MiR‐29a‐3p suppresses cell proliferation in laryngocarcinoma by targeting prominin 1

Su, Jili; Lu, Eryong; Lu, Lijuan; Zhang, Chao

doi:10.1002/2211-5463.12199

Cited by 28 publications

(23 citation statements)

References 16 publications

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“…miR-29a-3p can modulate CYP2C19,31 ALDH5A1, and SLC22A732 in human liver cells. Up to now, miR-29a-3p can suppress tumor progression by directly decreasing PROM1,33 IGF1R,28 and Notch2 levels 34. In our study, we identified OTUB2 as a new target of miR-29a-3p in PTC.…”

Section: Discussionmentioning

confidence: 49%

miR-29a-3p inhibits growth, proliferation, and invasion of papillary thyroid carcinoma by suppressing NF-κB signaling via direct targeting of OTUB2

Ma¹,

Sun²

2018

CMAR

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BackgroundAberrant expression of microRNAs (miRNAs) is closely involved in cancer development. Downregulation of miR-29a-3p and its tumor suppressive roles in cancer have been revealed by multiple reporters. However, study of its expression pattern and function in papillary thyroid carcinoma (PTC) is rare.Materials and methodsThe expression of miR-29a-3p in PTC tissues and cells was detected by qPCR. CCK-8, plate clone formation, transwell invasion, Western blot, immunohistochem-istry, and luciferase reporter assays were carried out to identify the target of miR-29a-3p and explore its roles and mechanisms in PTC.ResultsDeregulated miR-29a-3p in PTC tissues and cell lines were revealed by qPCR. Restoring miR-29a-3p expression significantly inhibited growth, proliferation, and invasion of PTC cells demonstrated by CCK-8, plate clone formation, and transwell assays. Luciferase reporter assays showed miR-29a-3p can directly target OTUB2 in PTC cells. Ectopic expression of OTUB2 can antagonize the effects of miR-29a-3p on cell growth, proliferation, and invasion of PTC. Mechanistically, OTUB2 overexpression can activate NF-κB signaling mostly by stabilizing TRAF6. Upregulated OTUB2 expression was observed in PTC tissues via immunohistochemistry analysis. Moreover, OTUB2 showed a positive correlation to metastatic status and showed a negative correlation to the overall survival rate in PTC patients.ConclusionDeregulated miR-29a-3p can promote cell growth, proliferation, and invasion in PTC. OTUB2 is a direct downstream target of miR-29a-3p in PTC, and it mediates the effects of deregulated miR-29a-3p by activating TRAF6-associated NF-κB signaling in PTC.

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Section: Discussionmentioning

confidence: 49%

miR-29a-3p inhibits growth, proliferation, and invasion of papillary thyroid carcinoma by suppressing NF-κB signaling via direct targeting of OTUB2

Ma¹,

Sun²

2018

CMAR

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“…MiR-29a was low expression and inhibited tumorigenesis in multiple cancers, including papillary thyroid carcinoma, colorectal cancer, glioma and pancreatic cancer 22–25. Su et al26 indicated that miR-29a inhibited laryngocarcinoma growth by targeting prominin 1. Consistent with the findings of Zamani,27 we revealed that miR-29a was low expressed in cervical cancer tissues and cell lines, and downregulation of miR-29a was associated with poor outcome of cervical cancer patients.…”

Section: Discussionmentioning

confidence: 99%

<p>MiR-29a function as tumor suppressor in cervical cancer by targeting SIRT1 and predict patient prognosis</p>

Peng

Niu

Wang

et al. 2019

OTT

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IntroductionCervical cancer is the second most frequently malignant tumors in females and metastasis is a challenge of the treatment of cervical cancer. MiR-29a is usually low expressed in several tumors and its functions in cervical cancer remain unclear.Patients and methodsThe quantitative real-time polymerase chain reaction was employed to assess the expression of miR-29a and the Sirtuin-1 (SIRT1). Cell metastatic ability was assessed using Transwell and Western blot assays. The dual-luciferase reporter assay was performed to verify that miR-29a targeted to the 3’-untranslated region (UTR) of SIRT1 mRNA.ResultsMiR-29a was low expressed in cervical cancer and downregulation of miR-29a was associated with poor outcome. MiR-29a regulated the expression of SIRT1 by targeting to its 3’-UTR of mRNA in HeLa cells. SIRT1 was upregulated in cervical cancer tissues and cells in comparison with the non-tumor tissues and normal cells. Upregulation of SIRT1 predicted worse outcome of cervical cancer patients. MiR-29a was participated in the migration, invasion and epithelial–mesenchymal transition (EMT) in cervical cancer through directly targeting to the 3’-UTR of SIRT1 mRNA. SIRT1 reversed partial roles of miR-29a on metastasis in cervical cancer.ConclusionmiR-29a suppressed migration, invasion and EMT by directly targeting to SIRT1 in cervical cancer. The newly identified miR-29a/SIRT1 axis provides novel insight into the pathogenesis of cervical cancer.

