miR‐362‐5p inhibits proliferation and migration of neuroblastoma cells by targeting phosphatidylinositol 3‐kinase‐C2β

Wu, Kai; Lü, Yang; Chen, Jianfeng; Zhao, Haijun; Wang, Jianjun; Xu, Shuai; Huang, Zong-hai

doi:10.1016/j.febslet.2015.05.056

Cited by 42 publications

(33 citation statements)

References 24 publications

(29 reference statements)

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“…The dysregulated expression of miRs has been found to correlate with NB growth, invasion and migration, thus reinforcing the importance of miR biology in NB-associated tumourigenesis (22,23). In the present study, t miR-451 was found to be downregulated in NB tissues, and tumours of patients with lower levels of miR-451 tended to be larger, poorly differentiated and associated with metastasis.…”

Section: A B C D Esupporting

confidence: 76%

MicroRNA-451 inhibits neuroblastoma proliferation, invasion and migration by targeting macrophage migration inhibitory factor

Zhou

Hao

2016

Molecular Medicine Reports

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Neuroblastoma (NB) is the most prevalent type of extracranial solid tumour in young children. To improve current understanding of the mechanisms, which modulate cancer cell proliferation, invasion and migration, investigations have focused on microRNAs (miRs), a class of small non‑coding RNAs, which post‑transcriptionally regulate gene expression during various crucial cell processes. The present study aimed to investigate the role of miR‑451 in NB. Human NB tissue and adjacent normal tissue were surgically removed, and the expression of miR‑451, and development and pathological characteristics of NB were investigated. The expression of miR‑451 was reduced in the NB tissue, compared with that in the adjacent tissue, and correlations between the reduction in miR‑451 and unfavourable variables included tumour size (P=0.0081), differentiation (P=0.0217), lymph node metastasis (P=0.0489), tumour‑node‑metastasis stage (0.0220) and distant metastases (P=0.0201). Transfection of the SK‑N‑SH and GI‑LA‑N NB cell lines with miR‑451 inhibited cell growth, invasion and migration. Furthermore, the present study demonstrated that macrophage migration inhibitory factor (MIF) was regulated directly by miR‑451 and was a critical mediator of the biological effects of miR‑451 in NB. The re‑expression of MIF markedly reversed the carcinogenic inhibitory property of miR‑451. These data provide a more detailed understanding of the essential role of miR‑451 in NB, which relies on regulation of the expression of MIF.

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Section: A B C D Esupporting

confidence: 76%

MicroRNA-451 inhibits neuroblastoma proliferation, invasion and migration by targeting macrophage migration inhibitory factor

Zhou

Hao

2016

Molecular Medicine Reports

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“…In gastric cancer, miR-362 expression was upregulated in tumor tissues and cell lines in comparision with that in normal gastric tissues and primary normal human gastric epithelial cells, respectively (32). These studies provided evidence to suggest that miR-362 was upregulated; however, in neuroblastoma, miR-362 expression level was reduced (33). In this study, we showed that miR-362 was significantly downregulated in cervical cancer.…”

Section: Discussionmentioning

confidence: 48%

“…Previous study also observed that miR-362 knockdown inhibited hepatocellular carcinoma cell proliferation, clonogenicity, migration and invasion in vitro as well as tumor growth and metastasis in vivo (31). Wu and his colleagues revealed that upregualtion of miR-362 repressed cell proliferation, migration and invasion of neuroblastoma in vitro, and decreased tumor growth of neuroblastoma in vivo (33). In gastric cancer, miR-362 knockdown inhibited cell proliferation, colony formation, and resistance to cisplatin-induced apoptosis (32).…”

Section: Discussionmentioning

confidence: 85%

MicroRNA-362 is downregulated in cervical cancer and inhibits cell proliferation, migration and invasion by directly targeting SIX1

