miR-142 keeps CD4+ DCs in balance

Belz, Gabrielle T.

doi:10.1182/blood-2012-12-472589

Cited by 10 publications

(16 citation statements)

References 10 publications

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“…Of note, Th17 cells can differentiate into Treg cells during the resolution of inflammation (Gagliani et al ., ), as well as into the Th2 subset in response to IL‐4 (Lee et al ., ), and MNC treatment promoted a higher degree of plasticity among these T cell subsets, particularly between Th17 and Th2. Additionally, CCL2 enhances IL‐4 secretion by T cells and elicits Th2 polarising effects, and miR‐142 has an important role in DC priming of Th2 responses (Gu et al ., ; Belz, ), both up‐regulated in MNC‐treated mice.…”

Section: Discussionmentioning

confidence: 95%

Immunomodulatory tetracyclines shape the intestinal inflammatory response inducing mucosal healing and resolution

Garrido-Mesa

Rodríguez-Nogales

Algieri

et al. 2018

British J Pharmacology

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Section: Discussionmentioning

confidence: 95%

Immunomodulatory tetracyclines shape the intestinal inflammatory response inducing mucosal healing and resolution

Garrido-Mesa

Rodríguez-Nogales

Algieri

et al. 2018

British J Pharmacology

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“…Hematopoietic-specific miR-142 has emerged in recent years as a critical regulator of various blood and lymphoid cell lineages (Gauwerky et al, 1989; Chen et al, 2004; Huang et al, 2009; Belz, 2013; Lagrange et al, 2013; Lu et al, 2013; Mildner et al, 2013; Nimmo et al, 2013; Sonda et al, 2013; Sun et al, 2013; Zhou et al, 2013). Our analysis unveils the critical functions of miR-142 in the MK lineage.…”

Section: Discussionmentioning

confidence: 99%

“…Pioneering experimental evidence has suggested miR-142 involvement in lymphocyte differentiation (Chen et al, 2004) and recently, miR-142 was also shown to play a role in the specification of definitive hemangioblasts (Lu et al, 2013; Nimmo et al, 2013), and in lymphoid and myeloid lineages (Gauwerky et al, 1989; Chen et al, 2004; Huang et al, 2009; Belz, 2013; Lagrange et al, 2013; Lu et al, 2013; Nimmo et al, 2013; Sonda et al, 2013; Sun et al, 2013; Zhou et al, 2013). Furthermore, miR-142 is involved in the compound immune response to North American eastern equine encephalitis virus (Trobaugh et al, 2014) and our work uncovered a key role for miR-142 in the maintenance of CD4 + dendritic cells (Mildner et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, miR-142 is involved in compound immune response to North American eastern equine encephalitis virus (Trobaugh et al, 2014) and our work uncovered a key role for miR-142 in the maintenance of CD4+ dendritic cells (Mildner et al, 2013). ” And in the Discussion section: “Hematopoietic–specific miR-142 emerges in recent years as a critical regulator of various blood and Lymph cell lineages (Belz, 2013; Chen et al, 2004; Gauwerky et al, 1989; Huang et al, 2009; Lagrange et al, 2013; Lu et al, 2013; Mildner et al, 2013; Nimmo et al, 2013; Sonda et al, 2013; Sun et al, 2013; Zhou et al, 2013)…”…”

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confidence: 99%

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miR-142 orchestrates a network of actin cytoskeleton regulators during megakaryopoiesis

Chapnik

Rivkin

Mildner

et al. 2014

eLife

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Genome-encoded microRNAs (miRNAs) provide a posttranscriptional regulatory layer that controls the differentiation and function of various cellular systems, including hematopoietic cells. miR-142 is one of the most prevalently expressed miRNAs within the hematopoietic lineage. To address the in vivo functions of miR-142, we utilized a novel reporter and a loss-of-function mouse allele that we have recently generated. In this study, we show that miR-142 is broadly expressed in the adult hematopoietic system. Our data further reveal that miR-142 is critical for megakaryopoiesis. Genetic ablation of miR-142 caused impaired megakaryocyte maturation, inhibition of polyploidization, abnormal proplatelet formation, and thrombocytopenia. Finally, we characterized a network of miR-142-3p targets which collectively control actin filament homeostasis, thereby ensuring proper execution of actin-dependent proplatelet formation. Our study reveals a pivotal role for miR-142 activity in megakaryocyte maturation and function, and demonstrates a critical contribution of a single miRNA in orchestrating cytoskeletal dynamics and normal hemostasis.DOI: http://dx.doi.org/10.7554/eLife.01964.001

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“…There are many reports that indicated the role of miRs in regulation of immune response even innate or acquired immunity (Baltimore, Boldin, O'connell, Rao, & Taganov, ; Lindsay, ). Moreover, it is widely recognized that some miRNAs including miR‐155, Let‐7i, miR‐22, miR‐21, miR‐146, miR‐34a, and miR‐142 are involved in DC differentiation and maturation (Belz, ; Mildner et al, ; Smyth, Boardman, Tung, Lechler, & Lombardi, ; So, Winchester, & Ouchi, ; Sun et al, ; Zhou & Wu, ). Mechanistically, miR‐142 upregulated IRF8 which regulate CD4 + DCs differentiation (Belz, ; Mildner et al, ).…”

Section: Introductionmentioning

confidence: 99%

microRNA modified tumor‐derived exosomes as novel tools for maturation of dendritic cells

Khani

Hassan

Ebrahimi

et al. 2018

Journal Cellular Physiology

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Tumor‐derived exosomes (TEX) are known by their immune suppression effects as well as initiation mediators in cancer progression and metastasis. Meanwhile, they are appropriate sources to induce immunity against tumor cells, as consist of tumor specific and associated antigens. The aim of the current study is modifying TEX with microRNA miR‐155, miR‐142, and let‐7i, to enhance their immune stimulation ability and induce potent dendritic cells (DC). For this, exosomes were isolated from mouse mammalian breast cancer cell line; 4T1, and subjected to miR‐155, miR‐142, and let‐7i by electroporation. Immature DCs were generated from mouse bone marrow in the presence of interleukin‐4 (IL‐4) and granulocyte‐macrophage colony‐stimulating factor (GM‐CSF). To mature DCs, lipopolysaccharide (LPS), TEX, and modified TEX were used. The expression level of miRNAs and their target genes (IL‐6, IL‐17, IL‐1b, TGFβ, SOCS1, KLRK1, IFNγ, and TLR4) was determined. TEX were nanovesicles with spheroid morphology which expressed CD81, CD63, and TSG101, as exosome markers, at protein level. MHCII, CD80, and CD40 as maturation markers were assessed by flow cytometry. Overexpression of miRNAs were confirmed in exosomes and mDCs. Up and downregulation of target genes confirmed the gene network in DC maturation. We found that Let‐7i could efficiently induce the DC maturation, as well as miR‐142 and miR‐155 have enhancing effects. These findings reveal that the modified TEX would be a hopeful cell‐free vaccine for the cancer treatment.

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miR-142 keeps CD4+ DCs in balance

Cited by 10 publications

References 10 publications

Immunomodulatory tetracyclines shape the intestinal inflammatory response inducing mucosal healing and resolution

Immunomodulatory tetracyclines shape the intestinal inflammatory response inducing mucosal healing and resolution

miR-142 orchestrates a network of actin cytoskeleton regulators during megakaryopoiesis

microRNA modified tumor‐derived exosomes as novel tools for maturation of dendritic cells

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