miR-194-5p/BCLAF1 deregulation in AML tumorigenesis

Dell’Aversana, Carmela; Giorgio, Cristina Di; D'Amato, L; Lania, Gabriella; Matarese, Filomena; Saeed, Sadia; Costanzo, Antonella Di; Petrizzi, Valeria Belsito; Ingenito, Concetta; Martens, Joost H.A.; Pallavicini, Isabella; Minucci, Saverio; Carissimo, Annamaria; Stunnenberg, Hendrik G.; Altucci, Lucia

doi:10.1038/leu.2017.64

Cited by 70 publications

(60 citation statements)

References 50 publications

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“…[13][14][15] Decreased expression of miR-194-5p has been reported in various types of malignancy, including glioma, hepatoma carcinoma, gallbladder carcinoma and acute myeloid leukaemia. [16][17][18][19] Overexpressed miR-194-5p in non-small-cell lung cancer suppresses cell migration, invasion and metastasis. 20 These reports indicate that miR-194-5p functions as a tumour suppressor in various human malignancies.…”

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confidence: 99%

Circular RNA USP1 regulates the permeability of blood‐tumour barrier via miR‐194‐5p/FLI1 axis

Gao

et al. 2019

J Cellular Molecular Medi

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Recent studies indicate circular RNAs are related to dysregulation of vascular endothelial cell function, yet the underlying mechanisms have remained elusive. Here, we characterized the functional role of circular RNA USP1 (circ‐USP1) in the regulation of the blood‐tumour barrier (BTB) permeability and the potential mechanisms. In the current study, the circ‐USP1 expressing level was up‐regulated in glioma cerebral microvascular endothelial cells (GECs) of the BTB model in vitro. Knockdown of circ‐USP1 disrupted the barrier integrity, increased its permeability as well as reduced tight junction‐related protein claudin‐5, occludin and ZO‐1 expressions in GECs. Bioinformatic prediction and luciferase assay indicated that circ‐USP1 bound to miR‐194‐5p and suppressed its activity. MiR‐194‐5p contributed to circ‐USP1 knockdown‐induced increase of BTB permeability via targeting and down‐regulating transcription factor FLI1. Furthermore, FLI1 regulated the expressions of claudin‐5, occludin and ZO‐1 in GECs through binding to their promoter regions. Single or combined treatment of circ‐USP1 and miR‐194‐5p effectively promoted anti‐tumour drug doxorubicin across BTB to induce apoptosis of glioma cells. Overall, this present study identified the crucial regulation of circ‐USP1 on BTB permeability via miR‐194‐5p/FLI1 axis‐mediated regulation of tight junction proteins, which might facilitate the development of therapeutics against human gliomas.

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confidence: 99%

Circular RNA USP1 regulates the permeability of blood‐tumour barrier via miR‐194‐5p/FLI1 axis

Gao

et al. 2019

J Cellular Molecular Medi

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“…While some of the miRNAs work as oncogenes, others work as tumor suppressors [120]. For instance, it has been shown that the balance between miR-194-5p and its target BCL2-associated transcription factor 1 (BCLAF1) is commonly deregulated in AML patients [18]. Also, miR-10a-5p was found to be overexpressed in relapsed AML cases [121].…”

Section: Micro Rnasmentioning

confidence: 99%

Role of non-coding RNA networks in leukemia progression, metastasis and drug resistance

et al. 2020

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Early-stage detection of leukemia is a critical determinant for successful treatment of the disease and can increase the survival rate of leukemia patients. The factors limiting the current screening approaches to leukemia include low sensitivity and specificity, high costs, and a low participation rate. An approach based on novel and innovative biomarkers with high accuracy from peripheral blood offers a comfortable and appealing alternative to patients, potentially leading to a higher participation rate. Recently, non-coding RNAs due to their involvement in vital oncogenic processes such as differentiation, proliferation, migration, angiogenesis and apoptosis have attracted much attention as potential diagnostic and prognostic biomarkers in leukemia. Emerging lines of evidence have shown that the mutational spectrum and dysregulated expression of non-coding RNA genes are closely associated with the development and progression of various cancers, including leukemia. In this review, we highlight the expression and functional roles of different types of non-coding RNAs in leukemia and discuss their potential clinical applications as diagnostic or prognostic biomarkers and therapeutic targets.

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“…miR-194-5p was reported to be down-regulated in hypopharyngeal carcinoma, acute myeloid leukemia, bladder cancer and glioblastoma and functioned as a tumor suppressor. [25][26][27] Interestingly, Yang et al found that miR-194-5p was up-regulated in breast cancer tissues and silence of miR-194-5p inhibited cell proliferation and metastasis via targeting SOX17. 28 Meanwhile, decreased miR-194-5p was implicated in sunitinib resistance in human renal cell carcinoma and paclitaxel resistance in ovarian cancer.…”

Section: Discussionmentioning

confidence: 99%

<p>LncRNA PVT1 Enhances Proliferation and Cisplatin Resistance via Regulating miR-194-5p/HIF1a Axis in Oral Squamous Cell Carcinoma</p>

Wang

et al. 2020

OTT

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Background: Oral squamous cell carcinoma (OSCC) is the most frequent oral malignancy. Recent studies have revealed that long non-coding RNA (lncRNA) PVT1 plays important roles in the pathogenesis of various cancers. However, the functional roles of PVT1 in OSCC progression and cisplatin resistance have not been elucidated. Material and Methods: In this study, PVT1 expression level in cisplatin-sensitive and cisplatin-resistant OSCC tissues and cell lines was determined using qRT-PCR. Gain-offunction and loss-of-function assays were performed to explore the biological roles of PVT1 in OSCC cell proliferation and cisplatin resistance. Western blot, luciferase reporter assay and bioinformatics analysis were employed to investigate the underlying mechanism of PVT1 in OSCC progression. Results: Here, we found that PVT1 was frequently up-regulated in cisplatin-resistant tissues and cell lines and strongly correlated with worse overall survival. Functional studies showed that PVT1 promoted OSCC cell proliferation and cisplatin resistance. Mechanistic investigation revealed that PVT1 could positively regulate HIF1a expression via its competing endogenous RNA (ceRNA) activity on miR-194-5p. In addition, miR-194-5p conversely correlated with PVT1 and HIF1a expression in OSCC samples. More importantly, HIF1a knock-down or miR-194-5p overexpression reversed PVT1-induced promotion of OSCC cell proliferation and cisplatin resistance. Conclusion: Our results indicated that PVT1 functions as an oncogene involved in OSCC cell proliferation and cisplatin-resistance and may serve as a novel therapeutic target for OSCC treatment.

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miR-194-5p/BCLAF1 deregulation in AML tumorigenesis

Cited by 70 publications

References 50 publications

Circular RNA USP1 regulates the permeability of blood‐tumour barrier via miR‐194‐5p/FLI1 axis

Circular RNA USP1 regulates the permeability of blood‐tumour barrier via miR‐194‐5p/FLI1 axis

Role of non-coding RNA networks in leukemia progression, metastasis and drug resistance

<p>LncRNA PVT1 Enhances Proliferation and Cisplatin Resistance via Regulating miR-194-5p/HIF1a Axis in Oral Squamous Cell Carcinoma</p>

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