miR-320 regulates tumor angiogenesis driven by vascular endothelial cells in oral cancer by silencing neuropilin 1

Wu, Yi-Ying; Chen, Yuh-Ling; Jao, Yun-Chia; Hsieh, I-Shan; Chang, Kung-Chao; Hong, Tse-Ming

doi:10.1007/s10456-013-9394-1

Cited by 120 publications

(96 citation statements)

References 49 publications

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“…Various miRNA have been implicated in the invasion, metastasis, and maturation of several types of cancer, including RB (7)(8)(9)(10). miR-320 has been demonstrated to be involved in various types of cancer, including colorectal cancer (11), non-small cell lung cancer (12), cervical cancer (13), and oral cancer (14). In a previous miRNA microarray analysis, miR-320 was identified to be associated with the tumorigenesis of RB for the first time, indicating that miR-320 has a role in the regulation of RB development (15).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-320 regulates autophagy in retinoblastoma by targeting hypoxia inducible factor-1α

Liang¹,

Chen²,

Zhu³

2017

Experimental and Therapeutic Medicine

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Abstract. Retinoblastoma (RB) is the most common malignancy in children. Due to refractory mechanisms of chemoresistance and the toxicity of chemotherapies, novel therapies for RB treatment are urgently required. MicroRNA-320 (miR-320) is believed to be associated with the tumorigenesis of RB, although the mechanism remains unclear. Considering the hypoxic intratumoral region, the roles of miR-320 and hypoxia inducible factor-1α (HIF-1α) in the regulation of autophagy were investigated in 30 human RB samples and WERI-RB1 cells. The results demonstrated that HIF-1α was the downstream target of miR-320, and decreased miRNA-320 or HIF-1α lead to the inhibition of autophagy in WERI-RB1 cells. Compared with WERI-RB1 cells that were not transfected, silenced HIF-1α caused a 1.41-fold increase (P<0.01) in p62, a 2.71-fold decrease of Beclin1, and inhibited miRNA-320. Silenced HIF-1α also resulted in 7.29-and 7.43-fold increases in phosphorylated-mechanistic target of rapamycin (mTOR) and mTOR, respectively. In conclusion, the present results suggest that miRNA-320 may regulate the development of autophagy by targeting HIF-1α and autophagy-related proteins in RB under hypoxic conditions.

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Section: Introductionmentioning

confidence: 99%

MicroRNA-320 regulates autophagy in retinoblastoma by targeting hypoxia inducible factor-1α

Liang¹,

Chen²,

Zhu³

2017

Experimental and Therapeutic Medicine

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“…miR-320a regulates glycolysis and represses angiogenic factors, including Flk1, VEGFc, insulin-like growth factor 1, insulin-like growth factor 1 receptor, and fibroblast growth factor (28,29). miR-320a has also been implicated in tumor angiogenesis by silencing NRP1 (30). miR-27b is thought to promote angiogenesis by targeting antiangiogenic genes (31), including the transmembrane protein SEMA6A.…”

Section: Mechanistic Links To Angiogenesismentioning

confidence: 99%

Angiogenic microRNAs Linked to Incidence and Progression of Diabetic Retinopathy in Type 1 Diabetes

