miR-224 Affects Mammary Epithelial Cell Apoptosis and Triglyceride Production by Downregulating<i>ACADM</i>and<i>ALDH2</i>Genes

Shen, Binglei; Pan, Qiqi; Yang, Yuwei; Gao, Yan; Liu, Xin; Li, Wei; Han, Yunsheng; Xue, Yan; Qu, Yongli; Zhao, Zhihui

doi:10.1089/dna.2016.3540

Cited by 21 publications

(14 citation statements)

References 34 publications

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“…MiR-224 was reported to inhibit cell proliferation and metastasis in NSCLC A549 cell lines. 41 Shen et al 45 reported that miR-224 could downregulate gene expression of ACADM and ALDH2, inhibit the formation of triglyceride and influence lipid metabolism and apoptosis. Therefore, it is hypothesized that the profiles of miR-23a, miR-25, miR-145 and miR-224 might be associated with the inflammation, oxidative stress, immune imbalance, EMT, cell proliferation, apoptosis and lipid metabolism of COPD.…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics-based identification of potential microRNA biomarkers in frequent and non-frequent exacerbators of COPD

Liu

Xue

et al. 2018

COPD

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ObjectivesMicroRNAs (miRNAs) play essential roles in the development of COPD. In this study, we aimed to identify and validate potential miRNA biomarkers in frequent and non-frequent exacerbators of COPD patients using bioinformatic analysis.Materials and methodsThe candidate miRNA biomarkers in COPD were screened from Gene Expression Omnibus (GEO) dataset and identified using GEO2R online tool. Then, we performed bioinformatic analyses including target prediction, gene ontology (GO), pathway enrichment analysis and construction of protein–protein interaction (PPI) network. Furthermore, the expression of the identified miRNAs in peripheral blood monocular cells (PBMCs) of COPD patients was validated using quantitative real-time polymerase chain reaction (qRT-PCR).ResultsMiR-23a, miR-25, miR-145 and miR-224 were identified to be significantly downregulated in COPD patients compared with healthy controls. GO analysis showed the four miRNAs involved in apoptotic, cell differentiation, cell proliferation and innate immune response. Pathway analysis showed that the targets of these miRNAs were associated with p53, TGF-β, Wnt, VEGF and MAPK signal pathway. In healthy controls, the miR-25 and miR-224 levels were significantly decreased in smokers compared with nonsmokers (P<0.001 and P<0.05, respectively). In COPD patients, the levels of miR-23a, miR-25, miR-145 and miR-224 were associated with Global Initiative for Chronic Obstructive Lung Disease (GOLD) stages. Notably, miR-23a and miR-145 were significantly elevated in non-frequent exacerbators compared with frequent exacerbators (P<0.05), and miR-23a showed higher area under the receiver–operator characteristic curve (AUROC) than miR-145 (0.707 vs 0.665, P<0.05).ConclusionMiR-23a, miR-25, miR-145 and miR-224 were associated with the development of COPD, and miR-23a might be a potential biomarker for discriminating the frequent exacerbators from non-frequent exacerbators.

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Section: Discussionmentioning

confidence: 99%

Bioinformatics-based identification of potential microRNA biomarkers in frequent and non-frequent exacerbators of COPD

Liu

Xue

et al. 2018

COPD

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“…Generally, U6 and GADPH are expressed at relatively constant levels in normal and pathological conditions. These genes may be used as housekeeping genes in brain damage ( Chi et al, 2014 ; Alarcon et al, 2015 ; Li G. et al, 2016 ; Shen et al, 2016 ). There are no significant differences in the expression of U6 and GADPH in the gene expression patterns in the cerebral cortex of mice infected with PHEV detected using microarray in our previous study ( Lan et al, 2014 ), so we take them as internal reference genes for the relative quantification of other genes in this study.…”

