miR-148a increases the sensitivity to cisplatin by targeting Rab14 in renal cancer cells

Kim, Eunae; Kim, Tae Ghab; Sung, Eon‐Gi; Song, In‐Hwan; Kim, Joo-Young; Doh, Kyung-Oh; Lee, Tae-Jin

doi:10.3892/ijo.2017.3851

Cited by 18 publications

(12 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As shown in the western blots, RAB14 was a target of miR-338-3p in lung adenocarcinoma cells, and hsa_ circ_0000326 overexpression contributed to elevated levels of RAB14. Alterations in RAB14 expression have been documented in gastric cancer, renal cancer, nasopharyngeal carcinoma and lung cancer [47,51,52]. As an oncogene, RAB14 participates in many biological processes, such as cell proliferation, migration and invasion, by regulating the AKT signaling pathway [51,53].…”

Section: Discussionmentioning

confidence: 99%

Circular RNA hsa_circ_0000326 acts as a miR-338-3p sponge to facilitate lung adenocarcinoma progression

Huang

et al. 2020

J Exp Clin Cancer Res

View full text Add to dashboard Cite

Background: Circular RNAs (circRNAs) are a novel class of noncoding RNAs that regulate gene expression at the transcriptional or posttranscriptional level. According to recent studies, circRNAs are involved in the pathogenesis of cancer, but the roles of circRNAs in lung adenocarcinoma are largely unknown. Methods: In this study, we identified a novel upregulated circRNA, hsa_circ_0000326, in human lung adenocarcinoma tissues using microarray analysis and qRT-PCR. We then explored the biological role of hsa_circ_0000326 using gainand loss-of-function assays in adenocarcinoma cells. Bioinformatics databases were used to screen for potential target miRNAs and the luciferase reporter assays and RNA-FISH further validated the interaction. Downstream protein was detected by western blot. Finally, we established xenografts in nude mice to assess the function of hsa_circ_0000326 in vivo. Results: We found that high expression of hsa_circ_0000326 was correlated with tumor size, regional lymph node status and differentiation in human lung adenocarcinoma. Additionally, we conducted gain-and loss-of-function assays and found that hsa_circ_0000326 acted as a positive regulator of cell proliferation and migration and a negative regulator of apoptosis. Mechanistic studies showed that hsa_circ_0000326 acted as a miR-338-3p sponge and altered the function of miR-338-3p, which in turn upregulated the expression of the downstream target RAB14 and affected the proliferation, migration and apoptosis of lung adenocarcinoma cells. Conclusions: Collectively, our study results reveal crucial roles for hsa_circ_0000326 in the proliferation, migration and apoptosis of lung adenocarcinoma cells and suggest that hsa_circ_0000326 may represent a potential therapeutic target in patients with lung adenocarcinoma.

show abstract

Section: Discussionmentioning

confidence: 99%

Circular RNA hsa_circ_0000326 acts as a miR-338-3p sponge to facilitate lung adenocarcinoma progression

Huang

et al. 2020

J Exp Clin Cancer Res

View full text Add to dashboard Cite

show abstract

“…Evidence for the influence of both of these miRNAs has been published widely in other tumor types. miR-130a influences response to chemotherapy in various tumor types such as ovarian cancer 11 , breast cancer 42 and hepatocellular carcinoma 21 , and miR-148a was attributed a central role in drug response in renal 24 and prostate cancer 43 . However, the literature reports partly contradictory results in terms of basal miR-130a expression and intrinsic chemoresistance of various cancer cell lines.…”

Section: Discussionmentioning

confidence: 99%

Complex role of miR-130a-3p and miR-148a-3p balance on drug resistance and tumor biology in esophageal squamous cell carcinoma

