mir-300 Promotes Self-Renewal and Inhibits the Differentiation of Glioma Stem-Like Cells

Zhang, Daming; Yang, Guangchao; Chen, Xin; Li, Chunmei; Wang, Lu; Liu, Yaohua; Han, Dayong; Liu, Huailei; Hou, Xu; Zhang, Weiguang; Li, Chenguang; Zhan-qiang, Han; Gao, Xin; Zhao, Shen

doi:10.1007/s12031-014-0230-x

Cited by 31 publications

(27 citation statements)

References 25 publications

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“…The ZEB2/miR-145 double-negative feedback loop is important for the control of EMT and stem cell properties during the bone metastasis of prostate cancer cells (22). miR-300 promotes self-renewal and inhibits the differentiation of glioma stem-like cells (23). Although miR-200b was demonstrated to be a tumor suppressor, its role in stemness properties remains unknown.…”

Section: Discussionmentioning

confidence: 99%

miR-200b inhibits CD133+ glioma cells by targeting the AKT pathway

Liu

Peng

et al. 2017

Oncology Letters

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MicroRNA-200b (miR-200b) is a tumor suppressor in multiple tumor types, including gastric cancer, breast cancer, ovarian cancer and glioma. The biological significance of a known normal and cancer stem cell marker, CD133, remains elusive. The aim of the present study was to identify the function and mechinism of miR-200b in suppressing CD133+ glioma cells. CD133+ glioma cells were sorted by flow cytometry. The expression of miR-200b, Ki67, GAP43, GFAP and CD133 were tested by reverse transcription-quantitative polymerase chain reaction. The binding of miR-200b to prominin 1 (PROM1) was certificated by luciferase reporter assay. Cell proliferation was analyzed by bromodeoxyuridine staining. The protein level of CD133, p-AKT, AKT and Notch1 was detected by western blot analysis. Analysis of glioma samples revealed that CD133 expression is negatively associated with miR-200b. PROM1, which is the gene that codes CD133, was certified to be a target of miR-200b. miR-200b expression inhibited the stemness properties and division of the CD133+ glioma cells. Our results identified a miR-200b/CD133/PI3K/Akt signaling axis, exploring the fundamental role of miR-200b and CD133 in glioma stem cell behavior.

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Section: Discussionmentioning

confidence: 99%

miR-200b inhibits CD133+ glioma cells by targeting the AKT pathway

Liu

Peng

et al. 2017

Oncology Letters

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“…For example, Zhang et al showed that miR-300 was commonly upregulated in glioma tissues, and the expression of miR-300 was higher in glioma stem-like cells (GSLCs) [36]. Moreover, miR-300 enhanced the GSLCs self-renewal and reduced differentiation by inhibiting LZTS2 expression.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-300 inhibited glioblastoma progression through ROCK1

Zhou

et al. 2016

Oncotarget

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Glioblastoma is a common type of brain aggressive tumors and has a poor prognosis. MicroRNAs (miRNAs) are a class of small, endogenous and non-coding RNAs that play crucial roles in cell proliferation, survival and invasion. Deregulated expression of miR-300 has been studied in a lot of cancers. However, the role of miR-300 in glioblastoma is still unknown. In this study, we demonstrated that miR-300 expression was downregulated in glioblastoma tissues compared with the normal tissues. Lower expression level of miR-300 was observed in thirty cases (75 %, 30/40) of glioblastoma samples compared with the normal samples. Moreover, the overall survival of glioblastoma patients with lower miR-300 expression level was shorter than those with higher miR-300 expression level. In addition, miR-300 expression was also downregulated in glioblastoma cell lines. Overexpression of miR-300 inhibited cell proliferation, cell cycle and invasion in glioblastoma cell line U87 and U251. Moreover, we identified ROCK1 as a direct target of miR-300 in U87 and U251 cells. Overexpression of ROCK1 partially rescued the miR-300-mediated cell growth. ROCK1 expression levels in glioblastoma tissues were higher than that in normal tissues. ROCK1 expression levels were higher in thirty-one cases of glioblastoma samples than their normal samples. Furthermore, the expression level ROCK1 was inversely correlated with the expression level of miR-300. Importantly, overexpression of miR-300 suppressed glioblastoma progression in an established xenograft model. In conclusion, we revealed that miR-300 might act as a tumor suppressor gene through inhibiting ROCK1 in glioblastoma.

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“…The aberrant expression of miR-300 is a frequent event in multiple types of solid cancer, suggesting its important roles in carcinogenesis and cancer progression (32–34). Similar to other miRNAs, the expression pattern and functions of miR-300 may be different in various types of cancer, in a tissue-specific manner, dependent on the cellular context (32,33).…”

Section: Discussionmentioning

confidence: 99%

“…Thus, miR-300 could inhibit cell invasion and metastasis by negatively regulating EMT in head and neck squamous cell carcinoma. By contrast, the expression of miR-300 was upregulated in glioma tissues and glioma stem-like cells, and overexpression of miR-300 could promote the self-renewal and cell proliferation of glioma stem-like cells (34). Similarly, the expression of miR-300 was also reported to be elevated in osteosarcoma tissues and cell lines compared with that in adjacent non-tumor bone tissues and osteoblastic cells (32).…”

Section: Discussionmentioning

confidence: 99%

“…First, circulating expression of miR-300 has been described in numerous solid cancers besides osteosarcoma, including glioma and head and neck squamous cell carcinoma (33,34). As a consequence, it may be challenging to differentiate whether circulating miR-300 expression is specifically associated with osteosarcoma itself or is a common phenomenon that manifests during the progression of any cancer as a result of perturbations in the host immune response (35,36).…”

Section: Discussionmentioning

confidence: 99%

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Serum miR-300 as a diagnostic and prognostic biomarker in osteosarcoma

et al. 2016

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In order to determine whether microRNA (miR)-300 is a diagnostic and prognostic biomarker in osteosarcoma, the miR-300 levels in serum of 114 osteosarcoma patients and 114 healthy controls were compared, followed by serum analysis of the differences between the pre-operative and post-operative sera of these osteosarcoma patients. It was observed that the concentration levels of miR-300 in the serum of osteosarcoma patients was significantly higher than those in the serum of healthy controls (P<0.01). Furthermore, the concentration levels of miR-300 in the post-operative serum were significantly reduced when compared with the pre-operative serum levels (P<0.001). High miR-300 levels in serum correlated significantly with clinical stage, distant metastasis and poor survival of osteosarcoma patients. Notably, serum miR-300 was an independent prognostic marker for osteosarcoma. In conclusion, our results suggested that serum miR-300 may be a potential and useful noninvasive biomarker for the early detection of osteosarcoma.

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mir-300 Promotes Self-Renewal and Inhibits the Differentiation of Glioma Stem-Like Cells

Cited by 31 publications

References 25 publications

miR-200b inhibits CD133+ glioma cells by targeting the AKT pathway

miR-200b inhibits CD133+ glioma cells by targeting the AKT pathway

MicroRNA-300 inhibited glioblastoma progression through ROCK1

Serum miR-300 as a diagnostic and prognostic biomarker in osteosarcoma

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