Shi

Zhang

2016

Oncology Reports

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Cervical cancer is the second most common type of cancer in women accounting for 12% of all human cancers in the world. Mounting evidence demonstrates that microRNAs play important roles in the carcinogenesis and progression of cervical cancer. The aim of this study was to investigate the expression, roles and molecular mechanism of microRNA-362 (miR-362) in cervical cancer. According to the results, we found that expression level of miR-362 was significantly reduced in cervical cancer tissues and cell lines. Low miR-362 expression was correlated with FIGO stage, lymph node metastasis and vascular invasion in cervical cancer. Functional assays showed that restoration of miR-362 repressed cell proliferation, migration and invasion in cervical cancer. We also provided direct evidence that sineoculis homeobox homolog 1 (SIX1) was a direct target of miR-362 in cervical cancer, which was confirmed by bioinformatics analysis, luciferase reporter assay, qRT-PCR and western blot analysis. SIX1 was upregulated in cervical cancer and inversely correlated with miR‑362 expression in cervical cancer. In addition, SIX1 knockdown could simulate the roles of miR-362 overexpression on cell proliferation, migration and invasion of cervical cancer. Moreover, rescue experiments indicated that restoration of SIX1 was sufficient to abolish proliferation, migration and invasion induced by miR-362 overexpression in cervical cancer cells. The newly identified miR-362/SIX1 pathway provides insight into cervical cancer progression, and may represent a novel therapeutic target.

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“…miR-338-3p suppresses NB proliferation, invasion and migration through phosphatidylinositol-3,4,5-trisphosphate (PIP 3 )-dependent rac exchange factor 2 (6). miR-362-5p inhibits the proliferation and migration of NB cells by targeting phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2β (7). miR-145 regulates the gene expression of hypoxia-inducible factor 2α, thus inhibiting the growth, invasion, metastasis and angiogenesis of NB cells (8).…”

Section: Introductionmentioning

confidence: 99%

“…miR-145 regulates the gene expression of hypoxia-inducible factor 2α, thus inhibiting the growth, invasion, metastasis and angiogenesis of NB cells (8). Although multiple genetic and molecular lesions have been associated with NB tumorigenesis (6,7), the molecular mechanisms regulating NB remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Reduction of miR-21 induces SK-N-SH cell apoptosis and inhibits proliferation via PTEN/PDCD4

Wang

Yao

et al. 2017

Oncology Letters

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MicroRNA (miR/miRNA)-21 is a well-known oncogenic miRNA that is overexpressed in various types of tumors. The tumor-suppressor genes programmed cell death 4 (PDCD4) and phosphatase tensin homologue (PTEN), are targets of miR-21, and are underexpressed in several types of cancer. However, the expression of miR-21 and its target genes in neuroblastoma (NB) remains unclear. In the present study, a miR-21 inhibitor oligonucleotide was transfected into the SK-N-SH cell line, and the expression of miR-21, PTEN and PDCD4 was detected through quantitative polymerase chain reaction analysis. Western blotting was used to examine levels of PTEN, PDCD4 and caspase-3 proteins. The expression of PTEN and PDCD4 in the SK-N-SH cell line transfected with the miR-21 inhibitor was significantly increased compared with untransfected SK-N-SH and negative control-transfected cells. Cell proliferation was inhibited and the apoptotic ratio was significantly increased in miR-21 inhibitor-transfected cells compared with untransfected SK-N-SH and negative control-transfected cells. Western blot analysis revealed a significant increase in caspase-3 expression compared with untransfected SK-N-SH and negative control-transfected cells. The results of the present study indicate that miR-21 may serve an oncogenic role in the cellular processes underlying NB development and thus may be a novel therapeutic target for the treatment of patients with NB.

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miR‐362‐5p inhibits proliferation and migration of neuroblastoma cells by targeting phosphatidylinositol 3‐kinase‐C2β

Cited by 42 publications

References 24 publications

MicroRNA-451 inhibits neuroblastoma proliferation, invasion and migration by targeting macrophage migration inhibitory factor

MicroRNA-451 inhibits neuroblastoma proliferation, invasion and migration by targeting macrophage migration inhibitory factor

MicroRNA-362 is downregulated in cervical cancer and inhibits cell proliferation, migration and invasion by directly targeting SIX1

Reduction of miR-21 induces SK-N-SH cell apoptosis and inhibits proliferation via PTEN/PDCD4

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