et al. 2015

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Circulating microRNAs (miRNAs) have emerged as novel biomarkers of diabetes. The current study focuses on the role of circulating miRNAs in patients with type 1 diabetes and their association with diabetic retinopathy. A total of 29 miRNAs were quantified in serum samples (n = 300) using a nested case-control study design in two prospective cohorts of the DIabetic REtinopathy Candesartan Trial (DIRECT): PROTECT-1 and PREVENT-1. The PREVENT-1 trial included patients without retinopathy at baseline; the PROTECT-1 trial included patients with nonproliferative retinopathy at baseline. Two miRNAs previously implicated in angiogenesis, miR-27b and miR-320a, were associated with incidence and with progression of retinopathy: the odds ratio per SD higher miR-27b was 0.57 (95% CI 0.40, 0.82; P = 0.002) in PREVENT-1, 0.78 (0.57, 1.07; P = 0.124) in PROTECT-1, and 0.67 (0.50, 0.92; P = 0.012) combined. The respective odds ratios for higher miR-320a were 1.57 (1.07, 2.31; P = 0.020), 1.43 (1.05, 1.94; P = 0.021), and 1.48 (1.17, 1.88; P = 0.001). Proteomics analyses in endothelial cells returned the antiangiogenic protein thrombospondin-1 as a common target of both miRNAs. Our study identifies two angiogenic miRNAs, miR-320a and miR-27b, as potential biomarkers for diabetic retinopathy.Recent studies have begun to unveil a powerful and unexpected role of microRNAs (miRNAs) in numerous forms of diseases, providing a unique opportunity to translate this knowledge into the clinical setting in the form of miRNA-based therapeutics and diagnostics (1,2). miRNAs are small noncoding RNAs with cell-type specific expression patterns that orchestrate biological networks by modulating gene expression. miRNAs form base pairs with their target messenger RNAs and mediate gene silencing, often regulating multiple proteins within the same biological pathways. Given their importance in angiogenesis, along with the technical feasibility in manipulating their function in vivo, vascular miRNAs have become central targets for therapeutic manipulation, including diabetes (3).Additionally, miRNAs circulate in blood. We have previously performed the first systematic analysis of circulating miRNAs in a population-based study (the Bruneck Study) and identified distinct miRNA signatures associated with type 2 diabetes (4) and risk of myocardial infarction (5). We have also highlighted their platelet origin (6) and applied concepts of network topology to explore their biomarker potential (7). Exciting opportunities exist to pursue miRNAs as novel biomarkers for risk estimation and patient stratification (8).The current study addresses the role of circulating miRNAs in patients with type 1 diabetes (T1D) and their association with microvascular complications, in particular, diabetic retinopathy. Our aims were threefold: first, to evaluate whether miRNA profiles are independently associated with retinopathy development and progression in diabetes; second, to quantify the incremental discriminatory power of miRNAs over and above traditional risk markers; ...

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“…Furthermore, Wu et al (28) demonstrated that miR-320, which was decreased in oral squamous cell carcinoma tissues and cell lines, suppressed the tumorigenicity of oral squamous cell carcinoma cells in vitro and tumor angiogenesis in vivo, via inhibition of neuropilin 1. In addition, Wu et al (28) reported that miR-320 was downregulated in prostate cancer, and that the upregulation of miR-320 inhibited carcinogenesis and progression of prostate cancer through the downregulation of the Wnt/β-catenin signaling pathway. The overexpression of miR-320 in prostate stem-like tumor-initiating cells marke dly inhibited the stem cell-like properties of prostate cancer cells, including tumorsphere formation, chemoresistance and tumorigenic abilities (29).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-320 suppresses cervical cancer cell viability, migration and invasion via directly targeting FOXM1

Shi

Zhang

2017

Oncology Letters

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Abstract. Cervical cancer is one of the most common types of gynecological cancer worldwide. has been reported to be downregulated in a number of types of human cancer. However, the expression level and functions of miR-320 in cervical cancer remain unknown. In the present study, miR-320 was identified to be markedly downregulated in cervical cancer tissues and cell lines. For the functional studies, miR-320 mimic or miR-320 inhibitor was introduced into cervical cancer cell lines. The effects of miR-320 on cervical cancer cell viability, migration and invasion were evaluated using MTT, migration and invasion assays, respectively. The results of the present study identified that overexpression of miR-320 suppressed the viability, migration and invasion of cervical cancer cells. In contrast, underexpression of miR-320 improved the viability, migration and invasion of cervical cancer cells. Bioinformatics analysis, dual-luciferase reporter assay and western blot analysis were adopted to investigate the underlying molecular mechanism of the suppressive functions of miR-320 in cervical cancer. The results of the present study demonstrated that miR-320 negatively regulated forkhead box M1 (FOXM1) expression by directly targeting the 3' untranslated region of FOXM1. Furthermore, the functions of FOXM1 short interfering RNA were similar to those induced by miR-320 in cervical cancer, identifying FOXM1 as a functional target of miR-320 in cervical cancer. The results of the present study indicated that miR-320 acted as a tumor suppressor in the viability, migration and invasion of cervical cancer through directly targeting FOXM1, suggesting that miR-320 may be a target for the therapeutic treatment of cervical cancer.

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miR-320 regulates tumor angiogenesis driven by vascular endothelial cells in oral cancer by silencing neuropilin 1

Cited by 120 publications

References 49 publications

MicroRNA-320 regulates autophagy in retinoblastoma by targeting hypoxia inducible factor-1α

MicroRNA-320 regulates autophagy in retinoblastoma by targeting hypoxia inducible factor-1α

Angiogenic microRNAs Linked to Incidence and Progression of Diabetic Retinopathy in Type 1 Diabetes

MicroRNA-320 suppresses cervical cancer cell viability, migration and invasion via directly targeting FOXM1

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