MiR‐216a‐3p suppresses the proliferation and invasion of cervical cancer through downregulation of ACTL6A‐mediated YAP signaling

Zhao, Juan; Li, Long; Yang, Ting

doi:10.1002/jcp.29783

Cited by 25 publications

(19 citation statements)

References 39 publications

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“…A recent study reported that miRNAs play important roles in cervical cancer. For example, miR-216a-3p inhibits cervical cancer cell proliferation and invasion by regulating actin-like 6A ( 55 ). Furthermore, miR-195-5p suppresses migration and invasion in cervical cancer by targeting ADP ribosylation factor-like GTPase 2 (ARL2) ( 56 ).…”

Section: Discussionmentioning

confidence: 99%

Long non‑coding RNA UCA1 enhances cervical cancer cell proliferation and invasion by regulating microRNA‑299‑3p expression

Xing

Chen

2021

Oncol Lett

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The long non-coding RNA, urothelial cancer-associated 1 (UCA1) is an important regulator in several tumors. However, to the best of our knowledge, the clinical roles of UCA1 in cervical cancer remain unclear. Thus, the present study aimed to investigate the function and mechanism of UCA1 in cervical cancer. Reverse transcription-quantitative PCR analysis was performed to detect UCA1 and microRNA (miR)-299-3p expression in cervical cancer tissues and cell lines. The Cell Counting Kit-8 and Transwell assays were performed to assess cell proliferation and invasion, respectively. Furthermore, the dual-luciferase reporter assay was performed to confirm the association between UCA1 and miR-299-3p. Rescue experiments were performed to determine the mechanism of the UCA1/miR-299-3p axis. The results demonstrated that UCA1 expression was upregulated in cervical cancer tissues and cell lines. Furthermore, overexpression of UCA1 enhanced the proliferation and invasion of cervical cancer cells, the effects of which were reversed following UCA1 knockdown. Notably, UCA1 interacted with miR-299-3p and negatively regulated miR-299-3p expression. In addition, miR-299-3p expression was downregulated in cervical cancer tissues and cell lines. Overexpression of miR-299-3p suppressed the proliferation and invasion of cervical cancer cells, reversing the effects of UCA1 knockdown on cervical cancer cell proliferation. Taken together, the results of the present study suggest that UCA1 promotes cell proliferation and invasion by regulating miR-299-3p expression in cervical cancer.

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Section: Discussionmentioning

confidence: 99%

Long non‑coding RNA UCA1 enhances cervical cancer cell proliferation and invasion by regulating microRNA‑299‑3p expression

Xing

Chen

2021

Oncol Lett

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“… 7 , 22 Increasing evidence has suggested its involvement with tumorigenesis and development of cancer. 7 ACTL6A has been reported to be overexpressed in a variety of malignancies, including hepatocellular carcinoma, 9 ovarian cancer, 18 cervical cancer, 23 and esophageal squamous cell carcinoma, 11 which is correlated with the prognosis of patients with malignancies. This evidence suggests that ACTL6A is a potential oncogene, and it is observed that ACTL6A expression is also upregulated in PC in our study, which is consistent with previous studies.…”

Section: Discussionmentioning

confidence: 99%

“… 30 Additional evidence suggested that ACTL6A promoted the progression of cervical cancer and laryngeal squamous cell carcinoma through activation of yes-associated protein (YAP) signaling. 23 , 31 Besides, ACTL6A could stabilize transcriptional regulators YAP and transcriptional coactivator with PDZ-binding motif (TAZ) to regulate the proliferation, migration, and invasion of glioma. 32 Further studies revealed that the knockdown of ACTL6A gene resulted in the inhibition of protein kinase B (AKT) signaling pathway to suppress cell migration and increased sensitivity of glioma cells to temozolomide.…”

Section: Discussionmentioning

confidence: 99%

Upregulated Expression of Actin-Like 6A is a Risk Factor Affecting the Prognosis of Pancreatic Cancer

