Reduction of miR-21 induces SK-N-SH cell apoptosis and inhibits proliferation via PTEN/PDCD4

Wang, Zuopeng; Yao, Wei; Li, Kai; Zheng, Na; Cheng, Zheng; Zhao, Xiaolong; Zheng, Shan

doi:10.3892/ol.2017.6052

Cited by 20 publications

(11 citation statements)

References 30 publications

(35 reference statements)

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“…Usually one miRNA targets multiple genes. As PDCD4 has been frequently reported as a target of miR-21-5p, the role of miR-21-5p in PDCD4 expression in TSCC requires additional study (21). In the present study, the luciferase reporter assay revealed that PDCD4-UTR-pMIR (WT) was significantly increased by miR-21-5p inhibitors.…”

Section: Discussionmentioning

confidence: 99%

“…The PI3K/AKT pathway is closely associated with apoptosis. This pathway controls cell proliferation, growth, translation, migration and survival, and overactivation of this signaling pathway is associated with poor prognosis (21,22). Activated PI3K may activate downstream protein kinase AKT; Activated AKT is able to phosphorylate Bax, inactivate Bax and inhibit Bax and Bcl-2 to form dimers, thereby resulting in Bcl-2 dissociation and an anti-apoptotic effect (23,24).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

MicroRNA‑21‑5p targeting PDCD4 suppresses apoptosis via regulating the PI3K/AKT/FOXO1 signaling pathway in tongue squamous cell carcinoma

et al. 2019

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The aim of the present study was to analyze the role of microRNA (miRNA)-21-5p in tongue squamous cell carcinoma (TSCC), predict the target gene of miR-21-5p and provide novel strategies for gene therapy in TSCC treatment. The expression levels of miRNA-21-5p in TSCC tissues were analyzed using reverse transcription quantitative polymerase chain reaction, and the effects of miRNA-21-5p on cell proliferation, invasion and apoptosis and the expression levels of target protein PDCD4 in the Cal 27 and SCC9 cell lines were determined. PI3K/AKT/Forkhead Box O1 (FOXO1) pathway-associated protein expression levels were evaluated by western blot analysis. miRNA-21-5p was consistently upregulated in TSCC tissues compared with normal tissues. Inhibition of miR-21-5p inhibited cell proliferation and invasion, and promoted cell apoptosis. A luciferase reporter assay confirmed that PDCD4 was the target of miR-21-5p. Inhibition of miRNA21-5p suppressed the PI3K/Akt/FOXO1 signaling pathway. The results from the present study indicated that miR-21-5p-targeting PDCD4 suppresses apoptosis in human TSCC cell lines. This anti-apoptotic effect was achieved by regulating the PI3K/Akt/FOXO1 signaling pathway. These data represent the basis for a promising novel strategy for the treatment of TSCC.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

MicroRNA‑21‑5p targeting PDCD4 suppresses apoptosis via regulating the PI3K/AKT/FOXO1 signaling pathway in tongue squamous cell carcinoma

et al. 2019

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“…Eight of 10 upregulated circRNAs and two of five downregulated circRNAs were finally verified in 20 paired samples ( Figure 2 A and B ). Our previous study had shown that miR-21 plays a significant role in the regulation of proliferation and apoptosis in NB, 6 , 17 so we selected another seven circRNAs for analysis, whose predicted target miRNA was miR-21. Real-time qRT-PCR confirmed that three miR-21-related circRNAs, including circRNA-TBC1D4 (average FoldChange = 18.7; P = 0.0002), circRNA-NAALAD2 (average FoldChange = 5.6; P = 0.0032) and circRNA-TGFBR3 (average FoldChange = 23.3; P = 0.0079), were significantly downregulated in NB samples ( Figure 2C ).…”

Section: Resultsmentioning

confidence: 99%

“…Our previous study focused on the oncogenic gene miR-21 in NB and found that miR-21 may regulate the potential targets PTEN/PDCD4 to promote apoptosis. 6 , 17 In addition, miR-21 functions as a significant common carcinogen in a variety of cancers, such as gliomas, 23 colon cancer, 24 cutaneous malignant melanoma 25 and retinoblastoma. 26 Three miR-21-related circRNAs, circRNA-TBC1D4, circRNA-NAALAD2 and circRNA-TGFBR3, were confirmed to be overexpressed in adjacent adrenal tissue compared to tumor tissue and this was verified by qRT-PCR.…”

