MiR-3614-5p Is a Potential Novel Biomarker for Colorectal Cancer

Lin, Han; Sun, Yanjun; Lu, Cansheng; Ma, Chungeng; Shi, Jian; Sun, Dengqun

doi:10.3389/fgene.2021.666833

Cited by 12 publications

(10 citation statements)

References 39 publications

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“…Although no other studies have reported the role of miR-3614-3p in the pathology of BC, the -5p of this miR gene has been reported to be associated with colon cancer. From this reporting, miR-3614-5p was identified to suppress the proliferation of colon cancer cells through inhibition of the P53 pathway and p38MAPK pathway ( 10 ). However, other functional roles of miR-3614 in cancer development and pathology remain to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

miR-3614-3p suppresses cell aggressiveness of human breast cancer by targeting AKT3 and HDAC1 expression

Wang¹,

Jing²,

Li³

et al. 2022

Transl Cancer Res TCR

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Background MicroRNAs (miRNAs) regulate various pathophysiological functions and pathobiological progression in various cancers. Our recent study reported that miR-3614-3p significantly suppressed the proliferation of breast cancer (BC) cells by downregulating its host gene TRIM25 . However, other functional roles of miR-3614-3p migration and invasion in BC and its potential mechanisms are not clearly elucidated. Methods In this study, we investigated miR-3614-3p regulation of AKT3 and HDAC1 expression in BC. miR-3614-3p and AKT3/HDAC1 mRNA expression levels were determined using quantitative real-time PCR (qRT-PCR) in MCF-7 and MDA-MB-231 BC cells. The effects of miR-3614-3p on migration and invasion were measured using wound healing and transwell migration assays. In BC cells, miR-3614-3p levels were remarkably low, and AKT3 and HDAC1 mRNA and protein levels were high as assessed by qRT-PCR and western blot. Finally, we investigated the role of AKT3/HDAC1 using silent interfering RNA (siRNA) and confirmed the targeting of AKT3 and HDAC1 3' UTR through miR-3614-3p using a luciferase reporter assay. Results In the present study, we found that overexpression of miR-3614-3p markedly suppressed tumor cell invasion and migration independent of TRIM25 , whereas other target genes, AKT3 and HDAC1 , were involved. Moreover, we found that the resulting silencing of AKT3 and HDAC1 suppressed cell migration. Conclusions miR-3614-3p is an anti-oncogene that can suppress BC cells by targeting AKT3 and HDAC1 , revealing the potential role of miR-3614-3p in suppressing BC metastasis. Therefore, miR-3614 may act as a potential biomarker for BC prognosis.

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Section: Introductionmentioning

confidence: 99%

miR-3614-3p suppresses cell aggressiveness of human breast cancer by targeting AKT3 and HDAC1 expression

Wang¹,

Jing²,

Li³

et al. 2022

Transl Cancer Res TCR

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“…In the published data, miR‐3614‐5p is related to various types of cancer. For example, bioinformatics analysis was used to screen for new biomarkers for CRC, and miR‐3614‐5p was identified as a biomarker that predicts the survival and prognosis of patients with CRC 34 . miR‐3614‐5p is significantly downregulated in nonsmall cell lung cancer 35 .…”

Section: Discussionmentioning

confidence: 99%

“…For example, bioinformatics analysis was used to screen for new biomarkers for CRC, and miR-3614-5p was identified as a biomarker that predicts the survival and prognosis of patients with CRC. 34 miR-3614-5p is significantly downregulated in nonsmall cell lung cancer. 35 In hepatocellular carcinoma, miR-3614-5p inhibits malignant progression by regulating YY1.…”

Section: Rfc5 Knockdown Inhibits Crc Progression and May Affect The V...mentioning

confidence: 96%

RFC5, regulated by circ_0038985/miR‐3614‐5p, functions as an oncogene in the progression of colorectal cancer

