A Novel Mechanism of bta-miR-210 in Bovine Early Intramuscular Adipogenesis

Ren, Ling; Li, Qian; Hu, Xin; Yang, Qiyuan; Du, Min; Xing, Yishen; Wang, Yahui; Li, Junya; Zhang, Lupei

doi:10.3390/genes11060601

Cited by 6 publications

(4 citation statements)

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“…Advancements to high-throughput sequencing technology has allowed deeper exploration of cellular regulatory processes (Katz et al, 2010 ). Previous studies have already revealed the regulatory effects of human miRNA, including miR-204 (Zhang Z. et al, 2020 ), miR-27a (Kim et al, 2010 ), miR-210 (Ren et al, 2020 ), miR-24 (Liu et al, 2020 ), miR-378 (Duarte et al, 2020 ), miR-149-5p (Khan et al, 2020 ), miR-143 (Zhang L. et al, 2020 ), and miR-145 (Wang et al, 2020 ), on adipogenesis. These classic mechanisms also occur in the adipose tissue, where they have a large impact on the surrounding tissue (Arcidiacono et al, 2020 ; Raza et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Bovine Pre-adipocyte Adipogenesis Is Regulated by bta-miR-150 Through mTOR Signaling

Chen

Raza

et al. 2021

Front. Genet.

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Micro RNA (miR) are recognized for their important roles in biological processes, particularly in regulatory componentization. Among the miR, miR-150 has been the focus of intense scrutiny, mostly due to its role in malignant tumors. A comparison between steer and bull adipose tissues identified bta-miR-150 as one of the nine downregulated miRNAs, although its function remains unknown (GEO:GSE75063). The present study aimed to further characterize the role of bta-miR-150 in cattle. bta-miR-150 has a negative regulatory effect on the differentiation of bovine adipocytes and promotes proliferation. Overexpression of bta-miR-150 can promote mRNA and protein expression of the marker genes CDK1, CDK2, and PCNA, increase the number of EdU-stained cells, promote adipocyte proliferation, inhibit adipocyte differentiation, and reduce lipid droplet formation. Results of RNA-seq and WGCNA analyses showed that the mammalian target of the rapamycin signaling pathway, which plays a major regulatory role, is dysregulated by the overexpression and inhibition of miR-150. We found that the target gene of bta-miR-150 is AKT1 and that bta-miR-150 affects AKT1 phosphorylation levels. These results showed that bta-miR-150 plays a role in adipogenic differentiation and might therefore have applications in the beef industry.

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Section: Introductionmentioning

confidence: 99%

Bovine Pre-adipocyte Adipogenesis Is Regulated by bta-miR-150 Through mTOR Signaling

Chen

Raza

et al. 2021

Front. Genet.

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“…ese genes play various roles in biological processes in which WISP2, S100A10, and APOC1 indirectly or directly participate in lipid metabolism. WISP2 is a novel adipokine, and it has been reported that WISP2 knockdown enhanced adipogenesis [25]. S100A10 is a cell membrane repair protein holding a complex interaction relationship with the phospholipid membrane [26].…”

Section: Discussionmentioning

confidence: 99%

In Silico Analysis of the Gene Expression Patterns between Aldosterone-Producing Adenoma and Nonfunctional Adrenocortical Adenoma

Dai

Wen

et al. 2021

Genetics Research

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Primary aldosteronism is the most common form of secondary hypertension, and aldosteronoma makes up a significant proportion of primary aldosteronism cases. Aldosteronoma is also called aldosterone-producing adenoma (APA). Although there have been many studies about APA, the pathogenesis of this disease is not yet fully understood. In this study, we aimed to find out the difference of gene expression patterns between APA and nonfunctional adrenocortical adenoma (NFAA) using a weighted gene coexpression network (WGCNA) and differentially expressed gene (DEG) analysis; only the genes that meet the corresponding standards of both methods were defined as real hub genes and then used for further analysis. Twenty-nine real hub genes were found out, most of which were enriched in the phospholipid metabolic process. WISP2, S100A10, SSTR5-AS1, SLC29A1, APOC1, and SLITRK4 are six real hub genes with the same gene expression pattern between the combined and validation datasets, three of which indirectly or directly participate in lipid metabolism including WISP2, S100A10, and APOC1. According to the gene expression pattern of DEGs, we speculated five candidate drugs with potential therapeutic value for APA, one of which is cycloheximide, an inhibitor for phospholipid biosynthesis. All the evidence suggests that phospholipid metabolism may be an important pathophysiological mechanism for APA. Our study provides a new perspective regarding the pathophysiological mechanism of APA and offers some small molecules that may possibly be effective drugs against APA.

