RFC5, regulated by circ_0038985/miR‐3614‐5p, functions as an oncogene in the progression of colorectal cancer

Abstract

Replication factor C 5 (RFC5) is involved in a variety of biological functions of cancer.However, the expression pattern of RFC5 and the underlying mechanisms in colorectal cancer (CRC) remain elusive. Here, we show that RFC5 is significantly upregulated in CRC tissues and cells. Patients with CRC and increased RFC5 levels have an unfavorable prognosis. RFC5 can promote the proliferation, migration, and invasion of CRC cells and inhibit the apoptosis of CRC cells. Additionally, upstream of RFC5, we constructed the competing endogenous RNA network and confirmed that RFC5 in this network was inhibited by miR-3614-5p by directly targeting its 3′-untranslated regions. We verified that circ_0038985, which is positively correlated with RFC5, directly targeted miR-3614-5p. Overexpression of circ_0038985 promoted CRC cell migration and invasion, and these effects were partially reversed by the reintroduction of miR-3614-5p. Moreover, we found that RFC5 may promote the vascular endothelial growth factor A (VEGFa)/vascular endothelial growth factor receptor 2 (VEGFR2)/extracellular signal-regulated protein kinase (ERK) pathway. The knockdown of RFC5 reduced CRC tumorigenesis in vivo.Collectively, these data demonstrate that the circ_0038985/miR-3614-5p/RFC5 axis plays a critical role in the progression of CRC, and RFC5 may promote CRC progression by affecting the VEGFa/VEGFR2/ERK pathway.

Inhibition of Colorectal Cancer Cell Proliferation using Multi-Glycoside of Tripterygium wilfordii

“…With the advancement of medical technology, molecular targeted therapy has become the focus of research, but the morbidity and mortality of CRC are still increasing annually [13] . Convincing evidence shows that the active ingredients extracted 3614-5p might suppress the growth and metastasis of multiple tumors, containing CRC cells [10,11] . Herein, our study's first step documented that PRR34-AS1 expression was significantly reduced in GTW-triggered CRC cells, and miR-3614-5p content was increased.…”

“…Here, based on StarBase analysis, there are some binding sites between PRR34-AS1 and microRNA (miR)-3614-5p. Beyond that, miR-3614-5p has been confirmed as an underlying novel biomarker for CRC and participates in the modulation of cell migration [10,11] . Therefore, the purpose of this study is to validate the role of GTW block CRC progression via the PRR34-AS1/miR-3614-5p axis.…”

RFC2 promotes aerobic glycolysis and progression of colorectal cancer

“…RFC4 is identified as a radioresistance factor that promotes NHEJ-mediated DNA repair in CRC cells [ 16 ]. circ_0038985/miR-3614-5p RFC5 promotes the progression of CRC [ 17 ]. RFC2 promotes diffuse lower-grade gliomas progression and correlates with the immune infiltration of lower-grade gliomas [ 18 ].…”