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“…Laryngeal cancer is known as the leading cause of death in otolaryngology worldwide, which accounts for 26–30% of head and neck tumours [1]. Almost 95% of the histological pathology of laryngeal cancer is squamous carcinoma, and the five-year survival rate for patients reaches 64%[2]. However, the incidence of laryngeal cancer has been increasing in recent years.…”

Section: Introductionmentioning

confidence: 99%

Knockdown of YAP inhibits growth in Hep-2 laryngeal cancer cells via epithelial-mesenchymal transition and the Wnt/β-catenin pathway

et al. 2019

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Background Yes-associated protein (YAP) plays a crucial role in tumour development and it is the main effector of the Hippo signalling pathway. However, the mechanism underlying YAP downregulation in laryngeal cancer is still unclear. In our previous study, we found that YAP, compared with adjacent tissues, was expressed higher in laryngeal cancer and was also closely associated with histological differentiation, TNM stage and poor prognosis. Methods In this study, we attempted to determine whether silenced YAP could downregulate human laryngeal carcinoma Hep-2 cells progression. YAP was downregulated in Hep-2 cells by shRNA, and the malignant ability of Hep-2 was assessed in vitro and in vivo. Results In vitro, CCK-8, colony formation and wound healing assays showed that downregulation of YAP significantly reduced the rates of proliferation, migration, and invasion in Hep-2 cells. Downregulation of YAP distinctly induced G2/M cycle arrest and increased the rate of apoptosis. Accordingly, western blot assay suggested that the expression of DKK1, vimentin and β-catenin was significantly decreased after YAP downregulated treatment, thereby indicating that YAP mediated the EMT programme and the Wnt/β-catenin signalling pathway in carcinoma of the larynx. Furthermore, silencing of YAP suppressed Hep-2 cell tumourigenesis and metastasis in vivo. Conclusion In summary, our findings demonstrated the proliferation of YAP downregulation and the invasion of Hep-2 cells via downregulating the Wnt/β-catenin pathway in vitro and in vivo, suggesting that YAP may provide a potential therapeutic strategy for the treatment of laryngeal cancer.

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MiR‐29a‐3p suppresses cell proliferation in laryngocarcinoma by targeting prominin 1

Cited by 28 publications

References 16 publications

miR-29a-3p inhibits growth, proliferation, and invasion of papillary thyroid carcinoma by suppressing NF-κB signaling via direct targeting of OTUB2

miR-29a-3p inhibits growth, proliferation, and invasion of papillary thyroid carcinoma by suppressing NF-κB signaling via direct targeting of OTUB2

<p>MiR-29a function as tumor suppressor in cervical cancer by targeting SIRT1 and predict patient prognosis</p>

Knockdown of YAP inhibits growth in Hep-2 laryngeal cancer cells via epithelial-mesenchymal transition and the Wnt/β-catenin pathway

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MiR‐29a‐3p suppresses cell proliferation in laryngocarcinoma by targeting prominin 1

Cited by 28 publications

References 16 publications

miR-29a-3p inhibits growth, proliferation, and invasion of papillary thyroid carcinoma by suppressing NF-&kappa;B signaling via direct targeting of OTUB2

miR-29a-3p inhibits growth, proliferation, and invasion of papillary thyroid carcinoma by suppressing NF-&kappa;B signaling via direct targeting of OTUB2

<p>MiR-29a function as tumor suppressor in cervical cancer by targeting SIRT1 and predict patient prognosis</p>

Knockdown of YAP inhibits growth in Hep-2 laryngeal cancer cells via epithelial-mesenchymal transition and the Wnt/β-catenin pathway

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miR-29a-3p inhibits growth, proliferation, and invasion of papillary thyroid carcinoma by suppressing NF-κB signaling via direct targeting of OTUB2

miR-29a-3p inhibits growth, proliferation, and invasion of papillary thyroid carcinoma by suppressing NF-κB signaling via direct targeting of OTUB2