miR-330-5p Targets Tyrosinase and Induces Depigmentation

Rambow, Florian; Bechadergue, Audrey; Saintigny, Gaëlle; Morizot, Frédérique; Mahé, Christian; Larue, Lionel

doi:10.1038/jid.2014.231

Cited by 21 publications

(17 citation statements)

References 15 publications

(15 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because our previously miRNAs array also identified significant differential expression of miR-330-5p in the skin of different coat colors of animals [18,19]. Other report indicated that keratinocytes produce miR-330-5p [27] and miR-330-5p downregulated the expression of TYR that plays a key role in pigmentation [28,29]. Whether and how miR-330-5p regulates TYR in melanocytes to regulate pigmentation is not clear.…”

Section: Introductionmentioning

confidence: 94%

“…It was reported that MITF regulates gene expression [34]. To evaluate whether TYR [28] is the target gene of miR-330-5p, we measured the expression of TYR, TYRP1, TYRP2 and MITF in melanocytes treated with different exosomes. We found that the expression levels of TYR, TYRP1, TYRP2 and MITF proteins and genes were downregulated in the exosomes overexpressed with the miR-330-5p group compared with the control (Fig.…”

Section: Exosomes Carry Mir-330-5p That Regulate Melanocyte Pigmentationmentioning

confidence: 99%

See 1 more Smart Citation

Exosomal miRNA derived from keratinocytes regulates pigmentation in melanocytes

Liu

Gao

et al. 2019

Journal of Dermatological Science

View full text Add to dashboard Cite

Background: Pigmentation is controlled by complex mechanisms. Evidence suggests that miRNAs can regulate pigmentation. However, the mechanism has not been fully elucidated. Objective In this study, we revealed a novel mechanism that regulates pigmentation involving exosomes, miRNAs and the crosstalk between keratinocytes and melanocytes. Methods: The expression and localization of exosome specific marker TSG101 in keratinocytes and melanocytes; Changes of melanin content in melanocytes after co-culture of exosome and melanocytes; Expression changes of target gene TYR and its related genes and inhibitory effect of miR-330-5p on pigmentation were studied by using various molecular biological techniques. Results: In this experiment, we used miR-330-5p in keratinocytes to verify the effect of keratinocyte derived exosome on melanocyte pigmentation. First, we found that keratinocytes secrete exosomes carrying miR-330-5p; moreover, greater miR-330-5p expression was found in exosomes derived from keratinocytes that overexpressed miR-330-5p. Second, we found that exosomes derived from keratinocytes with overexpression of miR-330-5p caused a significant increase in miR-330-5p in melanocytes. Finally, exosomes derived from keratinocytes that overexpressed miR-330-5p induced a significant decrease in the production of melanin and expression of TYR in melanocytes. Meanwhile, we overexpressed miR-330-5p in melanocytes, which also proved the inhibitory effect of miR-330-5p on pigmentation. Conclusion: These findings suggest that keratinocytes crosstalk with melanocytes in the epidermal melanin unit via exosomal miRNAs. These studies reveal an important role of exosomes in melanocyte pigmentation, which opens a new pathway of melanogenesis.

show abstract

Section: Introductionmentioning

confidence: 94%

Section: Exosomes Carry Mir-330-5p That Regulate Melanocyte Pigmentationmentioning

confidence: 99%

Exosomal miRNA derived from keratinocytes regulates pigmentation in melanocytes

Liu

Gao

et al. 2019

Journal of Dermatological Science

View full text Add to dashboard Cite

show abstract

“…MiRNAs can bind with partial complementarity to sequences in the 3' untranslated region (3'-UTR) of target mRNAs for the regulation of gene expression. A growing number of events have shown that miRNAs are crucial for skin homeostatic maintenance (Table 1) [37][38][39][40][41][42][43][44][45][46][47][48][49][50][51] .…”

Section: Mirna In Vitiligomentioning

confidence: 99%

“…MiRNAs have been implicated in targeting TYR or MITF, which are potentially involved in the pigmentation process, to regulate their expression levels. For example, miR-434-5p and miR-330-5p are upstream regulators of TYR expression, suggesting that these miRNAs could be used as potential therapeutic targets for pigmentation 40,41,53 .…”

Section: Mirnas Associated With Melanocyte Proliferation Differentiamentioning

confidence: 99%

The Role of MicroRNAs in Vitiligo: Regulators and Therapeutic Targets

2020

Ann Dermatol

View full text Add to dashboard Cite

Vitiligo is an acquired skin disorder clinically characterized by the progressive appearance of white maculae due to a loss of functioning epidermal melanocytes. Studies have shown that microRNAs (miRNAs) modulate cellular differentiation, proliferation and apoptosis, including immune cell and melanocyte development and functions. The role of miRNAs in the pathogenesis of several immune-related diseases has been explored. Novel approaches to target miRNAs have recently emerged allowing modulation of miRNAs levels in diverse pathological processes, thus making them promising targets for molecular-based diagnostics and therapy. Here, we report the present status of research on miRNAs expression and functional alterations in vitiligo, in order to more fully understand the role of these molecules in vitiligo pathology.

show abstract

“…Another miRNA that could potentially be applied to treating aberrant skin pigmentation is miR-211, which was shown to target transforming growth factor (TGF)-β receptor in an MITF-dependent fashion [ 64 ]. Others include miR-25, miR-125b, miR-137, and miR-182, all of which target MITF; miR-330-5p, which targets TYR; and miR-145, which is a key regulator of many genes in the pigmentation process, such as TYR, TRP1, and MITF [ 65 , 66 , 68 – 71 ]. In cell culture, it was shown that miR-155 contributed to the pathogenesis of vitiligo by downregulating multiple genes associated with melanogenesis, including suppressor of cytokine signaling 1 (SOCS1) and TRP1, and that increased levels of miR-155 contributed to development of the skin disease [ 72 ].…”

Section: Introductionmentioning

confidence: 99%

Advances in the Application and Impact of MicroRNAs as Therapies for Skin Disease

Lawrence¹,

Ceccoli²

2017

BioDrugs

View full text Add to dashboard Cite

The advent of RNA interference (RNAi) technology has profoundly impacted molecular biology research and medicine but has also advanced the field of skin care. Both effector molecules of RNAi, short-interfering RNA molecules and microRNAs (miRNAs), have been explored for their relative impact and utility for treating a variety of skin conditions. These post-transcriptional RNA regulatory molecules down-modulate protein expression through targeting of the 3′ untranslated regions of messenger RNAs, leading to their degradation or repression through sequestration. As researchers hunt for genetic linkages to skin diseases, miRNA regulators have emerged as key players in the biology of keratinocytes, fibroblasts, melanocytes, and other cells of the skin. Herein, we attempt to coalesce the current efforts to combat various skin disorders and diseases through the development of miRNA-based technologies.

show abstract

miR-330-5p Targets Tyrosinase and Induces Depigmentation

Cited by 21 publications

References 15 publications

Exosomal miRNA derived from keratinocytes regulates pigmentation in melanocytes

Exosomal miRNA derived from keratinocytes regulates pigmentation in melanocytes

The Role of MicroRNAs in Vitiligo: Regulators and Therapeutic Targets

Advances in the Application and Impact of MicroRNAs as Therapies for Skin Disease

Contact Info

Product

Resources

About