miR-185-5p alleviates CCI-induced neuropathic pain by repressing NLRP3 inflammasome through dual targeting MyD88 and CXCR4

Huang, Airu; Ji, Ling; Huang, Yilong; Q, Yu; Li, Yufeng

doi:10.1016/j.intimp.2021.108508

Cited by 13 publications

(8 citation statements)

References 37 publications

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“… 24 It has been reported that high expression of miR-185-5p can reduce mechanical pain and thermal hyperalgesia in rats with contractile injury, inhibit the aggregation of microglia and astrocytes, and reduce the content of IL-1β, IL-6 and TNF-α, thereby reducing neuropathic pain induced by chronic contractile injury. 32 The mechanism may be exerted by targeting MyD88 and CXCR4. 32 However, miRNA-185-5p might reduce the inflammation of diabetic wound and human skin fibroblasts under AGEs (200 ug/mL) by inhibiting MyD88 and CXCR4.…”

Section: Discussionmentioning

confidence: 99%

Accelerated Wound Healing in Diabetic Rat by miRNA-185-5p and Its Anti-Inflammatory Activity

Wang¹,

Zhao²,

Zheng³

et al. 2023

DMSO

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Aim Addressing both inflammation and epithelialization during the treatment of diabetic foot ulcers is an important step, but current treatment options are limited. MiRNA has important prospects in the treatment of diabetic foot refractory wound ulcers. Previous studies have reported that miR-185-5p reduces hepatic glycogen production and fasting blood glucose levels. We herein hypothesized that miR-185-5p might play an important role in the field of diabetic foot wounds. Materials and Methods MiR-185-5p in skin tissue samples from patients with diabetic ulcers and diabetic rats were measured using quantitative real-time PCR (qRT-PCR). The streptozotocin-induced diabetes rat model (male Sprague-Dawley rats) for diabetic wound healing was conducted. The therapeutic potential was observed by subcutaneous injection of miR-185-5p mimic into diabetic rat wounds. The anti-inflammation roles of miR-185-5p on human dermal fibroblast cells were analyzed. Results We found that miR-185-5p is significantly downregulated in diabetic skin (people with DFU and diabetic rats) compared to controls. Further, in vitro upregulation of miR-185-5p decreased the inflammatory factors (IL-6, TNF-α) and intercellular adhesion molecule 1 (ICAM-1) of human skin fibroblasts under advanced glycation end products (AGEs). Meanwhile, the increase of miR-185-5p promoted cell migration. Our results also confirmed that the topical increase of miR-185-5p decreases diabetic wound p-nuclear factor-κB (p-NF-κB), ICAM-1, IL-6, TNF-α, and CD68 expression in diabetic wounds. MiR-185-5p overexpression boosted re-epithelization and expedited wound closure of diabetic rats. Conclusion MiR-185-5p accelerated wound healing of diabetic rats, reepithelization, and inhibited the inflammation of diabetic wounds in the healing process, a potentially new and valid treatment for refractory diabetic foot ulcers.

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Section: Discussionmentioning

confidence: 99%

Accelerated Wound Healing in Diabetic Rat by miRNA-185-5p and Its Anti-Inflammatory Activity

Wang¹,

Zhao²,

Zheng³

et al. 2023

DMSO

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“…These data are in agreement with other animal findings in which miR-185-5p suppressed autophagy and apoptosis of nucleus pulposus cells in IDD via inhibition of the Wnt/β-catenin signaling pathway [ 120 ]. Furthermore, anti-inflammatory effects have been observed for miR-185-5p in neuropathic pain induced by experimental chronic constrictive injury, where miR-185-5p was reduced in spinal cord tissues and its upregulation attenuated mechanical and thermal hyperalgesia and decreased microglia and astrocytes recruitment and the levels of inflammatory cytokines by repressing NLRP3 inflammasome [ 121 ].…”

Section: Discussionmentioning

confidence: 99%

Expression and Biological Functions of miRNAs in Chronic Pain: A Review on Human Studies

Sabina

Panìco

Mincarone

et al. 2022

IJMS

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Chronic pain is a major public health problem and an economic burden worldwide. However, its underlying pathological mechanisms remain unclear. MicroRNAs (miRNAs) are a class of small noncoding RNAs that post-transcriptionally regulate gene expression and serve key roles in physiological and pathological processes. This review aims to synthesize the human studies examining miRNA expression in the pathogenesis of chronic primary pain and chronic secondary pain. Additionally, to understand the potential pathophysiological impact of miRNAs in these conditions, an in silico analysis was performed to reveal the target genes and pathways involved in primary and secondary pain and their differential regulation in the different types of chronic pain. The findings, methodological issues and challenges of miRNA research in the pathophysiology of chronic pain are discussed. The available evidence suggests the potential role of miRNA in disease pathogenesis and possibly the pain process, eventually enabling this role to be exploited for pain monitoring and management.

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“…The question of whether regional intradermal glucose injections lead to increased ATP production and subsequent regeneration in peripheral sensory neurons is still subject to scientific debate. More research in this field may show that glucose also acts through miR-186-5p in neuropathic pain [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

Isotonic Glucose Injections for Postherpetic Neuralgia in the Elderly

Kersschot¹,

Karavani²

2022

Cureus

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Postherpetic neuralgia (PHN) is a painful condition which is difficult to treat, especially among the elderly. This clinical case describes the treatment of an 88-year-old patient with PHN who continued to suffer from pain for several months despite oral and transdermal pain treatment. Multiple intradermal glucose 5% injections allowed her to discontinue her pain medication regimen after four sessions. The improvement was sustained at the four-month follow-up after the last procedure. A fifth session was performed because of a flaring up of the pain.

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miR-185-5p alleviates CCI-induced neuropathic pain by repressing NLRP3 inflammasome through dual targeting MyD88 and CXCR4

Cited by 13 publications

References 37 publications

Accelerated Wound Healing in Diabetic Rat by miRNA-185-5p and Its Anti-Inflammatory Activity

Accelerated Wound Healing in Diabetic Rat by miRNA-185-5p and Its Anti-Inflammatory Activity

Expression and Biological Functions of miRNAs in Chronic Pain: A Review on Human Studies

Isotonic Glucose Injections for Postherpetic Neuralgia in the Elderly

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