MicroRNA screening identifies circulating microRNAs as potential biomarkers for osteosarcoma

Li, Hui; Zhang, Kun; Liu, Li‑Hong; Ouyang, Yurong; Guo, Haipeng; Zhang, Hanchong; Bu, Jie; Xiao, Tao

doi:10.3892/ol.2015.3378

Cited by 38 publications

(28 citation statements)

References 62 publications

(58 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…11 MiRNAs display several properties that make them potentially valuable biomarkers including stability in body fluids, resistance to endogenous RNase activity, prolonged life at room temperature, and resistance to multiple freeze-thaw cycles. 12 A growing body of evidence shows a different pattern of expression of circulating miRNAs between cancer patients and Identification of plasma microRNAs as new potential biomarkers with high diagnostic power in human cutaneous melanoma healthy donors 11,[13][14][15][16][17][18][19] ; however, only a limited number of studies have been focused on melanoma.…”

Section: Introductionmentioning

confidence: 99%

Identification of plasma microRNAs as new potential biomarkers with high diagnostic power in human cutaneous melanoma

et al. 2017

View full text Add to dashboard Cite

Melanoma is a devastating disease with few therapeutic options in the advanced stage and with the urgent need of reliable biomarkers for early detection. In this context, circulating microRNAs are raising great interest as diagnostic biomarkers. We analyzed the expression profiles of 21 selected microRNAs in plasma samples from melanoma patients and healthy donors to identify potential diagnostic biomarkers. Data analysis was performed using global mean normalization and NormFinder algorithm. Linear regression followed by receiver operating characteristic analyses was carried out to evaluate whether selected plasma miRNAs were able to discriminate between cases and controls. We found five microRNAs that were differently expressed among cases and controls after Bonferroni correction for multiple testing. Specifically, miR-15b-5p, miR-149-3p, and miR-150-5p were up-regulated in plasma of melanoma patients compared with healthy controls, while miR-193a-3p and miR-524-5p were down-regulated. Receiver operating characteristic analyses of these selected microRNAs provided area under the receiver operating characteristic curve values ranging from 0.80 to 0.95. Diagnostic value of microRNAs is improved when considering the combination of miR-149-3p, miR-150-5p, and miR-193a-3p. The triple classifier had a high capacity to discriminate between melanoma patients and healthy controls, making it suitable to be used in early melanoma diagnosis.

show abstract

Section: Introductionmentioning

confidence: 99%

Identification of plasma microRNAs as new potential biomarkers with high diagnostic power in human cutaneous melanoma

et al. 2017

View full text Add to dashboard Cite

show abstract

“…miR‐20a‐5p, the strongest miRNA associated with hand grip strength in our study, is a member of the miR‐17 family known to have many biologic functions including a role in hepatic insulin resistance (Fang et al ., ). Its function in muscle is not well studied nor completely understood; however, recent work suggests a potential role as a biomarker for osteosarcoma (Li et al ., ). Several miRNAs among the top 15 hand grip strength‐associated miRNAs have links to skeletal muscle.…”

Section: Discussionmentioning

confidence: 97%

Cross‐sectional relations of whole‐blood miRNA expression levels and hand grip strength in a community sample

