miR-210 expression is associated with methionine-induced differentiation of trout satellite cells

Abstract

In fish, data on microRNAs (miRNAs) involved in myogenesis are scarce. In order to identify miRNAs involved in satellite cell differentiation, we used a methionine depletion/replenishment protocol to synchronize myogenic cell differentiation. Our results validated that methionine removal (72 h) from the medium strongly decreased and expression, indicating differentiation arrest. In contrast, methionine replenishment rescued expression of and, showing a resumption of differentiation. We performed a miRNA array analysis of myogenic cells under three conditions: presence of methionine for 72 h (control), absence of methionine for 72 h (Meth-) and absence of methionine for 48 h followed by 24 h of methionine replenishment (Meth-/+). A clustering analysis identified three clusters: cluster I corresponds to miRNA upregulated only in Meth-/+ conditions; cluster II corresponds to miRNA downregulated only in Meth-/+ conditions; cluster III corresponds to miRNAs with high expression in control, low expression in Meth- conditions and intermediate expression after methionine replenishment (Meth-/+). Cluster III was very interesting because it fitted with the data obtained for and (supporting an involvement in differentiation) and contained seven miRNAs with muscle-related function (e.g. miR-133a) and one (miR-210) with unknown function. Based on our previously published miRNA repertoire ( Juanchich et al., 2016), we confirmed miR-133a was expressed only in white muscle and showed that miR-210 had strong expression in white muscle. We also showed that miR-210 expression was upregulated during differentiation of satellite cells, suggesting that miR-210 was potentially involved in the differentiation of satellite cells.

Guanidinoacetic Acid Regulates Myogenic Differentiation and Muscle Growth Through miR-133a-3p and miR-1a-3p Co-mediated Akt/mTOR/S6K Signaling Pathway

“…Meanwhile, miRNAs are also known to participate in nutrient-regulated myogenesis. For instance, genistein, which is abundant in soybean, enhances myogenic differentiation by regulating miR-222 expression [ 26 ], methionine induces myogenic differentiation in trout using uses miR-210 [ 27 ], and dietary energy levels affect muscle fiber type transformation by changing miRNA expression [ 28 ].…”

A Novel Mechanism of bta-miR-210 in Bovine Early Intramuscular Adipogenesis

“…The iron-sulfur cluster scaffold homolog and cytochrome c oxidase assembly protein genes, regulators of mitochondrial and tricarboxylic acid cycle function, have been reported as targets of miR-210 [ 38 ]. During the differentiation of satellite cells, the expression of miR-210 was upregulated, indicating the potential involvement of miR-210 in trout myogenesis [ 39 ]. Furthermore, the miR-210 expression level also increased during myogenic differentiation with a Hif1a-dependent mechanism in normoxia.…”

Physiological and Molecular Mechanisms of Methionine Restriction

“…One possible mechanism that has emerged as a factor in MR phenotypes is microRNAs (miRNAs). Studies have shown that MR alters miRNA expression levels in rainbow trout myosatellite cells ( 4 , 5 ) and bone structures of mice ( 6 ), supporting a proposed connection between MR and miRNAs. This review will discuss the history and phenotypes associated with MR, as well as the role for miRNAs in MR phenotypes.…”

“…Recent evidence also supports a role for miRNAs in the MR phenotype in teleosts and mammals. Rainbow trout myosatellite cells exposed to methionine-deficient cell media regulate miRNAs that reduce their capacity for differentiation (miR-133a, miR-206, and miR-210) ( 4 ). Rainbow trout fed an MR (0.775%) diet for 4 weeks had lower levels of miR-133a in the skeletal muscle at 4 weeks and increased glucose tolerance following a glucose challenge at 8 weeks ( 5 ).…”

“…The activation of miRNAs during MR provides a potential link between changes in methylation and the ISRs in cells. Studies utilizing rainbow trout myosatellite cells in vitro and juvenile rainbow trout in vivo ( 4 , 5 ) have shown that methionine can regulate the level of expression of miRNAs in teleosts (Figure 2 ). Studies done in mammalian systems have also shown that miRNAs are differentially regulated in the plasma, liver, and bone marrow of MR mice ( 6 ).…”