MiR‐329 suppresses osteosarcoma development by downregulating Rab10

Jiang, Weihang; Liu, Jin; Xu, Tianyang; Xiao, Yu

doi:10.1002/1873-3468.12337

Cited by 46 publications

(63 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subsequently, we found that Lentivirus-mediated RAB10 knockdown markedly inhibited HCC cell proliferation in vitro , which was consistent with other study in osteosarcoma [27]. The total cell number and cell growth rate in shRAB10 lentivirus infected group were significantly decreased or slowed down compared with those in the NC lentivirus infected group.…”

Section: Discussionsupporting

confidence: 90%

RAB10 overexpression promotes tumor growth and indicates poor prognosis of hepatocellular carcinoma

Wang

Jia

et al. 2017

Oncotarget

View full text Add to dashboard Cite

Hepatocellular carcinoma (HCC), one of the most common and lethal cancers worldwide, has a high recurrence rate with current treatment modalities. Identifying biomarkers for early diagnosis and discovering new sufficient molecular targets for the development of targeted therapies are urgently needed. RAB10, a member of the RAS family, has been shown to be highly expressed in HCC. However, the function of RAB10 in HCC is less studied. Here we report that RAB10 acts as an oncogene in HCC. The shRNA-mediated knockdown of RAB10 significantly reduced the proliferation of HCC cells and colony formation, induced cell cycle arrest at G0/G1 phase and increased apoptosis in vitro. In addition, RAB10 knockdown suppressed HCC growth in nude mice. Moreover, RAB10 silencing decreased the phosphorylation of InsR, Met/HGFR, Ron/MST1R, Ret, c-Kit/SCFR, EphA3, EphB4, Tyro3/Dtk, Axl, Tie2/TEK, VEGFR2/KDR, Akt/PKB/Rac, S6 Ribosomal Protein and c-Abl, while the phosphorylation of HSP27, p38 MAPK, Chk2 and TAK1 increased significantly. These results suggest that RAB10 regulates cell survival and proliferation through multiple oncogenic, cell stress and apoptosis pathways. More importantly, high RAB10 expression levels in HCC cells correlated with a poor prognosis in HCC patients. Therefore, our findings revealed an oncogenic role for RAB10 in the pathogenesis of HCC and that RAB10 is a potential molecular target or a biomarker for HCC.

show abstract

Section: Discussionsupporting

confidence: 90%

RAB10 overexpression promotes tumor growth and indicates poor prognosis of hepatocellular carcinoma

Wang

Jia

et al. 2017

Oncotarget

View full text Add to dashboard Cite

show abstract

“…Resumption expression of miR-329 decreased tumor cell invasion capacity (32). Besides, miR-329 was lowly expressed and involved in occurrence and development of gastric cancer (33), pancreatic cancer (34), non-small cell lung cancer (35) and osteosacroma (36). These findings suggested that miR-329 may be exploited as a potent therapeutic methods for the treatments of specific cancers.…”

Section: Discussionmentioning

confidence: 95%

MicroRNA-329 serves a tumor suppressive role in colorectal cancer by directly targeting transforming growth factor beta-1

Li¹,

Huang²,

Wen³

et al. 2017

Molecular Medicine Reports

View full text Add to dashboard Cite

Colorectal cancer (CRC) is the third most common type of diagnosed cancer and the fourth leading cause of cancer‑associated mortalities worldwide. Increasing studies have demonstrated that the deregulation of microRNAs (miRNAs or miRs) is associated with the occurrence and development of multiple types of human cancer, including CRC. miR‑329 has been identified to be downregulated in various types of cancer; however, its expression pattern, functions and mechanisms in CRC remain unclear. The present study demonstrated that miR‑329 was lowly expressed in CRC tissue samples and cell lines. Low expression of miR‑329 was correlated with tumor‑node‑metastasis stage and lymph node metastasis in patients with CRC. In vitro experiments revealed that resumption expression of miR‑329 suppressed cell proliferation and invasion in CRC. Furthermore, the results of the present study indicated that miR‑329 targets transforming growth factor‑β1 (TGF‑β1) directly in vitro. TGF‑β1 was demonstrated to be upregulated in CRC tissue samples and inversely correlated with miR‑329 expression. Upregulation of TGF‑β1 was able to partially counteract the antitumor roles of miR‑329 on CRC cell proliferation and invasion. The results of the current study revealed that miR‑329 suppresses CRC cell proliferation and invasion through targeting TGF‑β1, thus suggesting that targeting miR‑329/TGF‑β1 may provide a novel effective therapeutic approach for the treatment of patients with CRC.

show abstract

“…The first eight of the nineteen targets, PIAS3, p27, RAB10, DBF4, DUSP14, RBPMS, PDPN and CLTC, were considered to be closely associated with cancer progression after a literature review. Among them, RAB10, a member of the RAS superfamily, might function as an oncogenic small GTPase in OS26. Kunita A, et al .…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-199a-5p promotes tumour growth by dual-targeting PIAS3 and p27 in human osteosarcoma

Wang

Guo

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Osteosarcoma (OS) is the most common primary bone malignancy and remains a leading cause of cancer-related deaths in adolescents. Emerging evidence indicates that microRNAs (miRNAs) are correlated with clinical and biological characteristics of OS. However, the involvement of miR-199a-5p in OS development remains unclear. In this study, we examined the function of miR-199a-5p in vitro and in vivo. The results showed that miR-199a-5p was significantly up-regulated in OS patient tissues and cells. The inhibition of miR-199a-5p led to a significant decrease in cell proliferation and tumour growth. We further demonstrated that miR-199a-5p could directly bind to the 3′UTRs of the mRNA of both PIAS3 and p27 and mediate a decrease in the protein levels of PIAS3 and p27, thereby stimulating STAT3 activation and cell cycle progression in OS cells. Rescue experiments of PIAS3 and p27 further revealed that PIAS3 and p27 were functional targets of miR-199a-5p. Moreover, enhancing the expressions of both PIAS3 and p27 using miR-199a-5p-targeted inhibitors in an OS xenograft model was shown to be a promising approach for OS clinical therapy. Our findings indicate that the pathway of miR-199a-5p targeting both PIAS3 and p27 is a possible mechanism that contributes to tumour growth in OS.

show abstract

MiR‐329 suppresses osteosarcoma development by downregulating Rab10

Cited by 46 publications

References 17 publications

RAB10 overexpression promotes tumor growth and indicates poor prognosis of hepatocellular carcinoma

RAB10 overexpression promotes tumor growth and indicates poor prognosis of hepatocellular carcinoma

MicroRNA-329 serves a tumor suppressive role in colorectal cancer by directly targeting transforming growth factor beta-1

MicroRNA-199a-5p promotes tumour growth by dual-targeting PIAS3 and p27 in human osteosarcoma

Contact Info

Product

Resources

About