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“…For example, miR-425-5p [9] and miR-125a-5p [10] inhibit differentiation in Porcine intramuscular preadipocytes. bta-miR-210 positively regulates the adipogenesis of PDGFRα + cells in Bovine [11]. miR-340-5p inhibits sheep adipocyte differentiation [12].…”

Section: Introductionmentioning

confidence: 99%

LincRNA-MSTRG.673.2 Promotes Chicken Intramuscular Adipocyte Differentiation by Sponging miR- 128-3p

Zhu#,

Zhang,

et al. 2024

Preprint

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Background Intramuscular fat content is positively correlated with meat flavor and juiciness. Increasing the intramuscular fat (IMF) content of chickens while increasing their growth rate has become a hot topic in molecular breeding.The group's previous studies showed that miR-128-3p inhibited chicken intramuscular adipocyte differentiation and lipogenesis. However, the regulatory mechanism of miR-128-3p in intramuscular preadipocytes is currently unknown. In this study, we investigated the mechanism of miR-128-3p regulation of chicken intramuscular adipocyte differentiation and deposition. Methods RNA-seq was performed to screen for long non-coding RNAs (lncRNAs) that bind to miR-128-3p. Dual luciferase reporter system was used to verify the targeting relationship between miR-128-3p and LincRNA-MSTRG.673.2; nucleoplasmic localization analysis and fluorescence in situ hybridization were used to investigate the localization of LincRNA-MSTRG.673.2 in the cells; a series of experiments such as Q-PCR, Oil O Red staining and triglyceride assay were used to explore the effect of interference with LincRNA-MSTRG.673.2 on the differentiation of intramuscular preadipocytes; co-transfection experiments were used to validate the regulatory patterns of miR-128-3p and LincRNA-MSTRG.673.2 in intramuscular adipocytes. Results Transcriptome data analysis of differential LincRNAs indicated that, compared to the NC group, the mimics-treated group had 17 significantly differentially expressed LincRNAs (P < 0.05), including 6 upregulated and 11 downregulated ones; the inhibitor-treated group had 17 differentially expressed LincRNAs (P < 0.05), including 8 upregulated and 9 downregulated ones; and 24 differentially expressed LincRNAs (P < 0.05) were observed when comparing the mimics-treated group to the inhibitor-treated group, with 14 upregulated and 10 downregulated ones. Functional enrichment analysis revealed that DELincRNAs from the overexpression group (M group) and interference group (SI group) were involved in negative regulation of metabolic processes, response to steroid hormones, regulation of actin cytoskeleton. Furthermore, target gene prediction analysis showed that miR-128-3p can target many of the DELincRNAs, such as LincRNA-MSTRG.673.2, LincRNA-MSTRG.39.2, LincRNA-MSTRG.39.3, and LincRNA-MSTRG.14270.2. LincRNA-MSTRG.673.2 was predominantly expressed in cytoplasm of intramuscular adipocytes. Dual luciferase reporter identified the targeting relationship between miR-128-3p and LincRNA-MSTRG.673.2. The results of subsequent functional assays demonstrated that Interfering with MSTRG.673.2 has been shown to inhibit lipid deposition in intramuscular preadipocytes. Transfection experiments have shown that LincR-MSTRG.673.2 can affect the expression of miR-128-3p. Conclusion This study found that LincRNA-MSTRG.673.2 promoted chicken intramuscular adipocytes differentiation by down regulating miR-128-3p. The results are noteworthy for improving chicken meat quality, molecular breeding, and lipid metabolism research.

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A Novel Mechanism of bta-miR-210 in Bovine Early Intramuscular Adipogenesis

Cited by 6 publications

References 50 publications

Bovine Pre-adipocyte Adipogenesis Is Regulated by bta-miR-150 Through mTOR Signaling

Bovine Pre-adipocyte Adipogenesis Is Regulated by bta-miR-150 Through mTOR Signaling

In Silico Analysis of the Gene Expression Patterns between Aldosterone-Producing Adenoma and Nonfunctional Adrenocortical Adenoma

LincRNA-MSTRG.673.2 Promotes Chicken Intramuscular Adipocyte Differentiation by Sponging miR- 128-3p

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