miR-145 modulates lncRNA-ROR and Sox2 expression to maintain human amniotic epithelial stem cell pluripotency and β islet-like cell differentiation efficiency

Zou, Gang; Liu, Te; Guo, Lihe; Huang, Yongyi; Feng, Ya; Huang, Qin; Duan, Tao

doi:10.1016/j.gene.2016.06.047

Cited by 37 publications

(27 citation statements)

References 37 publications

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“…Accumulating documents have demonstrated that the dysregulation of lncRNAs are associated with tumorigenesis and progression of malignant tumors (18)(19)(20)(21)(22)(23). For instance, Zhou et al demonstrated that downregulation of lncRNA MEG3 mediated by DNMT3b contributed to nickel malignant transformation of human bronchial epithelial cells via modulating PHLPP1 transcription and HIF-1alpha translation (11).…”

Section: Discussionmentioning

confidence: 99%

lncRNA SNHG5 is associated with poor prognosis of bladder cancer and promotes bladder cancer cell proliferation through targeting p27

Xue

et al. 2017

Oncol Lett

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Abstract. Long non-coding RNAs (lncRNAs) have been identified as critical regulators in tumorigenesis. In our present study, we measured the level of small nucleolar RNA host gene 5 (SNHG5) in bladder cancer (BC) tissues and cell lines, and the correlation of the level of SNHG5 with clinicopathological features and prognosis of BC patients was analyzed. Reverse transcription-quantitative polymerase chain reaction was performed to determine the level of SNHG5 in the BC tissues and cell lines. The Kaplan-Meier method was used to analyze the long-term survival outcomes. MTT and colony formation assays were applied to assess the influence of SNHG5 on cell proliferation ability. Flow cytometry was used to measure the function of SNHG5 on cell cycle and apoptosis rate. SNGH5 was found upregulated in BC tissues and cell lines and a high level of SNGH5 was correlated with a poor prognosis. Silencing SNHG5 inhibited the proliferation ability of BC cells and such a function was attributed to its influence on cells cycle and apoptosis. Our findings imply that SNHG5 was upregulated in BC tissues and played an important role in BC progression and may be a potential therapeutic target for BC patients.

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Section: Discussionmentioning

confidence: 99%

lncRNA SNHG5 is associated with poor prognosis of bladder cancer and promotes bladder cancer cell proliferation through targeting p27

Xue

et al. 2017

Oncol Lett

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“…Mechanisms of lncRNA regulating gene expression are mainly at three different levels: the transcriptional level, the post-transcriptional level and the epigenetic regulation level [35]. It has been reported that lncROR could function as competing endogenous RNA, therefore release the miRNA-combined target genes [36-38]. Moreover, lncROR has also been documented as a necessary decoy oncoRNA that plays an important regulatory role in tumorigenesis and represents a novel style of histone modification [39].…”

Section: Discussionmentioning

confidence: 99%

LncROR Promotes Bladder Cancer Cell Proliferation, Migration, and Epithelial-Mesenchymal Transition

Chen

Peng

et al. 2017

Cell Physiol Biochem

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Background: LncRNA ROR, a tumor oncogene associated with various human cancers, has been reported to be involved in regulating various cellular processes, such as proliferation, apoptosis and invasion through targeting multiple genes. However, the molecular biological function in bladder cancer has not been clearly elucidated. The aim of this study is to explore ROR expression levels and evaluated its function in bladder cancer. Methods: LncRNA ROR expression levels in the 36 pairs of bladder cancer tissues (and corresponding non-tumor tissues) and bladder cancer cells were assessed by qRT-PCR. MTT assay, colony formation assay, flow cytometric analysis, wound healing assay, cell transwell assays, attachment/detachment and western blotting were performed to assess the effects of ROR on proliferation, apoptosis, migration/invasion and epithelial-to-mesenchymal (EMT) phenotypes in BC cells in vitro. ZEB1 is target of ROR. Rescue assays were performed to further confirm that ROR contributes to the progression of BC cells through targeting ZEB1. Results: LncRNA ROR was up-regulated in bladder cancer tissues (compared to adjacent non-tumor tissues) and was almost overexpression in bladder cancer cells (compared with normal urothelial cell line SVHUC-1 cells). Increased lncRNA ROR expression significantly promoted tumor cells proliferation, inhibited cells apoptosis, facilitated cells metastasis and contributed to the formation of EMT phenotype. While down-regulated ROR could obviously inhibit cells proliferation, promote cells apoptosis, inhibit metastasis and reverse EMT to MET. ZEB1 was a target gene of ROR and was positive correlation with the level of ROR in cancer tissues. Conclusion: These results indicated that lncRNA ROR was associated with tumor progression in bladder cancer cells.