Yao

Zhou

et al. 2023

Molecular Carcinogenesis

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Replication factor C 5 (RFC5) is involved in a variety of biological functions of cancer.However, the expression pattern of RFC5 and the underlying mechanisms in colorectal cancer (CRC) remain elusive. Here, we show that RFC5 is significantly upregulated in CRC tissues and cells. Patients with CRC and increased RFC5 levels have an unfavorable prognosis. RFC5 can promote the proliferation, migration, and invasion of CRC cells and inhibit the apoptosis of CRC cells. Additionally, upstream of RFC5, we constructed the competing endogenous RNA network and confirmed that RFC5 in this network was inhibited by miR-3614-5p by directly targeting its 3′-untranslated regions. We verified that circ_0038985, which is positively correlated with RFC5, directly targeted miR-3614-5p. Overexpression of circ_0038985 promoted CRC cell migration and invasion, and these effects were partially reversed by the reintroduction of miR-3614-5p. Moreover, we found that RFC5 may promote the vascular endothelial growth factor A (VEGFa)/vascular endothelial growth factor receptor 2 (VEGFR2)/extracellular signal-regulated protein kinase (ERK) pathway. The knockdown of RFC5 reduced CRC tumorigenesis in vivo.Collectively, these data demonstrate that the circ_0038985/miR-3614-5p/RFC5 axis plays a critical role in the progression of CRC, and RFC5 may promote CRC progression by affecting the VEGFa/VEGFR2/ERK pathway.

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“…7,8 In addition, an increasing number of studies have suggested the role of aberrant miRs as biomarkers in various cancers, including CC. [9][10][11][12] Notably, an earlier study on miRNA expression profiles in HPV infected CC patients has found that the expression levels of miR-3653 in HPV infected CC tissues were significantly higher than that in normal cervical tissues without HPV infection. 13 However, the relationship between aberrant expression of miR-3653 and HPV infection is unclear.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of miR-3653 is Associated with HPV Infection and Serves as a Biomarker in Patients with Cervical Cancer

Cui¹,

Zhang²,

Ruan³

et al. 2022

IJWH

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Background: Human papillomavirus (HPV) is a major cause of cervical cancer (CC) occurrence. This study aimed to explore whether abnormal microRNA (miR)-3653 is associated with HPV infection and to investigate the clinical value of miR-3653 in the diagnosis and prognosis of CC. Methods: Tumor tissues and adjacent non-cancerous tissues were collected from 136 patients with CC. Cervical tissues from 101 patients with uterine fibroids were collected as controls. The expression of miR-3653 was measured by quantitative real-time PCR. The ability of miR-3653 to discriminate between HPV positive (HPV+) and HPV negative (HPV-) CC patients, and to discriminate patients from controls was assessed by receiver operating characteristic analysis. Kaplan-Meier curves and Log rank tests were used to evaluate the relationship of miR-3653 with survival of CC patient. Whether miR-3653 could function as a prognostic indicator was evaluated by univariate and multivariate Cox analyses. Results: miR-3653, highly expressed in CC tissues, was associated with HPV infection, tumor diameter, International Federation of Gynecology and Obstetrics (FIGO) stage and lymph node metastasis in CC patients. Additionally, miR-3653 was increased in HPV+ controls, CC patients and CC cells. Moreover, miR-3653 could screen HPV+ controls, screen HPV+ patients and screen CC patients. Furthermore, miR-3653 was associated with the survival of CC patients (log-rank P < 0.001) and could serve as an independent prognostic indicator for CC patients. Conclusion: miR-3653, increased in CC, is related to HPV infection and may serve as a diagnostic and prognostic biomarker for CC patients.

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MiR-3614-5p Is a Potential Novel Biomarker for Colorectal Cancer

Cited by 12 publications

References 39 publications

miR-3614-3p suppresses cell aggressiveness of human breast cancer by targeting AKT3 and HDAC1 expression

miR-3614-3p suppresses cell aggressiveness of human breast cancer by targeting AKT3 and HDAC1 expression

RFC5, regulated by circ_0038985/miR‐3614‐5p, functions as an oncogene in the progression of colorectal cancer

Overexpression of miR-3653 is Associated with HPV Infection and Serves as a Biomarker in Patients with Cervical Cancer

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