miR-203a-3p-DNMT3B feedback loop facilitates non-small cell lung cancer progression

Yang, Pingshan; Zhang, Dongdong; Zhou, Fang; Chen, Wenyou; Hu, Chuang; Duqing, Xiao; Cai, Sanjun

doi:10.1007/s13577-022-00728-y

Cited by 5 publications

(4 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, chondrocyte apoptosis and cartilage matrix damage can form a vicious cycle, exacerbating each other and disrupting cartilage homeostasis [40,41]. MiR-203a-3p has previously been reported to mediate cell apoptosis in various disease models [36,42,43]. Our study yielded similar results, demonstrating that miR-203a-3p overexpression exerts anti-apoptotic effects by increasing Bcl-2 expression and decreasing Bax and cleaved caspase-3 levels.…”

Section: Discussionsupporting

confidence: 81%

MiR-203a-3p attenuates apoptosis and pyroptosis of chondrocytes by regulating the MYD88/NF-κB pathway to alleviate osteoarthritis progression

Chen,

Liu,

Sun

et al. 2023

Aging

View full text Add to dashboard Cite

Background: Osteoarthritis (OA) is a degenerative joint disease that imposes a significant socioeconomic burden worldwide. Our previous studies revealed a down-regulation of miR-203a-3p in the knee tissues of OA patients. However, the underlying mechanism through which miR-203a-3p mediates the pathological process of OA remains unknown. Thus, we aimed to determine the effects of miR-203a-3p in the progression of OA. Methods: Rat primary chondrocytes were stimulated with 10 μg/mL lipopolysaccharide (LPS) for 24 hours, followed by transfection with 50 nM miR-203a-3p mimic, inhibitor, and siRNA for MYD88 or consistent negative controls for 48 hours. To evaluate the effects of miR-203a-3p on cartilage matrix degradation, oxidative stress, apoptosis, and pyroptosis in chondrocytes, various techniques such as immunofluorescence staining, biochemical analysis, Western blotting, and the TUNEL staining were utilized. In the rat OA model, all rats were randomly divided into four groups: Sham, OA, OA+Agomir negative control (NC), and OA+Agomir. They received intra-articular injections of 25 nmol miR-203a-3p agomir, agomir NC, or normal saline twice a week for the duration of 8 weeks after OA induction. Immunofluorescence staining was performed to evaluate the effects of miR-203a-3p on cartilage matrix degradation in rats. Results: MiR-203a-3p was down-regulated in LPS-treated rat chondrocytes and OA cartilage, and directly targeted MYD88. Moreover, miR-203a-3p significantly inhibited LPS-induced cartilage matrix degradation, oxidative stress, apoptosis, and pyroptosis of chondrocytes via targeting MYD88. Mechanistically, miR-203a-3p exerted protective effects via the inhibition of the MYD88/NF-κB pathway. In the rat OA model, intra-articular injections of miR-203a-3p agomir also significantly inhibited cartilage matrix degradation, thereby alleviating OA progression. Furthermore, the miR-203a-3p agomir-treated arthritic rat dramatically exhibited better articular tissue morphology and lower OARSI scores. Conclusions: MiR-203a-3p plays a role in alleviating the progression of OA by regulating the MYD88/NF-κB pathway, thereby inhibiting cartilage matrix degradation, oxidative stress, apoptosis, and pyroptosis of chondrocytes. It highlights the potential significance of miR-203a-3p as an important regulator of OA.

show abstract

Section: Discussionsupporting

confidence: 81%

MiR-203a-3p attenuates apoptosis and pyroptosis of chondrocytes by regulating the MYD88/NF-κB pathway to alleviate osteoarthritis progression

Chen,

Liu,

Sun

et al. 2023

Aging

View full text Add to dashboard Cite

show abstract

“…Notably, miR-203a-3p and DNMT3B showed feedback regulation that promotes NSCLC advancement. 102 The SOX9/miR-203a axis has recently been implicated in the progression of several cancers. One group reported that with the upregulation of SOX9, miR-203a was frequently downregulated in ESCC tissues in comparison to adjacent normal tissues and SOX9 knockdown or miR-203a overexpression suppresses cell growth, migration, and invasion in vitro, and tumor development in vivo.…”

Section: Methylation Modifications and Transcription Factors Regulati...mentioning

confidence: 99%

“…These findings demonstrated that promoter hypermethylation was a plausible mechanism underlying the decreased expression of miR‐203a‐3p in NSCLC. Notably, miR‐203a‐3p and DNMT3B showed feedback regulation that promotes NSCLC advancement 102 …”

