miR-3113-5p, miR-223-3p, miR-133a-3p, and miR-499a-5p are sensitive biomarkers to diagnose sudden cardiac death

Yan, Fengping; Chen, Yuanyuan; Ye, Xing; Fu, Zhiqiang; Wang, Yunlong; Zhang, Le; Luo, Xiaoting

doi:10.1186/s13000-021-01127-x

Cited by 18 publications

(13 citation statements)

References 56 publications

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“…Yan et al [ 66 ] investigated, in heart tissue, the role of four miRNAs (miR-133a-3p, miR-223-3p, miR-499a-5p, miR-3113-5p) as potential biomarkers for the diagnosis of sudden cardiac death (SCD). Based on their results, all miRNAs were significantly upregulated in the SCD group, suggesting their use in the post-mortem investigation in cases of SCD.…”

Section: Resultsmentioning

confidence: 99%

miRNA Dysregulation in Cardiovascular Diseases: Current Opinion and Future Perspectives

Sessa

Salerno

Esposito

et al. 2023

IJMS

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MicroRNAs (miRNAs), small noncoding RNAs, are post-transcriptional gene regulators that can promote the degradation or decay of coding mRNAs, regulating protein synthesis. Many experimental studies have contributed to clarifying the functions of several miRNAs involved in regulatory processes at the cardiac level, playing a pivotal role in cardiovascular disease (CVD). This review aims to provide an up-to-date overview, with a focus on the past 5 years, of experimental studies on human samples to present a clear background of the latest advances to summarize the current knowledge and future perspectives. SCOPUS and Web of Science were searched using the following keywords: (miRNA or microRNA) AND (cardiovascular diseases); AND (myocardial infarction); AND (heart damage); AND (heart failure), including studies published from 1 January 2018 to 31 December 2022. After an accurate evaluation, 59 articles were included in the present systematic review. While it is clear that miRNAs are powerful gene regulators, all the underlying mechanisms remain unclear. The need for up-to-date data always justifies the enormous amount of scientific work to increasingly highlight their pathways. Given the importance of CVDs, miRNAs could be important both as diagnostic and therapeutic (theranostic) tools. In this context, the discovery of “TheranoMIRNAs” could be decisive in the near future. The definition of well-setout studies is necessary to provide further evidence in this challenging field.

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Section: Resultsmentioning

confidence: 99%

miRNA Dysregulation in Cardiovascular Diseases: Current Opinion and Future Perspectives

Sessa

Salerno

Esposito

et al. 2023

IJMS

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“…CircRNAs play functional roles in a variety of biological processes, mostly by acting as miRNA sponges to relieve the repression of target mRNAs at the posttranscriptional level (31,33,42) (32,34,43) (32,34,43) (32, 34, 43) (41,42). For instance, circTP63 functions as a ceRNA to promote lung squamous cell carcinoma progression by upregulating FOXM1 (25) .…”

Section: Discussionmentioning

confidence: 99%

EIF4A3-induced Circ_0001187 facilitates AML suppression through promoting ubiquitin-proteasomal degradation of METTL3 and decreasing m6A modification level mediated by miR-499a-5p/RNF113A pathway

Yang

Han

et al. 2022

Preprint

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Aberrant expression of circRNAs has been proven to play a crucial role in the progression of acute myeloid leukemia (AML), but its regulatory mechanism remains unclear. Herein, we identify a novel m6A modification-related circRNA, Circ_0001187, which is downregulated in AML patients, and its low level contributes to a poor prognosis. We find that the expression of Circ_0001187 is regulated by the modification of DNA methylation and histone acetylation, and its biogenesis is meditated by splicing factor EIF4A3. Knockdown of Circ_0001187 significantly promotes the proliferation and inhibits the apoptosis of AML cells in vitro and in vivo, while overexpression of Circ_0001187 exerts the opposite effects. Moreover, we prove that Circ_0001187 enhances METTL3 protein degradation, which further decreases mRNA m6A modification in AML cells. Mechanistically, Circ_0001187 sponges miR-499a-5p to enhance the expression RNF113A. And RNF113A functions as an E3 ubiquitin ligase to mediate METTL3 ubiquitin/proteasome-dependent degradation by K48-linked polyubiquitin chains. Collectively, our findings highlight the potential clinical implications of Circ_0001187 as a key tumor suppressor of AML via the miR-499a-5p/RNF113A/METTL3 pathway.

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“…Body fluid identification has been the main goal of the forensic use of miRNA analysis in order to provide confirming universal analysis of unidentified biological stains received from crime scenes or evidence objects. However, there are other forensic uses for miRNA profiling that have demonstrated value but are mostly unexplored and warrant more research, including the identification of organ tissue and the estimation of donor age [20]. According to some studies, miRNAs are very biostable and readily circulate in mammalian blood.…”

Section: The Use Of Mirnasmentioning

confidence: 99%

Postmortem Genetic Testing in Sudden Unexpected Death: A Narrative Review

Alzahrani¹,

Alswaimil²,

Alammari³

et al. 2023

Cureus

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Sudden unexpected death (SUD) is one of the challenging situations encountered in forensic medicine. As a rule, a comprehensive forensic assessment is performed to identify the cause of death in such cases; however, the absence of findings suggestive of a cause, i.e., a negative autopsy, warrants further investigation such as a molecular autopsy. In this review, we aim to highlight the genetic causes of SUD, tools used in a molecular autopsy, and the role of screening in surviving relatives. As per several guidelines, the most preferred samples for DNA extraction are whole blood and fresh frozen tissues. Furthermore, Sanger sequencing and next-generation sequencing are the technologies that are used for genetic analysis; the latter overcomes the former's drawbacks in terms of cost-effectiveness, time consumption, and the ability to sequence the whole exome. SUD have diverse etiologies; we can generally classify them into cardiac and non-cardiac causes. Regarding cardiac causes, many conditions having an underlying genetic basis are included, such as channelopathies and cardiomyopathies. Regarding non-cardiac causes of SUD, the main etiologies are epilepsy and metabolic disorders. Nevertheless, it has been proposed that there is a genetic overlap between channelopathies, especially long QT syndromes and epilepsy. Additionally, fatty acid oxidation disorders are major metabolic conditions that are caused by certain genetic mutations that can lead to SUD in infancy. Since many SUD causes have an underlying genetic mutation, it is important to understand the genetic variations not only to recognize the cause of death but also to undertake further preventive measures for surviving relatives. In conclusion, a molecular autopsy has a major role in the forensic examination of cases of SUD.

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miR-3113-5p, miR-223-3p, miR-133a-3p, and miR-499a-5p are sensitive biomarkers to diagnose sudden cardiac death

Cited by 18 publications

References 56 publications

miRNA Dysregulation in Cardiovascular Diseases: Current Opinion and Future Perspectives

miRNA Dysregulation in Cardiovascular Diseases: Current Opinion and Future Perspectives

EIF4A3-induced Circ_0001187 facilitates AML suppression through promoting ubiquitin-proteasomal degradation of METTL3 and decreasing m6A modification level mediated by miR-499a-5p/RNF113A pathway

Postmortem Genetic Testing in Sudden Unexpected Death: A Narrative Review

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