LncRNA INPP5F ameliorates stress‐induced hypertension via the miR‐335/Cttn axis in rostral ventrolateral medulla

Abstract

Hypertension is a condition wherein the blood vessel has persistently elevated pressure (defined as blood pressure ≥ 140/90 mmHg). It greatly increases the risk of many other disorders, such as stroke and heart failure. 1,2 The worldwide hypertensive population continues to soar, and more than 1.56 billion adults are expected to have hypertension in 2025. 3 Several factors, such as unhealthy diet, have been linked to hypertension risk. 4 Studies have suggested that chronic exposure to stressors, such as job strain, could

IFN-γ deficiency in the rostral ventrolateral medulla contributes to stress-induced hypertension by impairing microglial synaptic engulfment

“…The pathogenesis of SIH is closely related to RVLM neuronal hyperexcitation [9,10,29,30]. Our study confirmed that IFN-γ/CCL2 deficiency reduced microglial synaptic engulfment, resulting in increased synaptic density and enhanced neuronal excitability in the RVLM, which is involved in the pathogenesis of SIH.…”

“…The pathogenesis of SIH is closely related to RVLM neuronal hyperexcitation [9,10,29,30]. Our study confirmed that IFNg/CCL2 deficiency reduced microglial synaptic engulfment, FIGURE 2 Chronic stress induced an increase in the blood pressure, heart rate, renal sympathetic nerve activity, and neuronal activity of rats.…”

CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla

“…Following the standard rat atlas, the RVLM tissues were extracted by punching coronal sections [ 23 ]. cDNA libraries were generated as previously described [ 24 ]. In brief, TRIzol reagent (Invitrogen, USA) was utilized for the extraction of total RNA from RVLM tissues following the manufacturer's instructions.…”

“…The method of intra-RVLM microinjection has been described in the authors' previous studies [ 22 , 24 ]. In brief, the rats were anaesthetized with isoflurane as described above and placed in prone position, and their heads were fixed in a stereotaxic apparatus (RWD Life Science, China).…”

“…In accordance with the rat brain atlas [ 23 ], they were microinjected into the bilateral RVLM (located 3.7–4.0 mm caudal to lambdoid suture, 2 mm lateral to the midline, and 8.0 mm ventral to the surface of the dura) of rats at 0.1 μL/side by a glass micropipette. The diagram used to demonstrate the process of confirming the correct positioning of RVLM was drawn and described in the authors’ previous study [ 24 ]. The surgical incision was sutured after microinjection.…”