Eichelmann

Matuszcak

Lindner

et al. 2018

Sci Rep

View full text Add to dashboard Cite

miRNAs play a crucial role in cancer development and progression. However, results on the impact of miRNAs on drug sensitivity and tumor biology vary, and most studies to date focussed on either increasing or decreasing miRNA expression levels. Therefore, the current study investigated the role of different expression levels of miR-130a-3p and miR-148a-3p on drug resistance and tumor biology in four esophageal squamous cell carcinoma cell lines. Interestingly, up- and downregulation of both miRNAs significantly increased sensitivity towards chemotherapy. MiRNA modulation also reduced adherence and migration potential, and increased apoptosis rates. Target analyses showed that up- and downregulation of both miRNAs activated the apoptotic p53-pathway via increased expression of either BAX (miR-148a-3p) or Caspase 9 (miR-130a-3p). miR-148a-3p downregulation seemed to mediate its effects primarily via regulation of Bim rather than Bcl-2 levels, whereas we found the opposite scenario following miR-148a-3p upregulation. A similar effect was observed for miR-130a-3p regulating Bcl-2 and XIAP. Our data provide the first evidence that miRNA modulation in both directions may lead to similar effects on chemotherapy response and tumor biology in esophageal squamous cell carcinoma. Most interestingly, up- and downregulation seem to mediate their effects via modulating the balance of several validated or predicted targets.

show abstract

“…A recent systematic review of observational studies investigated the prognostic significance of miR-148a in various cancers and reported a correlation between low miR-148a levels and poor overall survival in patients with cancer (6). miR-148a has been found to be dysregulated in several cancers, including ovarian cancer (7), breast cancer (8,9), colorectal cancer (10), gastrointestinal cancer (11), prostate cancer (12), hepatocellular carcinoma (13), papillary thyroid carcinoma (14) and renal cancer (15). In ovarian cancer, miR-148a is decreased in the plasma of patients compared with healthy controls and its expression level is positively associated with survival time (16).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of miR‑148a inhibits viability and invasion of ovarian cancer OVCAR3 cells by targeting FOXO3

et al. 2019

View full text Add to dashboard Cite

Decreased expression of microRNA (miR)-148a is associated with poor prognosis in ovarian cancer. The aim of the present study was to investigate the impact of miR-148a on tumor cell viability and invasion via targeting forkhead box protein O3 (FOXO3). Expression of miR-148a was detected in paired tumor and adjacent normal tissues. OVCAR3 cells were transfected with miR-148a mimic and inhibitor. Cell viability, apoptosis and invasion were determined. A luciferase reporter assay was used to study the association between miR-148a and FOXO3. In addition, the influence of miR-148a on tumor cell growth was investigated by performing xenograft assays in nude mice. RT-qPCR showed that miR-148a was downregulated in ovarian cancer tissues. Overexpression of miR-148a in OVCAR3 cells inhibited cell viability, suppressed invasion and promoted cellular apoptosis. The dual-luciferase assay indicated that miR-148a directly regulated the expression of FOXO3, a transcription factor of caspase-3. Western blotting confirmed that the expression of caspase-3 was regulated by the modulation of miR-148a expression. In vivo assays revealed that miR-148a overexpression inhibited the growth of OVCAR3 ×enograft tumors in nude mice. miR-148a is a tumor suppressor in ovarian cancer OVCAR3 cells and in nude mice. The suppressive effect is due to inhibiting cell viability and invasion as well as promoting apoptosis. These results may provide theoretical basis for targeting miR-148a in the treatment of ovarian cancer.

show abstract

miR-148a increases the sensitivity to cisplatin by targeting Rab14 in renal cancer cells

Cited by 18 publications

References 30 publications

Circular RNA hsa_circ_0000326 acts as a miR-338-3p sponge to facilitate lung adenocarcinoma progression

Circular RNA hsa_circ_0000326 acts as a miR-338-3p sponge to facilitate lung adenocarcinoma progression

Complex role of miR-130a-3p and miR-148a-3p balance on drug resistance and tumor biology in esophageal squamous cell carcinoma

Overexpression of miR‑148a inhibits viability and invasion of ovarian cancer OVCAR3 cells by targeting FOXO3

Contact Info

Product

Resources

About