Section: Methodsmentioning

confidence: 99%

“…The quantification of miRNAs was performed using the Bulge-Loop TM miRNA qRT-PCR Primer Set (RiboBio, China). miR-21a-5p and Caskin1 expressions were determined by RT-PCR using SYBR Green Master Mix kit as described previously ( Shen et al, 2016 ). The relative expression was analyzed using the 2 -ΔΔCT method.…”

Section: Methodsmentioning

confidence: 99%

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miR-21a-5p Contributes to Porcine Hemagglutinating Encephalomyelitis Virus Proliferation via Targeting CASK-Interactive Protein1 In vivo and vitro

et al. 2017

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Porcine hemagglutinating encephalomyelitis virus (PHEV) is a highly neurovirulent coronavirus that can cause nervous symptoms in piglets with muscle tremors, hind limb paralysis, and nystagmus. Whether some factors affect virus replication and proliferation had not been fully understood in the course of nerve damage caused by PHEV infection. In recent years, some reports suggested that miRNA might play a key regulatory role in viral infection. In this study, we found the miR-21a-5p is notably up-regulated in the brains of mice and N2a cells infected with PHEV, and it down-regulated the expression of CASK-interactive protein1 (Caskin1) by directly targeting the 3′-UTR of Caskin1 using a Dual-Luciferase reporter assay. The over-expression of miR-21a-5p or Caskin1 knockdown in the host significantly contributes to PHEV proliferation. Conversely, the silencing of miR-21a-5p by miR-21a-5p inhibitors suppressed the virus proliferation. Taken together, our results indicate that Caskin1 is the direct target gene of miR-21a-5p, and it is advantageous to virus proliferation by down-regulating Caskin1. These findings may help in the development of strategies for therapeutic applications.

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“…Certain miRNAs have been reported to either promote or inhibit triglyceride production and to suppressapoptosis of MECs at growing stages. For instance, miR-224 overexpression could decrease triglyceride synthesis and promote apoptosis 12 . Bta-miR-29b promotes triglyceride production and suppress apoptosis 11 .…”

Section: Introductionmentioning

confidence: 99%

MiR-152 Regulates Apoptosis and Triglyceride Production in MECs via Targeting ACAA2 and HSD17B12 Genes

Yang

Fang

Yang

et al. 2018

Sci Rep

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Mammary epithelial cells (MECs) affect milk production capacity during lactation and are critical for the maintenance of tissue homeostasis. Our previous studies have revealed that the expression of miR-152 was increased significantly in MECs of cows with high milk production. In the present study, bioinformatics analysis identified ACAA2 and HSD17B12 as the potential targets of miR-152, which were further validated by dual-luciferase repoter assay. In addition, the expressions of miR-152 was shown to be negatively correlated with levels of mRNA and protein of ACAA2, HSD17B12 genes by qPCR and western bot analysis. Furthermore, transfection with miR-152 significantly up-regulated triglyceride production, promoted proliferation and inhibited apoptosis in MECs. Furthermore, overexpression of ACAA2 and HSD17B12 could inhibit triglyceride production, cells proliferation and induce apoptosis; but sh234-ACAA2-181/sh234-HSD17B12-474 could reverse the trend. These findings suggested that miR-152 could significantly influence triglyceride production and suppress apoptosis, possibly via the expression of target genes ACAA2 and HSD17B12.

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miR-224 Affects Mammary Epithelial Cell Apoptosis and Triglyceride Production by DownregulatingACADMandALDH2Genes

Cited by 21 publications

References 34 publications

Bioinformatics-based identification of potential microRNA biomarkers in frequent and non-frequent exacerbators of COPD

Bioinformatics-based identification of potential microRNA biomarkers in frequent and non-frequent exacerbators of COPD

miR-21a-5p Contributes to Porcine Hemagglutinating Encephalomyelitis Virus Proliferation via Targeting CASK-Interactive Protein1 In vivo and vitro

MiR-152 Regulates Apoptosis and Triglyceride Production in MECs via Targeting ACAA2 and HSD17B12 Genes

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