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“…The separation and culture of HuAESCs were performed as described in our previous publications [1,3,4]. Briefly, human amniotic membrane was collected from the First Maternal and Child Health Centre of Shanghai, China.…”

Section: Separation and Culture Of Huaescsmentioning

confidence: 99%

“…HuAESCs are derived from foetal amniotic trophoblast cells [1][2][3][4]. In addition to epithelial cell characteristics, these cells also have stem cell characteristics [2,4]. HuAESCs can secrete a variety of cellular growth factors, such as LIF, EGF, bFGF, TGF-a/b and BMP-4, and can express a variety of stem cell markers such as Nanog, Oct4, Sox2 and Nestin [2].…”

Section: Introductionmentioning

confidence: 99%

SPION‐mediated miR‐141 promotes the differentiation of HuAESCs into dopaminergic neuron‐like cells via suppressing lncRNA‐HOTAIR

Liu

Zhang

Zheng

et al. 2018

J Cellular Molecular Medi

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In this study, a bioinformatics analysis and luciferase reporter assay revealed that microRNA‐141 could silence the expression of lncRNA‐HOTAIR by binding to specific sites on lncRNA‐HOTAIR. We used superparamagnetic iron oxide nanoparticles (SPIONs) to mediate the high expression of microRNA‐141 (SPIONs@miR‐141) in human amniotic epithelial stem cells (HuAESCs), which was followed by the induction of the differentiation of HuAESCs into dopaminergic neuron‐like cells (iDNLCs). qPCR, western blot, immunofluorescence staining and HPLC all suggested that SPION‐mediated overexpression of miR‐141 could promote an increased expression of brain‐derived neurotrophic factor (BDNF), DAT and 5‐TH in HuAESC‐derived iDNLCs. The RIP and ChIP assay also showed that overexpression of miR‐141 could significantly inhibit the recruitment and binding of lncRNA‐HOTAIR to EZH2 on BDNF gene promoter. cDNA microarray analysis revealed that the expression levels of 190 genes were much higher in iDNLCs than in HuAESCs. Finally, a protein interaction network analysis and identification showed that in the iDNLC group with SPIONs@miR‐141, factors that interact with BDNF, such as FGF8, SHH, NTRK3 and CREB1, all showed significantly higher expression levels compared with those in the SPIONs@miR‐Mut. Therefore, this study confirmed that the highly efficient expression of microRNA‐141 mediated by SPIONs could improve the efficiency of HuAESCs differentiation into dopaminergic neuron‐like cells.

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miR-145 modulates lncRNA-ROR and Sox2 expression to maintain human amniotic epithelial stem cell pluripotency and β islet-like cell differentiation efficiency

Cited by 37 publications

References 37 publications

lncRNA SNHG5 is associated with poor prognosis of bladder cancer and promotes bladder cancer cell proliferation through targeting p27

lncRNA SNHG5 is associated with poor prognosis of bladder cancer and promotes bladder cancer cell proliferation through targeting p27

LncROR Promotes Bladder Cancer Cell Proliferation, Migration, and Epithelial-Mesenchymal Transition

SPION‐mediated miR‐141 promotes the differentiation of HuAESCs into dopaminergic neuron‐like cells via suppressing lncRNA‐HOTAIR

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