et al. 2017

View full text Add to dashboard Cite

SummaryMicroRNAs (miRNAs) regulate gene expression with emerging data suggesting miRNAs play a role in skeletal muscle biology. We sought to examine the association of miRNAs with grip strength in a community‐based sample. Framingham Heart Study Offspring and Generation 3 participants (n = 5668 54% women, mean age 55 years, range 24, 90 years) underwent grip strength measurement and miRNA profiling using whole blood from fasting morning samples. Linear mixed‐effects regression modeling of grip strength (kg) versus continuous miRNA ‘Cq’ values and versus binary miRNA expression was performed. We conducted an integrative miRNA–mRNA coexpression analysis and examined the enrichment of biologic pathways for the top miRNAs associated with grip strength. Grip strength was lower in women than in men and declined with age with a mean 44.7 (10.0) kg in men and 26.5 (6.3) kg in women. Among 299 miRNAs interrogated for association with grip strength, 93 (31%) had FDR q value < 0.05, 54 (18%) had an FDR q value < 0.01, and 15 (5%) had FDR q value < 0.001. For almost all miRNA–grip strength associations, increasing miRNA concentration is associated with increasing grip strength. miR‐20a‐5p (FDR q 1.8 × 10−6) had the most significant association and several among the top 15 miRNAs had links to skeletal muscle including miR‐126‐3p, miR‐30a‐5p, and miR‐30d‐5p. The top associated biologic pathways included metabolism, chemokine signaling, and ubiquitin‐mediated proteolysis. Our comprehensive assessment in a community‐based sample of miRNAs in blood associated with grip strength provides a framework to further our understanding of the biology of muscle strength.

show abstract

“…OS is the most common primary malignant bone tumor. Studies suggest that this tumor is associated with increased levels of circulating miRNAs (including 14q32 miRNAs, miR-95-3p, miR-300, miR-101, miR-542-3p, miR-196a, miR-196b, and miR-491) and with decreased levels of miR-17, miR-497, miR-106a-5p, miR-16-5p, miR-20a-5p, miR-425-5p, miR-451a, miR-25-3p, and miR-139-5p [26][27][28][29][30][31]47,51,52]. Of these, serum miR-199a-5p concentrations are significantly higher in OS patients than in controls, and are significantly lower in post-operative samples than in preoperative samples [53].…”

Section: Discussionmentioning

confidence: 99%

Signatures of circulating microRNA in four sarcoma subtypes

Koseła-Paterczyk¹,

Paziewska²,

Kulecka³

et al. 2020

J. Cancer

View full text Add to dashboard Cite

Background: Sarcomas are rare malignant tumors of mesenchymal origin. The discovery of circulating biomarkers with high diagnostic value could supplement diagnosis of this heterogenous group of tumors. The aim of this study was to identify the profiles of circulating miRNA (c-miRNAs) in four groups of common bone and soft tissue sarcomas. Methods: At the time of diagnosis, blood samples were collected from 86 patients: 36 with locally advanced/unresectable/metastatic gastrointestinal stromal tumor (GIST) who received first-line treatment with imatinib; 16 with locally advanced osteosarcoma (OS); 26 with locally advanced synovial sarcoma (SS); and eight with locally advanced Ewing sarcoma (ES). In addition, samples were collected from 30 healthy controls. C-miRNAs were isolated using a miRCURY RNA Isolation Kit, followed by preparation of cDNA libraries and sequencing on the Ion Proton platform. Results: Pair-wise comparisons identified 156 unique c-miRNAs (adjusted P-value < 0.05) showing significant dysregulation between controls and patients; of these, 24, 36, 42, and 99 differentiated controls from pretherapeutic OS, SS, ES, and GIST, respectively. Ten c-miRNAs were commonly altered in at least three sarcoma types. Receiver operating characteristic curves and area under the curve (ROC-AUC) analyses revealed that a four-miRNA diagnostic classifier was able to differentiate controls from ES, GIST, OS, and SS, with AUC-ROC values of 1, 0.97, 0.95, and 0.94, respectively. Conclusions: Aberrant miRNA expression signatures were identified in serum from patients with four different sarcoma subtypes. Differences in miRNA expression profiles between sarcoma patients and healthy volunteers suggest that miRNAs may play a role in sarcoma development.

show abstract

MicroRNA screening identifies circulating microRNAs as potential biomarkers for osteosarcoma

Cited by 38 publications

References 62 publications

Identification of plasma microRNAs as new potential biomarkers with high diagnostic power in human cutaneous melanoma

Identification of plasma microRNAs as new potential biomarkers with high diagnostic power in human cutaneous melanoma

Cross‐sectional relations of whole‐blood miRNA expression levels and hand grip strength in a community sample

Signatures of circulating microRNA in four sarcoma subtypes

Contact Info

Product

Resources

About