miR-140-5p inhibits the proliferation and enhances the efficacy of doxorubicin to breast cancer stem cells by targeting Wnt1

Wu, Dawei; Zhang, Jun; Lü, Ying; Song, Bo; Li, Lianhong; Wang, Lu; Zhang, Qingqing; Murata, Jun

doi:10.1038/s41417-018-0035-0

Cited by 59 publications

(34 citation statements)

References 31 publications

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“…The expression of miR-93 was upregulated, and cell proliferation was promoted in the insulin-stimulated KGN granulosa cells [10]. In recent years, miR-140 has been reported to exhibit significant regulatory effects with respect to cell proliferation, differentiation, migration, and invasion [11][12][13]. Scalici et al showed that the expression of miR-140 in the follicular fluid was different between PCOS and normal ovarian reserves, with a specificity of 83.8% and a sensitivity of 70% [14].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: MiR-140 targets RAP2A to enable the proliferation of insulin-treated ovarian granulosa cells

Xiong

Wang

et al. 2020

J Ovarian Res

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Background: We elucidated the role of specific MicroRNAs (miRNAs) in the development of polycystic ovary syndrome (PCOS) and explained the changes in the proliferation of granulosa cells. Excised ovarian cortex specimens were collected for miRNA profiling analysis (n = 20 PCOS females and 5 non-PCOS females). Insulintreated ovarian granulosa cells isolated from mice were used for mechanical studies. Results: High miR-140 expression was observed in PCOS samples and insulin-treated granulosa cells compared to that in non-PCOS and unstimulated cells, respectively. However, the Ras-related protein Rap-2a precursor (RAP2A) was downregulated in in PCOS. MTT assay and EdU staining showed that an miR-140 inhibitor attenuated viability in insulin-treated granulosa cells; cell viability increased with miR-140 overexpression. Reduced expression of miR-140 and the expression of the miR-140 mimic resulted in marked cell apoptosis, as evidenced by the results of PI flow cytometry and Annexin V-FITC; miR-140 overexpression results in downregulated RAP2A expression, and the miR-140 mimic directly bound to the RAP2A 3′-UTR, causing increase in RAP2A levels in insulin-treated granulosa cells; RNA-mediated silencing of RAP2A in insulin-treated granulosa cells restored cell proliferation and apoptosis to normal levels. Phosphorylated AKT was found to be negatively regulated through cross-talk between miR-140 and RAP2A. Conclusions: In conclusion, PCOS ovarian cortex specimens and insulin-treated granulosa cells showed elevated expression of miR-140, which could lead to increased proliferation and reduced apoptosis of cells by targeting RAP2A. This study may pave the way for future research on the properties of granulosa cells in PCOS.

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: MiR-140 targets RAP2A to enable the proliferation of insulin-treated ovarian granulosa cells

Xiong

Wang

et al. 2020

J Ovarian Res

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“…25,26 The expression of miR-140 is decreased in breast cancer stem cells (BCSCs), and miR-140 acts as a tumour suppressor gene to inhibit tumour proliferation by regulating Wnt1. 27 It has been reported that miR-185 expression is downregulated in some tumours and that upregulation of miR-185 expression significantly inhibits the growth, migration, and invasion of Ewing's sarcoma cells. Inconsistent with miRNA microarray results, the expression levels of miR-4505 and miR-4741 were higher in myeloma exosomes than HCs.…”

Section: Discussionmentioning

confidence: 99%

Serum exosomal microRNAs as novel biomarkers for multiple myeloma

Zhang

Geng

et al. 2019

Hematological Oncology

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Accumulating studies have focused on circulating microRNAs, which might be potential biomarkers for different malignancies. The aim of this study was to investigate the potential of serum exosomal microRNAs to be novel serum biomarkers for smouldering myeloma (SMM) or even multiple myeloma (MM). The levels of serum exosomal microRNAs and serum circulating microRNAs were measured in healthy individuals and patients with SMM (n = 20) or MM (n = 20). Serum exosomal microRNAs and serum circulating microRNAs were extracted from serum, and the expression levels of selected microRNAs were quantified by real‐time polymerase chain reaction (PCR). The levels of serum exosome‐derived miR‐20a‐5p, miR‐103a‐3p, and miR‐4505 were significantly different among patients with MM, patients with SMM, and healthy individuals, while there were differences in the levels of let‐7c‐5p, miR‐185‐5p, and miR‐4741 in patients with MM relative to those in SMM patients or healthy controls. Additionally, a significant correlation was rarely found between the levels of serum and exosomal microRNAs. This study shows that serum exosomal microRNAs can be used independently as novel serum biomarkers for MM.

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“…Mimics of miRNA-140-5p enhanced sensitivity of doxorubicin treatment via the Wnt1/ABCB1 pathway both in vitro and in vivo. Therefore, these findings would suggest increased miRNA-140-5p expression levels may benefit patient response to doxorubicin treatment and reduce chemoresistance [150].…”

Section: Induced Mirna-140-5p Levels In Breast Cancer May Enhance Outmentioning

confidence: 95%

“…Expression of miRNA-140-5p is frequently down regulated in breast cancer stem cells and its inhibitory effects on proliferation have been linked to Wnt1 [150]. Mimics of miRNA-140-5p enhanced sensitivity of doxorubicin treatment via the Wnt1/ABCB1 pathway both in vitro and in vivo.…”

Section: Induced Mirna-140-5p Levels In Breast Cancer May Enhance Outmentioning

confidence: 99%

MiRNAs as Novel Adipokines: Obesity-Related Circulating MiRNAs Influence Chemosensitivity in Cancer Patients

Withers

Dewhurst

Hammond

et al. 2020

ncRNA

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Adipose tissue is an endocrine organ, capable of regulating distant physiological processes in other tissues via the release of adipokines into the bloodstream. Recently, circulating adipose-derived microRNAs (miRNAs) have been proposed as a novel class of adipokine, due to their capacity to regulate gene expression in tissues other than fat. Circulating levels of adipokines are known to be altered in obese individuals compared with typical weight individuals and are linked to poorer health outcomes. For example, obese individuals are known to be more prone to the development of some cancers, and less likely to achieve event-free survival following chemotherapy. The purpose of this review was twofold; first to identify circulating miRNAs which are reproducibly altered in obesity, and secondly to identify mechanisms by which these obesity-linked miRNAs might influence the sensitivity of tumors to treatment. We identified 8 candidate circulating miRNAs with altered levels in obese individuals (6 increased, 2 decreased). A second literature review was then performed to investigate if these candidates might have a role in mediating resistance to cancer treatment. All of the circulating miRNAs identified were capable of mediating responses to cancer treatment at the cellular level, and so this review provides novel insights which can be used by future studies which aim to improve obese patient outcomes.

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miR-140-5p inhibits the proliferation and enhances the efficacy of doxorubicin to breast cancer stem cells by targeting Wnt1

Cited by 59 publications

References 31 publications

RETRACTED ARTICLE: MiR-140 targets RAP2A to enable the proliferation of insulin-treated ovarian granulosa cells

RETRACTED ARTICLE: MiR-140 targets RAP2A to enable the proliferation of insulin-treated ovarian granulosa cells

Serum exosomal microRNAs as novel biomarkers for multiple myeloma

MiRNAs as Novel Adipokines: Obesity-Related Circulating MiRNAs Influence Chemosensitivity in Cancer Patients

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