MiR-155 Inhibitor-Laden Exosomes Reverse Resistance to Cisplatin in a 3D Tumor Spheroid and Xenograft Model of Oral Cancer

Sayyed, Adil Ali; Gondaliya, Piyush; Mali, Mukund; Pawar, Abhijeet; Bhat, Palak; Khairnar, Amit; Arya, Neha; Kalia, Kiran

doi:10.1021/acs.molpharmaceut.1c00213

Cited by 42 publications

(35 citation statements)

References 77 publications

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“…The loading of the miRNA into exosomes was enhanced using this TAT-TAR interaction [ 42 , 43 ]. The modified calcium chloride (CaCl 2 ) method (CaCl 2 -heat shock) has successfully loaded nucleic acids into exosomes through forming CaCl 2 -nucleic acid complex, which was absorbed by exosomes under heat shock at 42 °C [ 44 – 46 ]. In addition, plasmid-mediated therapeutic molecule transfer has been gradually applied.…”

Section: The Approaches and Advances In Exosome-mediated Deliverymentioning

confidence: 99%

Recent advances in exosome-mediated nucleic acid delivery for cancer therapy

et al. 2022

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Cancer is a leading public health problem worldwide. Its treatment remains a daunting challenge, although significant progress has been made in existing treatments in recent years. A large concern is the poor therapeutic effect due to lack of specificity and low bioavailability. Gene therapy has recently emerged as a powerful tool for cancer therapy. However, delivery methods limit its therapeutic effects. Exosomes, a subset of extracellular vesicles secreted by most cells, have the characteristics of good biocompatibility, low toxicity and immunogenicity, and great designability. In the past decades, as therapeutic carriers and diagnostic markers, they have caught extensive attention. This review introduced the characteristics of exosomes, and focused on their applications as delivery carriers in DNA, messenger RNA (mRNA), microRNA (miRNA), small interfering RNA (siRNA), circular RNA (circRNA) and other nucleic acids. Meanwhile, their application in cancer therapy and exosome-based clinical trials were presented and discussed. Through systematic summarization and analysis, the recent advances and current challenges of exosome-mediated nucleic acid delivery for cancer therapy are introduced, which will provide a theoretical basis for the development of nucleic acid drugs. Graphical Abstract

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Section: The Approaches and Advances In Exosome-mediated Deliverymentioning

confidence: 99%

Recent advances in exosome-mediated nucleic acid delivery for cancer therapy

et al. 2022

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“…miR-155 is overexpressed in HypoExos from glioma [ 219 ] and hepatocellular carcinoma [ 19 ] and hypoxic TAMs [ 220 ] and is associated with cisplatin resistance [ 221 ]. miR-155 inhibitor-laden exosomes can reverse cisplatin resistance by suppressing drug efflux transporter protein expression in cisplatin-resistant OSCC [ 221 ], but whether HypoExo miR-155 can induce drug resistance by upregulating drug efflux is unknown. Single pathways are also utilized by HypoExos to promote drug resistance.…”

Section: Exosome-mediated Biofunctions In Cancer Under Hypoxiamentioning

confidence: 99%

Exosomes in the hypoxic TME: from release, uptake and biofunctions to clinical applications

Peng

Wei

et al. 2022

Mol Cancer

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Hypoxia is a remarkable trait of the tumor microenvironment (TME). When facing selective pressure, tumor cells show various adaptive characteristics, such as changes in the expression of cancer hallmarks (increased proliferation, suppressed apoptosis, immune evasion, and so on) and more frequent cell communication. Because of the adaptation of cancer cells to hypoxia, exploring the association between cell communication mediators and hypoxia has become increasingly important. Exosomes are important information carriers in cell-to-cell communication. Abundant evidence has proven that hypoxia effects in the TME are mediated by exosomes, with the occasional formation of feedback loops. In this review, we equally focus on the biogenesis and heterogeneity of cancer-derived exosomes and their functions under hypoxia and describe the known and potential mechanism ascribed to exosomes and hypoxia. Notably, we call attention to the size change of hypoxic cancer cell-derived exosomes, a characteristic long neglected, and propose some possible effects of this size change. Finally, jointly considering recent developments in the understanding of exosomes and tumors, we describe noteworthy problems in this field that urgently need to be solved for better research and clinical application.

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“…The cisplatin-treated cancers are attenuated with the suppression of tumorigenesis and metastases [ 2 ]. However, the biological applications of cisplatin are limited to eliminating the cancers because the average morbidity and mortality statistics are high [ 3 ], which may be due to prolonged usage that influence tumour adaptability [ 4 ] drug efflux [ 5 ] or acclimatisation in various biochemical [ 6 ] and immune networks [ 7 ] that modulate cancer -induced pathophysiology with widespread toxicity in the organs [ 8 ].…”

Section: Introductionmentioning

confidence: 99%