MiR-21-5p, miR-34a, and human telomerase RNA component as surrogate markers for cervical cancer progression

Zhu, Yongqiang; Han, Ying; Tian, Tian; Su, Peihong; Guan, Jian; Chen, Juan; Cao, Yun‐Gui

doi:10.1016/j.prp.2018.01.001

Cited by 35 publications

(21 citation statements)

References 41 publications

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“…MiR-21-5p originates from 5′ end of pre-miR-21 which is mapped at chromosome 17q23.1. MiR-21-5p was confirmed to be highly expressed and plays its oncogene roles in a variety of tumors, including BC 16 – 18 . For example, miR-21-5p advanced migration and invasion of cervical carcinoma cells through targeting von Hippel-Lindau tumor suppressor (VHL) gene 19 .…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA neuroblastoma-associated transcript 1 gene inhibits malignant cellular phenotypes of bladder cancer through miR-21/SOCS6 axis

2018

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Bladder cancer (BC) is one of the most common tumors in the urinary system. Noncoding RNAs are considered to take part in cellular phenotypes and are emerging as diagnostic and prognostic biomarkers of BC. The aim of this study is to investigate the clinical significance of neuroblastoma- associated transcript 1 (NBAT1) gene and its effects on malignant cellular phenotypes in BC. NBAT1 gene was low-expressed in BC tissues and cell lines and its low-expression was related with high pathological grade and metastasis of BC. Upregulation of NBAT1 gene depressed cell viability and invasiveness of KK47 and T24 cells and arrested KK47 and T24 cells at G1 stage. In addition, NBAT1 could target silence the expression of miR-21-5p in RNA-induced silencing complex-dependent manner. KK47 and T24 cells with miR-21-5p knockdown showed reduced cell viability, G1-stage arrest, and depressed invasiveness. MiR-21-5p mediates the regulatory effects of NBAT1 on malignant cellular phenotypes of BC cells. Moreover, SOCS6 gene was a target gene of miR-21-5p, and miR-21-5p modulated malignant cellular phenotypes of KK47 and T24 cells through targeted silencing of SOCS6. In conclusion, low-expression of NBAT1 is associated with the progress and metastasis of BC, and NBAT1 inhibits malignant cellular phenotypes through miR-21-5p/SOCS6 axis in BC. Our findings help to elucidate the tumorigenesis of BC, and future study will provide a novel therapeutic target for BC.

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Section: Introductionmentioning

confidence: 99%

Long noncoding RNA neuroblastoma-associated transcript 1 gene inhibits malignant cellular phenotypes of bladder cancer through miR-21/SOCS6 axis

2018

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“…CC is a common malignant tumor in human beings. 19 In this study, miR-4429 was lowly expressed in CC tissues, and it was related to unfavorable prognosis of patients, and in in vitro experiments, overexpression of miR-4429 or knock down of its target gene FOXM1 could inhibit the proliferation, migration, invasion and EMT progression of underexpressed in gastric cancer, and able to slowdown its progression by targeting methyltransferase-like 3/transporters for protein precursors. 20 Our study showed that the expression of miR-4429 in CC tissues was significantly down-regulated compared with adjacent corresponding normal tissue specimens, which implied that miR-4429 could act as a tumor suppressor in CC.…”

Section: Discussionmentioning

confidence: 68%

“…CC is a common malignant tumor in human beings. 19 In this study, miR-4429 was lowly expressed in CC tissues, and it was related to unfavorable prognosis of patients, and in in vitro experiments, overexpression of miR-4429 or knock down of its target gene FOXM1 could inhibit the proliferation, migration, invasion and EMT progression of CCCs and promote cell apoptosis. In addition, the experimental results of restoration of high-level FOXM1 masked the role of miR-4429-mimics in CCCs in functional analysis.…”

Section: Discussionmentioning

confidence: 69%

<p>miR-4429 Regulates the Proliferation, Migration, Invasion, and Epithelial-Mesenchymal Transition of Cervical Cancer by Targeting FOXM1</p>

Liang¹,

Zheng²,

Wang³

2020

CMAR

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Background: miR-4429 acts as an inhibitor in many malignant tumors and participates in the biological processes of them, but the clinical value and potential molecular mechanism of miR-4429 in cervical cancer (CC) are still under investigation. Objective: To analyze the clinical value and molecular mechanism of miR-4429 in CC. Materials and Methods: A qRT-PCR assay was employed to determine the levels of miR-4429 and forkhead-box M1 (FOXM1) in CC tissues, CC cell lines (SiHa, CaSki, ME-180, and C33A) and human normal immortalized epithelial cell lines (HaCaT). The proliferation, migration, invasion, and apoptosis abilities of ME-180 and C33A cells were detected, and the epithelial-to-mesenchymal transition (EMT)-related proteins in the cells were also determined. Results: MiR-4429 acted as a tumor suppressor gene in CC tissues and cells and was linked to lymph node metastasis and International Federation of Gynecology and Obstetrics (FIGO) staging. The survival analysis revealed that lymph node metastasis, high FIGO staging, and low miR-4429 expression were all related to the unfavorable prognosis of the patients, and the dual-luciferase reporter assay revealed that FOXM1 was the target of miR-4429. Both overexpression of miR-4429 and knock-down of FOXM1 inhibited the proliferation, migration, invasion, and EMT of CCCs, and accelerated the apoptosis of them. Conversely, both knockdown of miR-4429 and overexpression of FOXM1 promoted those biological behaviors of the cells. Moreover, the rescue experiment revealed that the overexpression of FOXM1 reversed the influences of miR-4429 overexpression on the proliferation, migration, invasion, and EMT of CCCs. Conclusion: miR-4429 acts as a tumor suppressor in CC and can directly target FOXM1 to regulate the proliferation, migration, invasion, apoptosis and EMT of CCCs, so miR-4429 is expected to be a new therapeutic target for CC.