Section: Discussionmentioning

confidence: 99%

circRNA-TBC1D4, circRNA-NAALAD2 and circRNA-TGFBR3: Selected Key circRNAs in Neuroblastoma and Their Associations with Clinical Features

Lin¹,

Wang²,

Wang³

et al. 2021

CMAR

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Objective The roles of circRNAs in neuroblastoma (NB) are unclear. We used next-generation sequencing to detect the circRNA expression profiles in NB to identify the key circRNAs and analyzed the relationships between the circRNAs and clinical features. Methods Five paired neuroblastoma tumor and adjacent normal fetal adrenal medulla samples were collected for high-throughput RNA sequencing. Bioinformatics analysis was performed for functional annotation of the host genes of differentially expressed circRNAs. Validation of dysregulated circRNAs was performed by real-time quantitative reverse transcription polymerase chain reaction. The relationships between the key circRNAs and clinical features were analyzed. In addition, overexpression of key circRNAs in an NB cell line, as well as cell proliferation assays, colony formation assays and cell migration assays, was conducted to investigate the biological functions of key circRNAs. Results A total of 4704 differentially expressed circRNAs were found, including 2462 up-regulated and 2242 down-regulated circRNAs. According to our previous studies, the predicted target circRNAs of miR-21 involved in tumorigenic signaling pathways were selected, including circRNA-TBC1D4, circRNA-NAALAD2 and circRNA-TGFBR3. These circRNAs were associated with clinical features, and the circRNA expression was significantly lower (P < 0.05) in the NB tissues than in normal adrenal tissues. Overexpression of circRNA-TBC1D4 promotes NB cell migration, but not proliferation and colony-formation in vitro. Conclusion We suggest that circRNA-TBC1D4, circRNA-NAALAD2 and circRNA-TGFBR3 may be cancer suppressor genes, which act by sponging miR-21 in NB. Further investigations are needed to elucidate the underlying mechanism.

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“…Upregulation of miR-96 may suppress programmed cell death protein 4 (PDCD4) and subsequently decrease apoptosis. It is known that reduction of miR-21 promotes apoptosis by inhibiting the expression of phosphatase and tensin homolog (PTEN) and PDCD4 [ 38 ] . Down regulation of the pro-apoptotic factors PTEN and PDCD4 can increase expression of the cell survival factor Akt or protein kinase B, leading to a reduction in apoptosis in SCI.…”

Section: Potential Roles Of Mirnas In Scimentioning

confidence: 99%

Roles of miRNAs in spinal cord injury and potential therapeutic interventions

Almurshidi

Carver

Scott

et al. 2019

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Spinal cord injury (SCI) affects approximately 200,000 individuals per year worldwide. There are more than 27 million people worldwide living with long-term disability due to SCI. Historically, it was thought that the central nervous system (CNS) had little ability for regeneration; however, more recent studies have demonstrated potential for repair within the CNS. Because of this, there exists a renewed interest in the discovery of novel approaches to promote regeneration in the CNS including the spinal cord. It is important to know the roles of the microRNAs (miRNAs) in modulation of pathogenesis in SCI and the potentials of the miRNA-based clinical interventions for controlling post-injury symptoms and improving functional recovery. The miRNAs, which are non-coding RNAs with an average of 22 nucleotides in length, are post-transcriptional gene regulators that cause degradation of the target mRNAs and thus negatively control their translation. This review article focuses on current research related to miRNAs and their roles in modulating SCI symptoms, asserting that miRNAs contribute to critical post-SCI molecular processes including neuroplasticity, functional recovery, astrogliosis, neuropathic pain, inflammation, and apoptosis. In particular, miR-96 provides a promising therapeutic opportunity to improve the outcomes of clinical interventions, including the way SCI injuries are evaluated and treated.

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Reduction of miR-21 induces SK-N-SH cell apoptosis and inhibits proliferation via PTEN/PDCD4

Cited by 20 publications

References 30 publications

MicroRNA‑21‑5p targeting PDCD4 suppresses apoptosis via regulating the PI3K/AKT/FOXO1 signaling pathway in tongue squamous cell carcinoma

MicroRNA‑21‑5p targeting PDCD4 suppresses apoptosis via regulating the PI3K/AKT/FOXO1 signaling pathway in tongue squamous cell carcinoma

circRNA-TBC1D4, circRNA-NAALAD2 and circRNA-TGFBR3: Selected Key circRNAs in Neuroblastoma and Their Associations with Clinical Features

Roles of miRNAs in spinal cord injury and potential therapeutic interventions

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