miR-1388 regulates the expression of nectin2l in Paralichthys olivaceus

Yang, Fan; Guan, Jian; Li, Rui; Li, Xuemei; Niu, Jingjing; Shang, Renjie; Qi, Jie; Wang, Xubo

doi:10.1016/j.cbd.2018.05.003

Cited by 9 publications

(8 citation statements)

References 45 publications

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“…On the other hand, miR‐200a has been reported in endometrial tissue of patients with endometriosis associated with the occurrence of infertility, and the high expression of miR‐200a in the endometrium can regulate the expression of the downstream target genes to influence the endometrial receptivity and thus cause the occurrence of infertility (Shu, 2017). In a previous study, miR‐200a was found to be significantly upregulated in testis, sperm and seminal plasma of infertile males compared with normozoospermic men (Yang, Guan, et al., 2018; Mokánszki et al., 2019). miR‐200a which has been demonstrated in embryos of zebrafish at early stages of development triggered cell cycle arrest and induced apoptosis, thereby inhibiting somatic growth (Riesco, Valcarce, Martínez‐Vázquez, & Robles, 2019).…”

Section: Discussionmentioning

confidence: 89%

Comparative rna‐seq analysis of region‐specific miRNA expression in the epididymis of cattleyak

Zhao

Solangi

et al. 2021

Reprod Domestic Animals

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The epididymis is the site of post‐testicular sperm maturation, which constitutes the acquisition of sperm motility and the ability to recognize and fertilize oocytes. The role of miRNA in male reproductive system, including the control of different steps leading to proper fertilization such as gametogenesis, sperm maturation and maintenance of male fertility where the deletion of Dicer in mouse germ cells led to infertility, has been demonstrated. The identification of miRNA expression in a region‐specific manner will therefore provide valuable insight into the functional differences between the regions of the epididymis. In this study, we employed RNA‐seq technology to explore the expression pattern of miRNAs and establish some miRNAs of significant interest with regard to epididymal sperm maturation in the CY epididymis. We identified a total of 431 DE known miRNAs; 119, 185 and 127 DE miRNAs were detected for caput versus corpus, corpus versus cauda and caput versus cauda region pairs, respectively. Our results demonstrate region‐specific miRNA expression in the CY epididymis. The GO and KEGG enrichment for the predicted target genes indicated the functional values of miRNAs. Furthermore, we observed that the expression of miR‐200a was downregulated in the caput, compared with cauda. Since the family of miR‐200 has previously been suggested to contribute to the distinct physiological function of sperm maturation in epididymis of adult rat, we speculate that the downregulation of miR‐200a in CY caput epididymis may play an important role of sperm maturation in the epididymis of CY. Therefore, our findings may not only increase our understanding of the molecular mechanisms regulated by the miRNA functions in region‐specific miRNA expression in the CY epididymis, it could provide a valuable information to understand the mechanism of male infertility of CY.

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Section: Discussionmentioning

confidence: 89%

Comparative rna‐seq analysis of region‐specific miRNA expression in the epididymis of cattleyak

Zhao

Solangi

et al. 2021

Reprod Domestic Animals

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“…The 38 overlapped DE lncRNAs had unknown functions. As for the overlapped DE miRNAs, miR-26a (Kang et al, 2017) and miR-1388 (Yang et al, 2018) have been proven to be correlated with gonadal development in sh.…”

Section: Resultsmentioning

confidence: 99%

“…miR-26a-5p overexpression can inhibit TNRC6A expression and promote the proliferation of the theca cells (Kang et al, 2017). miR-1388 plays an indispensable role in regulating gonadal development in P. olivaceus through its target, the nectin21 gene (Yang et al, 2018). The role of these lncRNAs, mRNAs, and miRNAs from this predicted ceRNA network in gonadal development and meiosis may provide a resource for mechanism research.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of miRNA-mRNA/lncRNA during gonadal development of triploid female rainbow trout (Oncorhynchus mykiss)

