MiR-378a-5p Regulates Proliferation and Migration in Vascular Smooth Muscle Cell by Targeting CDK1

Liu, Shaoyan; Yang, Yanyan; Jiang, Shaoyan; Xu, Hong; Tang, Ningning; Lobo, Amara; Zhang, Rui; Liu, Song; Yu, Tao; Hui, Xin

doi:10.3389/fgene.2019.00022

Cited by 45 publications

(37 citation statements)

References 31 publications

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“…The scheme reported in Supplementary Fig. 7 depicts: (a) STAMBP and HOXD10 as new miR-378a-5p target genes, in addition to the previously reported ones 14,16,20,23,[43][44][45][46][47][48][49] ; (b) miR-378a-5p regulation by Bcl-2 identified beyond the already demonstrated players affecting miR-378a-5p expression 28,43,50 and biologic effects observed in melanoma. This study sheds light on the possibility of targeting oncogenic mi-R378a-5p for melanoma therapy.…”

Section: Discussionmentioning

confidence: 89%

microRNA-378a-5p iS a novel positive regulator of melanoma progression

et al. 2020

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Evaluating the expression levels of miR-378a-5p both in a large melanoma patient cohort from The Cancer Genome Atlas database and in melanoma patients from our Institute, we found that miR-378a-5p is upregulated in metastatic melanoma specimens. miR-378a-5p expression was also increased in melanoma cells resistant to target therapy, and decreased in response to drug treatment. We also demonstrated that overexpression of miR-378a-5p enhances in vitro cell invasion and migration, and facilitates the ability of melanoma cells to form de novo vasculogenic structures. While performing downstream targeting studies, we confirmed the ability of miR-378a-5p to modulate the expression of known target genes, such as SUFU, FUS-1, and KLF9. Luciferase-3′UTR experiments also identified STAMBP and HOXD10 as new miR-378a-5p target genes. MMP2 and uPAR, two HOXD10 target genes, were positively regulated by miR-378a-5p. Genetic and pharmacologic approaches inhibiting uPAR expression and activity evidenced that the in vitro tumor-promoting functions of miR-378a-5p, were in part mediated by uPAR. Of note miR-378a-5p was also able to increase VEGF, as well as in vitro and in vivo angiogenesis. Finally, genetic and pharmacologic modulation of Bcl-2 evidenced Bcl-2 ability to regulate miR-378a-5p expression. In conclusion, to the best of our knowledge, this is the first study demonstrating that miR-378a-5p acts as an oncogenic microRNA in melanoma.

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Section: Discussionmentioning

confidence: 89%

microRNA-378a-5p iS a novel positive regulator of melanoma progression

et al. 2020

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“…Proliferation, migration and phenotypic transformation of SMcs or endothelial cells are associated with various miRNAs (48) including miR-145 (49,50), miR-222 (51), miR-195 (52) and miR-21 (53). For example, Liu et al (54) confirmed that the upregulation of miR-378a targeted cyclin-dependent kinase 1 to promote the proliferation and migration of vascular SMcs and increase the incidence of in-stent restenosis following stenting. Huang et al (55) suggested that miR-22-3p overexpression inhibited proliferation and migration of human artery vascular SMcs and prevented neointimal hyperplasia by targeting high mobility group box-1.…”

Section: Discussionmentioning

confidence: 99%

Upregulation of miR‑423 improves autologous vein graft restenosis via targeting ADAMTS‑7

Ren

Liang

et al. 2019

Int J Mol Med

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coronary artery bypass graft (cABG) is one of the primary methods of treating coronary heart disease (cHd); however, vein graft restenosis is a major limiting factor of the effectiveness of cABG. Emerging evidence has indicated that miR-423 is associated with vascular diseases. Additionally, upregulation of a disintegrin and metalloproteinase with thrombospondin motifs-7 (AdAMTS-7) contributes to neointima formation by promoting the proliferation and migration of vascular smooth muscle cells and inhibiting the proliferation and migration of endothelial cells. The aim of the present study was to examine the effects of miR-423 target, AdAMTS-7, on regulating vein graft disease and identify novel biomarkers for use in therapy of vein graft failure (VGF). Aberrant expression of miR-423 in plasma of patients with CHD prior to and following CABG confirms that miR-423 may be a suitable target for preventing VGF. Furthermore, a dual-luciferase reporter gene assay indicated that miR-423 directly interacted with AdAMTS-7 and suppressed its expression. Ectopic expression of miR-423 suppressed AdAMTS-7, resulting in decreased proliferation and migration rates of human umbilical vein smooth muscle cells by targeting AdAMTS-7, but resulted in increased proliferation and migration of human umbilical vein endothelial cells in vitro. Overexpression of miR-423 also enhanced re-endothelialization and decreased neointimal formation in a rat vein graft model. In conclusion, the results of the present study demonstrated that the miR-423/AdAMTS-7 axis may possess potential clinical value for the prevention and treatment of restenosis in patients with cHd following cABG.

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“…In addition, abnormal proliferation or migration of VSMCs can lead to vessel lesions, resulting in AS and in stent-restenosis. e expression of miR-378a-5p was increased in the group with stent restenosis compared with healthy people, and miR-378a-5p overexpression promoted proliferation and migration in VSMCs by targeting CDK1 [26]. e changes in VSMC phenotype and vascular calcification are major characteristics of AS.…”

Section: Regulation Of Mirnas In Chdmentioning

confidence: 99%

Advances in Research on the circRNA-miRNA-mRNA Network in Coronary Heart Disease Treated with Traditional Chinese Medicine

Lin

Chen

Zhao

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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There has been an increase in morbidity and mortality related to coronary heart disease (CHD) in China in recent years. Numerous clinical experiences and studies have shown that traditional Chinese medicine (TCM) plays an important role in the prevention, treatment, and prognosis of CHD. However, the mechanism of TCM in the treatment of CHD has not yet been elucidated. The circRNA-miRNA-mRNA network consists of miRNA that is competitively bound by circRNA, and miRNA regulates the transcription level of mRNA. Through literature review, we found that the circRNA-miRNA-mRNA network acts to contribute to certain effects to CHD such as myocardial hypertrophy, myocardial fibrosis, and heart failure. TCM contains constituents that act against CHD by antiatherosclerosis and apoptosis inhibition action, cardiac and cardiomyocyte protection, and these components also promote cell growth and protection of the vascular system by regulating miRNAs. Therefore, we consider that the circRNA-miRNA-mRNA network may be a new regulatory mechanism for the effective treatment of CHD by TCM.

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MiR-378a-5p Regulates Proliferation and Migration in Vascular Smooth Muscle Cell by Targeting CDK1

Cited by 45 publications

References 31 publications

microRNA-378a-5p iS a novel positive regulator of melanoma progression

microRNA-378a-5p iS a novel positive regulator of melanoma progression

Upregulation of miR‑423 improves autologous vein graft restenosis via targeting ADAMTS‑7

Advances in Research on the circRNA-miRNA-mRNA Network in Coronary Heart Disease Treated with Traditional Chinese Medicine

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