miR-29a promotes scavenger receptor A expression by targeting QKI (quaking) during monocyte-macrophage differentiation

Wang, Shuai; Zan, Jie; Wu, Mengyin; Zhao, Wenting; Li, Zhenwei; Pan, Yanyun; Sun, Zewei; Zhu, Jianhua

doi:10.1016/j.bbrc.2015.05.019

Cited by 15 publications

(12 citation statements)

References 23 publications

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“…We use siRNAs to knockdown these three scavenger receptors and found that SRA and LOX-1 participated in the regulation of miR-29a. Interestingly, our previous study (Wang et al, 2015) demonstrated that miR-29a could upregulate SRA expression, thus we identified a positive feedback between miR-29a and SRA. Considering that the upregulation of SRA plays an important role in the pathogenesis of AS after a stimulation by ox-LDL in macrophages, we implied that miR-29a may be an important potential intervention target in AS.…”

Section: Discussionmentioning

confidence: 61%

“…In addition, miR-29a could promote angiogenesis by targeting HMG box-containing protein-1 and phosphatase gene (Wang et al, 2013;Yang et al, 2013). Our previous study also found that miR-29a promotes SRA expression and lipid uptake during monocyte-macrophage differentiation, thus participate in the process of AS (Wang et al, 2015). Therefore, miR-29a has an important research value in AS.…”

Section: Discussionmentioning

confidence: 82%

“…As shown in Figure C, LOX‐1 and SRA but not CD36 knockdown significantly increased the expression level of miR‐29a induced by ox‐LDL, therefore we thought that LOX‐1 and SRA were involved in the upregulation of miR‐29a in macrophages stimulated by ox‐LDL. Our previous study demonstrated that expression of SRA could be upregulated by miR‐29a during monocyte‐macrophage differentiation (Wang et al, ). In our present study, we found that miR‐29a could upregulate SRA expression in ox‐LDL‐treated macrophages and knock down of miR‐29a could weaken SRA expression (Figure D).…”

Section: Resultsmentioning

confidence: 99%

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ox‐LDL increases microRNA‐29a transcription through upregulating YY1 and STAT1 in macrophages

Jian

Dai

Yao

et al. 2017

Cell Biology International

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Macrophages and oxidized low-density lipoprotein (ox-LDL) have been verified playing vital roles in the pathogenesis of atherosclerosis (AS). Previous studies demonstrated that microRNA-29a (miR-29a) was upregulated in many atherogenic process and cells, thus acting as an important participant in AS. But the detailed regulation mechanism of miR-29a in AS has not been fully understood. In our study, we demonstrated a positive feedback loop of ox-LDL-SRA-miR-29a. Furthermore, we found that YY1 and STAT1 were upregulated in ox-LDL-stimulating macrophages followed by translocation in the nucleus and binding to the transcriptional promoter region of miR-29a, thus leading to the increase of miR-29a expression. In addition, we demonstrated that JAK1/2 signaling was involved in miR-29a upregulation. Finally, we found that miR-29a played important roles in the secretion of proinflammation factors and lipid uptake in macrophages. We uncovered the molecular mechanism and provide novel insights into the function and regulatory network of miR-29a expression regulated by ox-LDL in macrophages.

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Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 82%

Section: Resultsmentioning

confidence: 99%

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ox‐LDL increases microRNA‐29a transcription through upregulating YY1 and STAT1 in macrophages

Jian

Dai

Yao

et al. 2017

Cell Biology International

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“…The usage of miR-29 antagonists may prevent plaque remodelling (Ulrich et al 2016). Furthermore, a basic research demonstrated that the expression of Quaking protein was modulated by miR-29a and that inhibiting the expression of Quaking protein in turn resulted in downregulation of scavenger receptor A and lipid uptake, an important process involved in atherosclerosis (Wang et al 2015). In addition, miR-29a could promote angiogenesis by targeting HMG box-containing protein-1 or phosphatase gene (Wang et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Elevated Plasma miR-29a Levels Are Associated with Increased Carotid Intima-Media Thickness in Atherosclerosis Patients

Liu

Zhong

Huang

2017

Tohoku J. Exp. Med.