et al. 2021

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Background: Chromosomal ploidy manipulation is one of the means to create excellent germplasm. Triploid sh could provide an ideal sterile model for the mechanism research of abnormality in meiosis. The complete understanding of the coding and noncoding RNAs regulating sterility caused by meiosis abnormality is still not well understood.Results: By high-throughput sequencing, we compared the expression pro les of gonadal mRNA, long non-coding RNA (lncRNA), and microRNA (miRNA) at different developmental stages [65 days post fertilisation (dpf), 180 dpf, and 600 dpf] between the diploid (XX) and triploid (XXX) female rainbow trout. A majority of differentially expressed (DE) RNAs were identi ed, and 22 DE mRNAs related to oocyte meiosis and homologous recombination were characterized. The predicted miRNA-mRNA/lncRNA networks of 3 developmental stages were constructed based on the target pairs of DE lncRNA-miRNA and DE mRNA-miRNA. According to the networks, meiosis-related gene of ccne1 was targeted by dre-miR-15a-5p_R+1, and 6 targeted DE lncRNAs were identi ed. Also, RT-qPCR was performed to validate the credibility of the network.Conclusions: This study explored the potential interplay between coding and noncoding RNAs during the gonadal development of polyploid sh. It provides full insights into polyploidy-associated effects on fertility of sh. These differentially expressed coding and noncoding RNAs provide a novel resource for studying genome diversity of polyploid induction.

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“…miR-26a-5p overexpression can inhibit TNRC6A expression and promote the proliferation of the theca cells (Kang et al, 2017). miR-1388 plays an indispensable role in regulating gonadal development in P. olivaceus through its target, the nectin21 gene (Yang et al, 2018). The role of these lncRNAs, mRNAs, and miRNAs from this predicted ceRNA network in gonadal development and meiosis of reproductive cells requires further in-depth research.…”

Section: Discussionmentioning

confidence: 99%

Section: Functional Analysis Of De Mrnas De Lncrnas and De Mirnas Dmentioning

confidence: 99%

Comprehensive Analysis of miRNA-mRNA/lncRNA During Gonadal Development of Triploid Rainbow Trout (Oncorhynchus mykiss)

Huang

Liu

Shi

et al. 2020

Preprint

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Background: Polyploidy is an important force to improve biological evolution. Chromosomal ploidy manipulation is one of the means to create excellent germplasm. It has an important significance to understand the effect of genome duplication on fertility in polyploidy breeding. In this study, coding and non-coding RNAs involved in gonadal development were characterized, and their relationships were explored. Results: By high-throughput sequencing, we compared the expression profiles of gonadal mRNA, long non-coding RNA (lncRNA), and microRNA (miRNA) at different developmental stages [65 days post fertilisation (dpf), 180 dpf, and 600 dpf] between the diploid (XX) and triploid (XXX) rainbow trout. A majority of differentially expressed (DE) RNAs was screened. The overlapped DE mRNAs of three stages were functionally annotated by Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis. According to the overlapped miRNAs, the predicted miRNA-mRNA/lncRNA network was constructed based on the target pairs of lncRNA-miRNA and mRNA-miRNA. Also, RT-qPCR was performed to validate the credibility of the network. Conclusions: Lots of lncRNAs involved in fertility were characterised by this network, which provided a reference sequence for further research. In summary, this study explored the potential interplay between coding and noncoding RNAs during the gonadal development of polyploid fish and elaborated the effect of triploidization on fertility.

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miR-1388 regulates the expression of nectin2l in Paralichthys olivaceus

Cited by 9 publications

References 45 publications

Comparative rna‐seq analysis of region‐specific miRNA expression in the epididymis of cattleyak

Comparative rna‐seq analysis of region‐specific miRNA expression in the epididymis of cattleyak

Comprehensive analysis of miRNA-mRNA/lncRNA during gonadal development of triploid female rainbow trout (Oncorhynchus mykiss)

Comprehensive Analysis of miRNA-mRNA/lncRNA During Gonadal Development of Triploid Rainbow Trout (Oncorhynchus mykiss)

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