MiR-133b Modulates the Osteoblast Differentiation to Prevent Osteoporosis Via Targeting GNB4

Wang, Jinqiang; Gao, Zhaoqing; Gao, Peng

doi:10.1007/s10528-021-10048-9

Cited by 9 publications

(13 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SPP1 is abundant in areas of endochondral ossification and intramembranous ossification and can be observed in the cytoplasm of osteoblasts and osteoblasts in woven bones. SPP1 protein contains a region rich in aspartic acid and it can combine with hydroxyapatite in tissues through this region to exert its roles (26)(27)(28). After the initiation of bone matrix mineralization, the level of SPP1 in osteoblasts begins to increase, suggesting that SPP1 exerts its functions in the termination of mineralization of osteoblasts (29).…”

Section: Discussionmentioning

confidence: 99%

Relationship between miRNA‑433 and SPP1 in the presence of fracture and traumatic brain injury

et al. 2021

View full text Add to dashboard Cite

Limb fracture combined with traumatic brain injury (TBI) is one of the most common multiple injuries and patients often suffer from severe craniocerebral injury combined with long bone fracture of the limbs. The present study examined the expression of osteopontin (SPP1) in the tibial fracture callus and heterotopic ossification tissues in craniocerebral injury and investigated its relationship with miR-433. A total of 26 patients with tibial fracture combined with brain injury were included in the TBI group, and 26 patients with simple tibial fracture were included in the control group. The patients received immobilization treatment and callus was collected during the operation. At the time of steel plate removal tissue ossification samples from patients with heterotopic ossification were collected. Peripheral blood was collected from all patients on the morning of the operation day. Expression of miR-433 and SPP1 mRNA was determined by reverse transcription-quantitative PCR and SPP1 protein expression was measured by western blotting. Dual luciferase reporter assay was used to identify the direct interaction between miR-433 and SPP1 mRNA. The human osteoblast line hFOB1.19 was transfected with agomiR-433 to overexpress miR-433 and expression of SPP1 was also examined. TBI enhanced the incidence of callus formation and heterotopic ossification in patients with fracture but did not alter fracture healing time. SPP1 mRNA and protein expression was elevated in patients who had tibial fracture in combination with craniocerebral injury in comparison with controls By contrast, expression of miR-433 was decreased in patients who had tibial fracture in combination with craniocerebral injury in comparison with controls. miR-433 regulated the expression of SPP1 mRNA and protein by directly binding to the 3'-untranslated region of SPP1 mRNA. The present study suggests that SPP1 mRNA and protein levels are increased in the callus, heterotopic ossification tissues and plasma from patients with tibial fracture combined with brain injury in comparison with controls. This elevation may be due to the reduced expression of miR-433.

show abstract

Section: Discussionmentioning

confidence: 99%

Relationship between miRNA‑433 and SPP1 in the presence of fracture and traumatic brain injury

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Li et al [ 28 ] illustrated that high expression of miR-149-3p presented a high tendency to differentiate hBMSCs into osteoblast, serving as a prospective marker for OP treatment. In our study, we stimulated hBMSCs with β-glycerophosphate and L-ascorbic acid to induce osteogenic differentiation, as described before [ 29 ]. The results demonstrated that ALP activity and Alizarin red staining were increased along with enhanced osteoblast marker expressions.…”

Section: Discussionmentioning

confidence: 99%

miR-215-5p regulates osteoporosis development and osteogenic differentiation by targeting XIAP

