miR‑335‑5P contributes to human osteoarthritis by targeting HBP1

Abstract

MicroRNA (miR)-335-5P has the ability to regulate chondrogenic differentiation and promote chondrogenesis in mouse mesenchymal stem cells. It is also abnormally elevated in human osteoarthritic chondrocytes. However, the biological function of miR-335-5P in osteoarthritis (OA) is not well understood. The present study investigated the mechanism of miR-335-5P in the pathogenesis of OA. To investigate the effect of miR-335-5P on the pathogenesis of OA in vitro, a miR-335-5P mimic and inhibitor were transfected into chondrocytes. Cell Counting kit-8 assay and flow cytometry were used to observe the effects of miR-335-5P on chondrocyte apoptosis and the expression of cartilage-specific genes, such as aggrecan, collagen II, matrix metalloproteinase 13 and collagen X, were detected by reverse transcription-quantitative PCR and western blot analysis. Moreover, the current study assessed whether HMG-box transcription factor 1 (HBP1) is a novel target of miR-335-5P with dual luciferase reporter assays. Finally, a rescue experiment was used to prove the regulation between miR-335-5P and HBP1. The results revealed that HBP1 was a novel target of miR-335-5P, and that miR-335-5P mediated the apoptosis of chondrocytes and changes in cartilage-specific genes via targeting HBP1. Overall, the present study revealed that miR-335-5P mediated the development of OA by targeting the HBP1 gene and promoting chondrocyte apoptosis. These data suggested that miR-335-5P may be used to develop novel early-stage diagnostic and therapeutic strategies for OA.

LncRNA INPP5F ameliorates stress‐induced hypertension via the miR‐335/Cttn axis in rostral ventrolateral medulla

“…MiR‐335, which was remarkably downregulated in breast cancer, suppressed tumor growth by targeting Sdc1 60 . Moreover, it was also implicated in many other grave illnesses, such as type 2 diabetes, osteoarthritis, and polycystic ovary syndrome 61–63 . The authors' previous study proved that miR‐335 could serve as an apoptosis enhancer by inhibiting Sphk1 to increase the sympathetic vasoconstriction involved in SIH 25 .…”

Elevated miR-145-5p is Associated with Chronic Obstructive Pulmonary Disease (COPD) Associated Skeletal Muscle Dysfunction and Triggers Apoptotic Cell Death in C2C12 Myotubes

“…Dysregulation of miR-145-5p has been reported to be involved in the development of many diseases by regulating cell viability and apoptosis. [12-15, 17, 21, 29] , and it was previously reported that miR-145-5p promoted cell apoptosis in myocardial infarction [20] , osteoarthritis [30] and periodontal disease [31] , moreover, miR-145-5p inhibited cell viability and metastasis in LPS-treated HUVECs [32] , and it also repressed the proliferation and induced apoptosis in gastric cancer [33] , diabetes [34] and ovarian cancer [35] . However, the expression and function of miR-145-5p in COPD-associated skeletal muscle atrophy have not been reported.…”

Immune Response Serves as a Bridge between Abnormal Lipid and Bone Metabolism: A Differential Expression Genes Profile Analysis Based on Clinical Data-mining

“…For example, the hsa-miR-335-5p was signi cantly down-regulated under osteoinductive conditions in bone marrow-derived mesenchymal stem cells (MSCs) (Tornero-Esteban et al, 2015). Moreover, hsa-mir-335-5p association with multiple in ammation-related diseases, including OA (Lu et al, 2021), non-alcoholic fatty liver disease, etc, was described (Fan et al, 2021). Also, mir-335-5p and hsa-mir-26b-5p were found to in uence the differentiation of human MSCs (Jiang et al, 2022).…”