Section: Upstream Regulatory Mechanisms Modulating Mir‐203amentioning

confidence: 99%

miR‐203a—A multifaceted regulator modulating cancer hallmarks and therapy response

Biswal,

Lalruatfela,

Behera

et al. 2023

IUBMB Life

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are a class of noncoding RNAs of about 19–25 nucleotides, which serve as critical modulators of various cellular and biological processes by target gene regulation. Dysregulated expression of miRNAs modulates the pathophysiology of various human diseases, including cancer. Among miRNAs, miR‐203a is one of the most extensively researched dysregulated miRNAs in different cancers. Our review investigated the roles of miR‐203a in the hallmarks of cancer modulating different pathways through target gene regulations, chemoresistance, its crosstalk with other ncRNAs or genes in terms of ceRNAs impacting oncogenesis, and its potential applications in the diagnosis, prognosis, and chemotherapeutic responses in different cancer types. miR‐203a impacts cancer cell behavior by regulating these exclusive hallmarks‐ sustaining proliferation, cell growth, invasion and metastasis, cell death, and angiogenesis. Besides, miR‐203a is found in human circulating biofluids like plasma or serum of colorectal cancer, cervical cancer, and hepatocellular carcinoma, hinting at its potential as a biomarker. Further, miR‐203a is involved in enhancing the chemosensitivity of cisplatin, docetaxel, paclitaxel, doxorubicin, and 5‐fluorouracil in a variety of malignancies through their cognate target genes. These results suggest that miR‐203a is a crucial multifaceted miRNA that controls cancer cell proliferation, metastasis, and chemotherapy response, shedding new light on its possible application.

show abstract

“…Subsequently, they provided conclusive evidence that the miR-203a-3p upregulation leads to a reduction in tumor growth in an in vivo setting. Thereby, miR-203a-3p-DNMT3B feedback loop plays a prominent role in facilitating the progression of NSCLC [ 46 ].…”

Section: Introductionmentioning

confidence: 99%

Non-coding RNAs/DNMT3B axis in human cancers: from pathogenesis to clinical significance

Huang,

Azizi,

Vazirzadeh

et al. 2023

J Transl Med

View full text Add to dashboard Cite

Cancer is a complex disease with many contributing factors, and researchers have gained extensive knowledge that has helped them understand the diverse and varied nature of cancer. The altered patterns of DNA methylation found in numerous types of cancer imply that they may play a part in the disease’s progression. The human cancer condition involves dysregulation of the DNA methyltransferase 3 beta (DNMT3B) gene, a prominent de novo DNA methyltransferase, and its abnormal behavior serves as an indicator for tumor prognosis and staging. The expression of non-coding RNAs (ncRNAs), which include microRNAs (miRNA), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs), is critical in controlling targeted gene expression and protein translation and their dysregulation correlates with the onset of tumors. NcRNAs dysregulation of is a critical factor that influences the modulation of several cellular characteristics in cancerous cells. These characteristics include but are not limited to, drug responsiveness, angiogenesis, metastasis, apoptosis, proliferation, and properties of tumor stem cell. The reciprocal regulation of ncRNAs and DNMT3B can act in synergy to influence the destiny of tumor cells. Thus, a critical avenue for advancing cancer prevention and treatment is an inquiry into the interplay between DNMT3B and ncRNAs. In this review, we present a comprehensive overview of the ncRNAs/DNMT3B axis in cancer pathogenesis. This brings about valuable insights into the intricate mechanisms of tumorigenesis and provides a foundation for developing effective therapeutic interventions.

show abstract

miR-203a-3p-DNMT3B feedback loop facilitates non-small cell lung cancer progression

Cited by 5 publications

References 33 publications

MiR-203a-3p attenuates apoptosis and pyroptosis of chondrocytes by regulating the MYD88/NF-κB pathway to alleviate osteoarthritis progression

MiR-203a-3p attenuates apoptosis and pyroptosis of chondrocytes by regulating the MYD88/NF-κB pathway to alleviate osteoarthritis progression

miR‐203a—A multifaceted regulator modulating cancer hallmarks and therapy response

Non-coding RNAs/DNMT3B axis in human cancers: from pathogenesis to clinical significance

Contact Info

Product

Resources

About