Yin

Shen

Wang

et al. 2022

BMC Musculoskelet Disord

View full text Add to dashboard Cite

Background Osteoporosis (OP) is a metabolic disease that involves microstructure destruction and fracture damage. The present study probed into the significance of miR-215-5p in OP progression. Methods Serum samples were collected from surgical patients and healthy controls. qRT-PCR analysis was utilized to determine the miR-215-5p level in clinical samples and human bone mesenchymal stem cells (hBMSCs) induced by β-glycerol phosphate. A dual luciferase reporter assay was exploited to examine the targeted relationship between miR-215-5p and XIAP. The mineralization and calcium deposition of hBMSCs were assessed by detection of ALP activity, Alizarin red staining, and osteoblast marker expression. Protein expression was determined by western blot analysis. Results MiR-215-5p was significantly reduced in patients with OP and increased in hBMSCs treated with β-glycerophosphate. Enhanced miR-215-5p level triggered augment in osteoblast markers (Alkaline phosphatase/ ALP, Osteocalcin/ OCN, and Runt-Related Transcription Factor 2/ Runx2), which was accompanied by the increase of ALP activity in hBMSCs and accumulation of Calcium. Functional experiments show that XIAP was a target of miR-215-5p and negatively modulated by miR-215-5p. XIAP expression levels were increased in OP samples, and decreased XIAP in β-glycerophosphate-treated hBMSCs inhibited its’ osteogenic differentiation. Functional loss and acquisition experiments depicted that miR-215-5p promoted the differentiation of hBMSCs by inhibiting the XIAP level, playing a protective role in the pathogenesis of OP. Conclusions β-glycerophosphate promoted the osteogenic differentiation of hBMSCs, increased miR-215-5p level, and decreased XIAP. miR-215-5p stimulated osteogenic differentiation of hBMSCs by targeting XIAP, shedding new insights for the detection and therapy of OP.

show abstract

“…In a separate study, MSC-EVs that originated from older rats were demonstrated to have a lower content of miR-133b-3p and miR-294, two miRNAs that inhibit TGF-b1-mediated epithelial-to-mesenchymal transition, which contributes to fibrosis (82). Reduced expression of miR-133b has also been identified in COPD patients, coronary artery disease and osteoporosis with a range of suggested functions including regulating vascular smooth muscle cells and osteoblast differentiation (83)(84)(85). These studies suggest EVs from aged MSC may be less capable of protecting against chronic inflammation and tissue damage, which in turn may promote accelerated ageing and the development of chronic diseases such as COPD.…”

Section: Evs As Immunomodulatory Factors In Ageingmentioning

confidence: 99%

The Role of Extracellular Vesicles as a Shared Disease Mechanism Contributing to Multimorbidity in Patients With COPD

et al. 2021

View full text Add to dashboard Cite

Chronic obstructive pulmonary disease (COPD) is one of the leading causes of death worldwide. Individuals with COPD typically experience a progressive, debilitating decline in lung function as well as systemic manifestations of the disease. Multimorbidity, is common in COPD patients and increases the risk of hospitalisation and mortality. Central to the genesis of multimorbidity in COPD patients is a self-perpetuating, abnormal immune and inflammatory response driven by factors including ageing, pollutant inhalation (including smoking) and infection. As many patients with COPD have multiple concurrent chronic conditions, which require an integrative management approach, there is a need to greater understand the shared disease mechanisms contributing to multimorbidity. The intercellular transfer of extracellular vesicles (EVs) has recently been proposed as an important method of local and distal cell-to-cell communication mediating both homeostatic and pathological conditions. EVs have been identified in many biological fluids and provide a stable capsule for the transfer of cargo including proteins, lipids and nucleic acids. Of these cargo, microRNAs (miRNAs), which are short 17-24 nucleotide non-coding RNA molecules, have been amongst the most extensively studied. There is evidence to support that miRNA are selectively packaged into EVs and can regulate recipient cell gene expression including major pathways involved in inflammation, apoptosis and fibrosis. Furthermore changes in EV cargo including miRNA have been reported in many chronic diseases and in response to risk factors including respiratory infections, noxious stimuli and ageing. In this review, we discuss the potential of EVs and EV-associated miRNA to modulate shared pathological processes in chronic diseases. Further delineating these may lead to the identification of novel biomarkers and therapeutic targets for patients with COPD and multimorbidities.

show abstract

MiR-133b Modulates the Osteoblast Differentiation to Prevent Osteoporosis Via Targeting GNB4

Cited by 9 publications

References 30 publications

Relationship between miRNA‑433 and SPP1 in the presence of fracture and traumatic brain injury

Relationship between miRNA‑433 and SPP1 in the presence of fracture and traumatic brain injury

miR-215-5p regulates osteoporosis development and osteogenic differentiation by targeting XIAP

The Role of Extracellular Vesicles as a Shared Disease Mechanism Contributing to Multimorbidity in Patients With COPD

Contact Info

Product

Resources

About