miR-335 suppresses migration and invasion by targeting ROCK1 in osteosarcoma cells

Wang, Yong; Zhao, Wei; Fu, Qin

doi:10.1007/s11010-013-1786-4

Cited by 89 publications

(89 citation statements)

References 27 publications

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“…Based on bioinformatical predication, a luciferase reporter assay was performed and PAX6 was identified as a novel target of miR-335, which has been demonstrated to be implicated in various types of cancer (14)(15)(16)(17)(18)(19)(20)(21)(22). However, the detailed role of miR-335 in the development and progression of various types of cancer remains controversial.…”

Section: Discussionmentioning

confidence: 99%

“…However, the detailed role of miR-335 in the development and progression of various types of cancer remains controversial. For instance, it has been suggested that miR-335 may function as a tumor suppressor in osteosarcoma (14), however, it may act as an oncogene in meningiomas (21). These findings suggest that the exact regulatory function of miR-335 in tumorigenesis is tissue specific, since different microenvironments in various types of cancer may have different effects on the expression and activity of miR-335.…”

Section: Discussionmentioning

confidence: 99%

“…miR-335 has been demonstrated to be implicated in multiple types of malignant tumor, including small cell lung cancer, osteosarcoma, ovarian cancer, prostate cancer, hepatocellular carcinoma, meningioma and gastric cancer (14)(15)(16)(17)(18)(19)(20)(21)(22). Recently, Jiang et al have suggested that miR-335 upregulation is associated with advanced tumor progression in glioma and is an independent marker for the predication of the clinical outcome of patients with glioma (23).…”

Section: Introductionmentioning

confidence: 99%

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PAX6, a novel target of miR-335, inhibits cell proliferation and invasion in glioma cells

Cheng

Cao²,

Chen

et al. 2014

Molecular Medicine Reports

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Abstract. Paired box 6 (PAX6), a highly conserved transcription factor, is important in glioma. However, the molecular mechanisms involved remain unclear. The present study demonstrated that the expression of PAX6 was significantly reduced with the malignancy of glioma and also identified PAX6 as a novel target of microRNA (miR)-335, which was significantly upregulated in glioma. The inhibition of miR-335 increased the protein expression of PAX6, whereas the upregulation of miR-335 suppressed its expression in human glioma U251 and U87 cells. Furthermore, upregulation of miR-335 promoted U251 cell proliferation, colony formation and invasion, which was reversed by the overexpression of PAX6. Furthermore, the present study demonstrated that the effect of miR-335 on U251 cell invasion was via the modulation of matrix metalloproteinase (MMP)-2 and MMP-9 expression by targeting PAX6. In conclusion, the present study demonstrated that PAX6, as a novel target of miR-335, has an anti-oncogenic function in glioma, and thus PAX6 may serve as a therapeutic target for glioma.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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PAX6, a novel target of miR-335, inhibits cell proliferation and invasion in glioma cells

Cheng

Cao²,

Chen

et al. 2014

Molecular Medicine Reports

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“…Furthermore, increased levels of miR-128 are negatively correlated with PTEN levels, and increased proliferation of MG63 and U2OS OS cells (28). In contrast to these oncogene-like miRNA functions, miR-335 and miR-340 function as tumor suppressors by targeting the Rho-associated, coiled-coil containing protein kinase 1 gene and, thereby, inhibit OS cell migration and invasion (29,30). miR-126 inhibits the proliferation of OS cells by targeting sirt1, a histone deacetylase (31).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA screening identifies circulating microRNAs as potential biomarkers for osteosarcoma

Li¹,

Zhang²,

Liu³

et al. 2015

Oncology Letters

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Abstract. MicroRNAs (miRNAs) are a family of small non-protein coding RNAs, which regulate the expression of a wide variety of genes at the post-transcriptional level to control numerous biological and pathological processes. Various circulating miRNAs have been identified as potential diagnostic and prognostic biomarkers in multiple types of cancer and disease. The aim of the present study was to identify potential miRNA biomarkers for the early diagnosis and relapse prediction of osteosarcoma (OS). miRNA profiling was performed on serum from patients with osteosarcoma and healthy controls. All putative miRNAs were verified by reverse transcription-quantitative polymerase chain reaction analysis of 20 pre-therapeutic OS patients and 20 healthy individuals. The expression of miR-106a-5p, miR16-5p, miR-20a-5p, miR-425-5p, miR451a, miR-25-3p and miR139-5p was demonstrated to be downregulated in the serum of OS patients when compared with that of the healthy controls. Receiver-operating characteristic curve analyses indicated that these 7 miRNAs may be used as diagnostic biomarkers with the ability to discriminate between the healthy cohort and patients with OS. These results provide novel insights into the use of miRNAs in early blood screening for OS.

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“…In addition, recent studies have employed qRT-PCR for osteosarcoma research. For instance, down-regulation of miR-335 is significantly associated with lymph node metastasis, indicating that this miRNA inhibits cell migratory and invasive properties [79] .…”

Section: Mirna Analysismentioning

confidence: 99%

The advancements of genomics and data integration in sarcoma research

et al. 2017

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Abstract:In the age of big data, genomics and clinical research have reached a crossroads. A wealth of data is being generated, but it is becoming increasingly complicated to analyze these data to extract meaningful results. The ability to understand biological systems holistically has unprecedented potential to transform how cancers are treated. Recent major advances leading biomedical research towards "systems medicine" have been fueled by high-throughput platforms, such as microarrays and next-generation sequencing, which can capture vast amounts of data in different genomic spaces. Unfortunately, because of high dimensionality and complex relationships among these data, inferring comprehensive and useful biological models has proven computationally and statistically challenging. However, novel bioinformatic methods for data integration of cancer genomic datasets have been developed. In this review, we will describe the applications of various genomic approaches in sarcoma research and introduce bioinformatic methods for data integration. With the continuing evolution of technological and bioinformatic methodologies, the application of big data within clinics and hospitals will ultimately result in significant improvements on how cancers are detected and treated.Keywords: omics; genomics; sarcomas; sequencing; microarray; data integration; bioinformatics; big data

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miR-335 suppresses migration and invasion by targeting ROCK1 in osteosarcoma cells

Cited by 89 publications

References 27 publications

PAX6, a novel target of miR-335, inhibits cell proliferation and invasion in glioma cells

PAX6, a novel target of miR-335, inhibits cell proliferation and invasion in glioma cells

MicroRNA screening identifies circulating microRNAs as potential biomarkers for osteosarcoma

The advancements of genomics and data integration in sarcoma research

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