MiR-145 mediates cell morphology-regulated mesenchymal stem cell differentiation to smooth muscle cells

Yeh, Yi Ting; Wei, Josh; Thorossian, Satenick; Nguyen, Katherine T.; Hoffman, Clarissa; Álamo, Juan C. del; Serrano, Ricardo; Li, Yi Shuan Julie; Wang, Kuei Chun; Chien, Shu

doi:10.1016/j.biomaterials.2019.03.003

Cited by 34 publications

(33 citation statements)

References 44 publications

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“…Specific miRNA related to the selected genes (DNMT1 and OCT4), also involved in adipogenic and osteogenic differentiation, were identified in silico and further confirmed by literature [25,26,27,28,29,30,31,32]. In particular miR-185-3p and miR-148a-3p targeted DNMT1 (Table 1), whereas miR-145-5p was identified to target OCT4 gene (Table 1).…”

Section: Resultssupporting

confidence: 70%

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Epigenetics, Stem Cells, and Autophagy: Exploring a Path Involving miRNA

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Campesi

Cruciani

et al. 2019

IJMS

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MiRNAs, a small family of non-coding RNA, are now emerging as regulators of stem cell pluripotency, differentiation, and autophagy, thus controlling stem cell behavior. Stem cells are undifferentiated elements capable to acquire specific phenotype under different kind of stimuli, being a main tool for regenerative medicine. Within this context, we have previously shown that stem cells isolated from Wharton jelly multipotent stem cells (WJ-MSCs) exhibit gender differences in the expression of the stemness related gene OCT4 and the epigenetic modulator gene DNA-Methyltransferase (DNMT1). Here, we further analyze this gender difference, evaluating adipogenic and osteogenic differentiation potential, autophagic process, and expression of miR-145, miR-148a, and miR-185 in WJ-MSCs derived from males and females. These miRNAs were selected since they are involved in OCT4 and DNMT1 gene expression, and in stem cell differentiation. Our results indicate a difference in the regulatory circuit involving miR-148a/DNMT1/OCT4 autophagy in male WJ-MSCs as compared to female cells. Moreover, no difference was detected in the expression of the two-differentiation regulating miRNA (miR-145 and miR-185). Taken together, our results highlight a different behavior of WJ-MSCs from males and females, disclosing the chance to better understand cellular processes as autophagy and stemness, usable for future clinical applications.

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Section: Resultssupporting

confidence: 70%

“…In particular miR-185-3p and miR-148a-3p targeted DNMT1 (Table 1), whereas miR-145-5p was identified to target OCT4 gene (Table 1). [25,26,27,28,29,30,31,32,33,34,35,36].…”

Section: Resultsmentioning

confidence: 99%

Epigenetics, Stem Cells, and Autophagy: Exploring a Path Involving miRNA

Balzano

Campesi

Cruciani

et al. 2019

IJMS

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“…4 B). Since cytoskeletal organization could be controlled by Rho/ROCK signaling pathway [ 31 ], we examined the role of Rho/ROCK in ATO reinforcing the cytoskeleton and detected cell size and cell stiffness of VSMCs by treating cells with Y27632 (a ROCK inhibitor) for 4 h. In comparison with the untreated control group, inhibition of RhoA/Rho-associated kinase (ROCK) pathway significantly increased in actin assembly rates ( Fig. 4 C and D) and cell area ( Fig.…”

Section: Resultsmentioning

confidence: 99%

A novel mechanism of inhibiting in-stent restenosis with arsenic trioxide drug-eluting stent: Enhancing contractile phenotype of vascular smooth muscle cells via YAP pathway

Zhao

Zang

Yin

et al. 2021

Bioactive Materials

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Objective Arsenic trioxide (ATO or As 2 O 3 ) has beneficial effects on suppressing neointimal hyperplasia and restenosis, but the mechanism is still unclear. The goal of this study is to further understand the mechanism of ATO's inhibitory effect on vascular smooth muscle cells (VSMCs). Methods and results Through in vitro cell culture and in vivo stent implanting into the carotid arteries of rabbit, a synthetic-to-contractile phenotypic transition was induced and the proliferation of VSMCs was inhibited by ATO. F-actin filaments were clustered and the elasticity modulus was increased within the phenotypic modulation of VSMCs induced by ATO in vitro . Meanwhile, Yes-associated protein (YAP) nuclear translocation was inhibited by ATO both in vivo and in vitro . It was found that ROCK inhibitor or YAP inactivator could partially mask the phenotype modulation of ATO on VSMCs. Conclusions The interaction of YAP with the ROCK pathway through ATO seems to mediate the contractile phenotype of VSMCs. This provides an indication of the clinical therapeutic mechanism for the beneficial bioactive effect of ATO-drug eluting stent (AES) on in-stent restenosis (ISR).

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“…This attention. [40][41][42] The cells at the edge of colonies of undifferentiated human pluripotent stem cells show distinct actin organization and greater myosin activity compared with those in the interior. 43 Our recent work also indicated that F-actin bundles located in cells at the outer edge of the colony form a reticulate structure, which plays a mechanical role in the growth of the mESC colony.…”

Section: Discussionmentioning

confidence: 99%

A function of fascin1 in the colony formation of mouse embryonic stem cells

Fan

Zhao

et al. 2020

Stem Cells

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Fascin1 is known to participate in the migration of cancer cells by binding to actin filaments. Recent studies evidenced that fascin1 also modulates processes such as the tumorigenesis and maintenance of pluripotency genes in cancer stem cells. However, the function of fascin1 in embryonic stem cells remains unclear. In this article, we report that fascin1 is highly expressed and widely distributed in mouse embryonic stem cells (mESCs), which are regulated by JAK‐STAT3 and β‐catenin. We found that the overexpression of fascin1 impairs the formation of mESC colonies via the downregulation of intercellular adhesion molecules, and that mimicking the dephosphorylated mutation of fascin1 or inhibiting phosphorylation with Gö6983 significantly enhances colony formation. Hyperphosphorylated fascin1 can promote the maintenance of pluripotency in mESCs via nuclear localization and suppressing DNA methyltransferase expression. Our findings demonstrate a novel function of fascin1, as a vital regulator, in the colony formation and pluripotency of mESCs and provide insights into the molecular mechanisms underlying embryonic stem cell self‐organization and development in vitro.

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MiR-145 mediates cell morphology-regulated mesenchymal stem cell differentiation to smooth muscle cells

Cited by 34 publications

References 44 publications

Epigenetics, Stem Cells, and Autophagy: Exploring a Path Involving miRNA

Epigenetics, Stem Cells, and Autophagy: Exploring a Path Involving miRNA

A novel mechanism of inhibiting in-stent restenosis with arsenic trioxide drug-eluting stent: Enhancing contractile phenotype of vascular smooth muscle cells via YAP pathway

A function of fascin1 in the colony formation of mouse embryonic stem cells

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