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“…Among the up-regulated miRNAs, miR-21 and miR-155 have been widely reported to promote cell migration and invasion (Fig. 1 ), as well as lymph node metastasis in cervical cancer [ 52 , 53 , 66 , 70 , 71 , 75 , 76 , 78 , 79 , 84 - 87 , 90 , 96 - 114 , 117 , 118 , 132 - 134 ]. Additional up-regu-lated miRNAs have been involved in the initiation and progression of cervical carcinogenesis, since the aberrant expression of miR-20b, miR-31, miR-92a, miR-146a, and miR-205 positively correlated with cell proliferation, enhancement of the migration and invasion abilities of cancer cells, and vessel invasion [ 52 , 62 , 64 - 66 , 71 , 72 , 75 , 76 , 79 , 84 , 85 , 89 , 90 , 94 - 96 , 98 , 103 , 117 - 125 , 130 , 131 , 138 , 143 - 145 ].…”

Section: Insights On Mirna and Cervical Cancermentioning

confidence: 99%

“…Different miRNAs are down-regulated as well, such as: miR-34a and miR-214, both promoting cell proliferation, migration, invasion, and angiogenesis [ 53 , 73 , 79 , 86 , 89 , 90 , 98 , 114 , 126 , 132 , 149 , 150 - 154 , 177 - 183 ]; miR-143, involved in the regulation of cell migration and invasion [ 52 , 64 , 69 , 70 , 85 , 89 , 99 , 117 , 148 , 161 - 164 ]; miR-145, whose low expression levels were significantly associated with poor cancer differentiation and lymph node metastasis [ 52 , 64 , 69 , 77 , 84 , 85 , 89 , 118 , 139 , 165 - 169 ]; miR-195, whose reduced expression has been associated to advanced clinical stage in cancer patients and lymph node metastasis [ 52 , 75 , 76 , 78 , 84 , 89 , 110 , 118 , 171 - 175 ]; miR-203, linked to lymph node metastasis [ 53 , 62 - 65 , 75 , 79 , 85 , 86 , 90 , 176 ]; miR-218, associated with cell migration and invasion, induction of epithelial-mesenchymal transition and invasion [ 69 , 71 , 73 , 75 , 76 , 89 , 110 , 118 ,…”

Section: Insights On Mirna and Cervical Cancermentioning

confidence: 99%

Human Papillomavirus Infections, Cervical Cancer and MicroRNAs: An Overview and Implications for Public Health

Sammarco

Tamburro

Pulliero

et al. 2020

MIRNA

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Human Papillomavirus (HPV) is among the most common sexually transmitted infections in both females and males across the world that generally do not cause symptoms and are characterized by high rates of clearance. Persistent infections due at least to twelve well-recognized High-Risk (HR) or oncogenic genotypes, although less frequent, can occur, leading to diseases and malignancies, principally cervical cancer. Three vaccination strategies are currently available for preventing certain HR HPVs-associated diseases, infections due to HPV6 and HPV11 low-risk types, as well as for providing cross-protection against non-vaccine genotypes. Nevertheless, the limited vaccine coverage hampers reducing the burden of HPV-related diseases globally. For HR HPV types, especially HPV16 and HPV18, the E6 and E7 oncoproteins are needed for cancer development. As for other tumors, even in cervical cancer, non-coding microRNAs (miRNAs) are involved in posttranscriptional regulation, resulting in aberrant expression profiles. In this study, we provide a summary of the epidemiological background for HPV occurrence and available immunization programs. In addition, we present an overview of the most relevant evidence of miRNAs deregulation in cervical cancer, underlining that targeting these biomolecules could lead to wide translational perspectives, allowing better diagnosis, prognosis and therapeutics, and with valuable applications in the field of prevention. The literature on this topic is rapidly growing, but advanced investigations are required to achieve more consistent findings on the up-regulated and down-regulated miRNAs in cervical carcinogenesis. Because the expression of miRNAs is heterogeneously reported, it may be valuable to assess factors and risks related to individual susceptibility.

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MiR-21-5p, miR-34a, and human telomerase RNA component as surrogate markers for cervical cancer progression

Cited by 35 publications

References 41 publications

Long noncoding RNA neuroblastoma-associated transcript 1 gene inhibits malignant cellular phenotypes of bladder cancer through miR-21/SOCS6 axis

Long noncoding RNA neuroblastoma-associated transcript 1 gene inhibits malignant cellular phenotypes of bladder cancer through miR-21/SOCS6 axis

<p>miR-4429 Regulates the Proliferation, Migration, Invasion, and Epithelial-Mesenchymal Transition of Cervical Cancer by Targeting FOXM1</p>

Human Papillomavirus Infections, Cervical Cancer and MicroRNAs: An Overview and Implications for Public Health

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