Zhang¹,

Guo²,

Zhang³

2021

CMAR

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Purpose Actin-like 6A (ACTL6A), a regulatory subunit of the ATP-dependent chromatin-remodeling complex SWI/SNF, acts as an oncogenic factor. This study is aimed at evaluating the correlation between ACTL6A expression and clinicopathological parameters in pancreatic cancer (PC) patients. Methods The differences of Actl6a mRNA expression between PC tissues and normal pancreatic tissues were analyzed in public databases, and ACTL6A expression was then determined and confirmed in 60 paired tissue specimens using immunohistochemistry staining. The association analysis between ACTL6A expression and the clinicopathological characteristics was analyzed, as well as Kaplan–Meier survival analysis. Univariate and multivariate Cox analyses were performed to identify the prognostic factors in the overall survival (OS) of patients with PC. Results The mRNA expression of Actl6a showed significantly higher in PC compared to normal controls (p < 0.05) from public databases. The score of immunohistochemistry staining further confirmed that ACTL6A expression was significantly upregulated in PC tissues (p < 0.001) through immunohistochemistry staining. High ACTL6A expression was associated with lymphovascular space invasion of PC. Kaplan–Meier analysis revealed that the high expression of ACTL6A was markedly associated with poor OS. Moreover, univariate and multivariate analysis demonstrated that ACTL6A acted as an independent risk factor for PC prognosis. Conclusion ACTL6A is upregulated in PC and acts as a risk factor for poor prognosis in patients with PC, and therefore clinicians could around it design preventive measures and individualized treatment to improve mortality in patients with PC.

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“…In human cervical cancer, numbers of miRNAs were found to be abnormal expressed during its initiation and progression, including miR-205, miR-210-3p, miR-216a-3p, etc. [11][12][13]. miR-552 is a newly discovered miRNA, its function and mechanism of action in biological processes and diseases are not completely known.…”

Section: Introductionmentioning

confidence: 99%

miR-552 promotes the proliferation and metastasis of cervical cancer cells through targeting MUC15 pathway

Zhang

Dou

2021

J. Cancer

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Accumulating evidence shows that microRNAs (miRNAs) play key roles in tumorigenesis, progression, recurrence and drug resistance of malignant tumors. The tumor-promoting role of miR-552 has been evidenced in multiple tumors. Yet, the relevance of miR-552 in cervical cancer remains undetermined. This study aimed to investigate the role of miR-552 in cervical cancer proliferation and metastasis. Herein, we for first found that miR-552 expression was upregulated in cervical cancer tissues compared with their normal controls. Functional assays revealed that miR-552 promoted the proliferation and metastasis of cervical cancer cells. Mechanically, bioinformatics and luciferase reporter analysis identified MUC15 as a direct target of miR-552. Reduced MUC15 expression was detected in cervical cancer, and MUC15 overexpression exhibited a tumor-suppressive effect. MUC15 restoration partially abolished the discrepancy of growth and metastasis capacity between miR-552 overexpression cervical cancer cells and control cells. Taken together, these data demonstrate that miR-552 acts as a potential oncogene miRNA in cervical cancer, which exerts its function through targeting MUC15.

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MiR‐216a‐3p suppresses the proliferation and invasion of cervical cancer through downregulation of ACTL6A‐mediated YAP signaling

Cited by 25 publications

References 39 publications

Long non‑coding RNA UCA1 enhances cervical cancer cell proliferation and invasion by regulating microRNA‑299‑3p expression

Long non‑coding RNA UCA1 enhances cervical cancer cell proliferation and invasion by regulating microRNA‑299‑3p expression

Upregulated Expression of Actin-Like 6A is a Risk Factor Affecting the Prognosis of Pancreatic Cancer

miR-552 promotes the proliferation and metastasis of cervical cancer cells through targeting MUC15 pathway

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