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Atherosclerotic cardiovascular diseases, such as coronary heart disease, have become a major public health problem all over the world. MicroRNA-29a (miR-29a) modulates expression levels of collagen, inflammatory reaction and other extracellular matrix mRNAs, while adiponectin (APN), a circulating protein secreted by adipocytes, has anti-inflammatory properties. Both play multifaceted roles in angiogenesis or vascular remodelling. However, little is known about plasma miR-29a and APN levels in patients with atherosclerosis. We therefore investigated the relationship between the plasma levels of miR-29a or APN and carotid intima-media thickness (cIMT) in atherosclerosis patients (n = 85, cIMT ≥ 1.2 mm) and the controls (n = 85, cIMT < 1.2 mm). We found that the atherosclerosis group showed higher miR-29a levels (31.15 ± 3.99 vs. 26.39 ± 1.05 Ct, P < 0.001) and lower APN levels (15.93 ± 4.61 vs. 21.80 ± 7.74 ng/ml, P < 0.001), compared with control group. Thus, increased cIMT was associated with higher plasma miR-29a levels (r = 0.688, P < 0.001) and with lower plasma APN levels (r = −0.494, P < 0.001). Furthermore, multiple logistic regression analysis indicated that higher miR-29a levels (OR: 1.136, 95% CI: 1.042-1.240, P = 0.004) increased the risk for atherosclerosis, whereas higher APN levels appeared to be protective (OR: 0.122, 95% CI: 0.055-0.271, P < 0.001). The present study indicates that elevated miR-29a levels and reduced APN levels are associated with atherosclerosis.Keywords: adiponectin; atherosclerosis; carotid intima-media thickness; microRNA; miR-29a Tohoku J. Exp. Med., 2017 March, 241 (3), 183-188. © 2017 Tohoku University Medical Press IntroductionAtherosclerotic cardiovascular diseases such as coronary heart disease and stroke have become a major public health problem all over the world (Murray et al. 2012). It is necessary to detect atherosclerotic cardiovascular diseases at earlier stages. In recent decades, microRNAs (miRs) are short non-coding RNA able to bind specific sequences on target mRNAs, thereby modulating gene expression (Gaudet and Brisson 2015). They are involved in almost every aspect of cellular or biological processes, and dysregulation of miRs correlated with cardiovascular diseases, such as atherosclerosis ). MiR-29a was one of the members of the miR-29 family (Hysolli et al. 2016). The miR-29 family, modulating mRNA level of collagen, inflammatory reaction and other extracellular matrix genes, play a multifaceted role in tissue remodelling and vessels injury Bretschneider et al. 2016). It was reported that miR-29 were up-regulated in the region of the heart adjacent to a myocardial infarction (MI) (van Rooij et al. 2008). Among other miRs, circulating miR-29a levels may represent one of the new biomarkers which significantly correlate with cardiovascular diseases (Roncarati et al. 2014). In addition, a previous study showed that miR-29b was involved in vascular smooth muscle cell migration and proliferation by mediating an epigenetic mechanism (Chen et al. 2011), which wa...

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“…For example, hsa-miR-92a-3p (homology of bbe-miR-92c-3p) negatively regulates Toll-like receptor (TLR)-triggered inflammatory response in macrophages (Lai et al, 2013). As the human homology of bbe-miR-29a-3p, hsa-miR-29a-3p promotes lipid uptake during monocyte-macrophage differentiation (Wang et al, 2015). And, the hsa-miR-375-3p (homology of bbe-miR-375-3p) has an important role in epithelium immune system by regulating goblet cell differentiation (Biton et al, 2011).…”

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confidence: 99%

Identification of microRNA expression profiles in the gill, intestine and hepatic caecum of Branchiostoma belcheri

et al. 2017

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miR-29a promotes scavenger receptor A expression by targeting QKI (quaking) during monocyte-macrophage differentiation

Cited by 15 publications

References 23 publications

ox‐LDL increases microRNA‐29a transcription through upregulating YY1 and STAT1 in macrophages

ox‐LDL increases microRNA‐29a transcription through upregulating YY1 and STAT1 in macrophages

Elevated Plasma miR-29a Levels Are Associated with Increased Carotid Intima-Media Thickness in Atherosclerosis Patients

Identification of microRNA expression profiles in the gill, intestine and hepatic caecum of Branchiostoma belcheri

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