10.1016/j.biomaterials.2019.119682

Cited by 52 publications

(57 citation statements)

References 41 publications

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“…A recent study found that EVs derived from human WJMSCs pretreated with KGN contain a unique miRNA, miR-381-3p. Researchers found that miR-381-3p directly inhibited TAOK1 by targeting the 3′ untranslated region of TAOK1, thus inhibiting the Hippo signaling pathway and mediating cartilage formation [ 120 ].…”

Section: Stem Cell Pretreatment Strategymentioning

confidence: 99%

Research Progress on Stem Cell Therapies for Articular Cartilage Regeneration

Jiang

Tian

et al. 2021

Stem Cells International

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Injury of articular cartilage can cause osteoarthritis and seriously affect the physical and mental health of patients. Unfortunately, current surgical treatment techniques that are commonly used in the clinic cannot regenerate articular cartilage. Regenerative medicine involving stem cells has entered a new stage and is considered the most promising way to regenerate articular cartilage. In terms of theories on the mechanism, it was thought that stem cell-mediated articular cartilage regeneration was achieved through the directional differentiation of stem cells into chondrocytes. However, recent evidence has shown that the stem cell secretome plays an important role in biological processes such as the immune response, inflammation regulation, and drug delivery. At the same time, the stem cell secretome can effectively mediate the process of tissue regeneration. This new theory has attributed the therapeutic effect of stem cells to their paracrine effects. The application of stem cells is not limited to exogenous stem cell transplantation. Endogenous stem cell homing and in situ regeneration strategies have received extensive attention. The application of stem cell derivatives, such as conditioned media, extracellular vesicles, and extracellular matrix, is an extension of stem cell paracrine theory. On the other hand, stem cell pretreatment strategies have also shown promising therapeutic effects. This article will systematically review the latest developments in these areas, summarize challenges in articular cartilage regeneration strategies involving stem cells, and describe prospects for future development.

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Section: Stem Cell Pretreatment Strategymentioning

confidence: 99%

Research Progress on Stem Cell Therapies for Articular Cartilage Regeneration

Jiang

Tian

et al. 2021

Stem Cells International

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“…MicroRNA miR-706 directly inhibits PKCα and TAOK1 expression via binding to their 3′-untranslated regions, thus preventing the epithelial to mesenchymal transition and alleviating hepatic fibrosis [ 68 ]. Jing et al [ 69 ] showed that miR-381-3p promoted chondrogenesis in umbilical cord mesenchymal stem cells through direct suppression of TAOK1 and the downstream Hippo signaling pathway, indicating that TAOK1 may regulate chondrogenesis via the Hippo cascade. In an acoustic trauma study by Patel et al [ 70 ], the downregulation of miRNA-183 and upregulation of TAOK1 were observed in noise-traumatized cochlear cells in rats, indicating that the miR-183/TAOK1 pathway is likely to play a role in sensorineural hearing loss.…”

Section: Tao Kinases In Other Physiological Processes and Diseasesmentioning

confidence: 99%

The Diverse Roles of TAO Kinases in Health and Diseases

Fang

Lai

Hsiao

et al. 2020

IJMS

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Thousand and one kinases (TAOKs) are members of the MAP kinase kinase kinase (MAP3K) family. Three members of this subfamily, TAOK1, 2, and 3, have been identified in mammals. It has been shown that TAOK1, 2 and 3 regulate the p38 MAPK and Hippo signaling pathways, while TAOK 1 and 2 modulate the SAPK/JNK cascade. Furthermore, TAOKs are involved in additional interactions with other cellular proteins and all of these pathways modulate vital physiological and pathophysiological responses in cells and tissues. Dysregulation of TAOK-related pathways is implicated in the development of diseases including inflammatory and immune disorders, cancer and drug resistance, and autism and Alzheimer’s diseases. This review collates current knowledge concerning the roles of TAOKs in protein–protein interaction, signal transduction, physiological regulation, and pathogenesis and summarizes the recent development of TAOK-specific inhibitors that have the potential to ameliorate TAOKs’ effects in pathological situations.

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“…Although many studies show that miR-381 regulates several key biological functions, including chondrogenesis [29], cell proliferation [30], tumor suppression [31,32], inflammation [33], and neuromodulation [34], there have been no reports to date implicating miR-381 in adipogenesis. In our previous miRNA-seq and RNA-seq analyses, we found that miR-381 was differentially expressed in the longest dorsal muscles of Yanbian yellow cattle in the high-and low-fat groups, and that miR-381 was within the upper-middle range of endogenous expression among differentially expressed miRNAs.…”

Section: Discussionmentioning

confidence: 99%

miR-381 Targets KCTD15 to Regulate Bovine Preadipocyte Differentiation In Vitro

Shao

Fang

et al. 2020

Horm Metab Res

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MicroRNAs (miRNAs) are small, single-stranded, noncoding RNAs ~21 to ~23 nucleotides in length and have become a popular research topic in recent years due to their regulation of gene expression and many physiological processes, including fat metabolism; however, the precise functional mechanisms underlying their regulation of fat metabolism are not fully understood. Here, we identified miR-381, which specifically targets the 3′ untranslated region (3′ UTR) of potassium channel tetramerization-domain-containing 15 (KCTD15) , and verified the mechanism regulating its expression and participation in adipogenesis. We used a dual luciferase-reporter assay and transfection-mediated miR-381 overexpression and inhibition in Yanbian yellow cattle preadipocytes to investigate the role of miR-381 in adipogenesis. The results showed that miR-381 directly targets the 3′ UTR of KCTD15 and downregulates its expression. Additionally, miR-381 overexpression using an miRNA mimic promoted triglyceride accumulation and upregulated adipogenic peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT enhancer-binding protein α (C/EBPα) at both the protein and mRNA levels, whereas miR-381 inhibition produced the opposite effect. These results indicated that miR-381 regulates the differentiation of Yanbian yellow cattle preadipocytes by inhibiting KCTD15 expression, thereby highlighting the importance of miRNA-mediated regulation of adipogenesis. Furthermore, our findings suggested that miR-381 and its target gene(s) might represent new targets for investigating intramuscular fat deposits in cattle and treating human obesity.

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miR-381-abundant small extracellular vesicles derived from kartogenin-preconditioned mesenchymal stem cells promote chondrogenesis of MSCs by targeting TAOK1

Cited by 52 publications

References 41 publications

Research Progress on Stem Cell Therapies for Articular Cartilage Regeneration

Research Progress on Stem Cell Therapies for Articular Cartilage Regeneration

The Diverse Roles of TAO Kinases in Health and Diseases

miR-381 Targets KCTD15 to Regulate Bovine Preadipocyte Differentiation In Vitro

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