miR-1303 promotes the proliferation of neuroblastoma cell SH-SY5Y by targeting GSK3β and SFRP1

Li, Zuoqing; Xu, Zhe; Xie, Qiurong; Gao, Wenyuan; Xie, Julin; Zhou, Li

doi:10.1016/j.biopha.2016.07.010

Cited by 34 publications

(18 citation statements)

References 18 publications

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“…Previous studies found that miR-1303 was significantly overexpressed in GC cells and downregulation of it can inhibit proliferation, migration, and invasion of GC cells by targeting claudin-18 (CLDN18), 67 and another study also suggested that miR-1303 promotes NB proliferation by targeting glycogen synthase kinase 3 beta (GSK3β) and secreted frizzled-related protein 1 (SFRP1); 68 therefore, downregulation of miR-1303 in RTHF-treated A549 cells may play role in inhibiting the proliferation, migration, and invasion of cells by targeting some proteins, such as CLDN18, GSK3β, and SFRP1.…”

Section: Discussionmentioning

confidence: 99%

Analysis of change in microRNA expression profiles of lung cancer A549 cells treated with Radix tetrastigma hemsleyani flavonoids

Liu¹,

Xu²,

Zhang³

et al. 2018

OTT

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BackgroundThe aim of this study was to determine the inhibition effects of Radix tetrastigma hemsleyani (RTH) flavonoids on human lung adenocarcinoma A549 cells and the underlying molecular mechanism. RTH is an important Chinese traditional herb that has been widely used in cancer therapy. As an important type of active substance, RTH flavones (RTHF) have been shown to have good antiproliferative effects on various cancer cells. MicroRNAs (miRNAs) are small, noncoding RNA molecules that play important roles in cancer progression and prevention. However, the miRNA profile of RTHF-treated A549 cells has not yet been studied.Materials and methodsThe miRNA expression profile changes of A549 cell treated with RTHF were determined using the miRNA-seq analysis. Furthermore, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of differentially expressed miRNAs′ (DE-miRNAs) target genes were carried out.ResultsIn this study, we identified 162 miRNAs that displayed expression changes >1.2-fold in RTHF-treated A549 cells. GO analysis results showed that target genes of DE-miRNAs were significantly enriched in protein binding, binding, cell, cell part, intracellular, cellular process, single-organism process, and single-organism cellular process. Pathway analysis illustrated that target genes of DE-miRNAs are mainly involved in endocytosis, axon guidance, lysosome, melanogenesis, and acute myeloid leukemia pathway.ConclusionThese results may assist in the better understanding of the anticancer effects of RTHF in A549 cells.

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Section: Discussionmentioning

confidence: 99%

Analysis of change in microRNA expression profiles of lung cancer A549 cells treated with Radix tetrastigma hemsleyani flavonoids

Liu¹,

Xu²,

Zhang³

et al. 2018

OTT

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“…Several important genes were downregulated, including NEUROG2, ASCL1, SYT4, however no specific correlations with miRNAs were found. The upregulation of miR-1303 could be attributed to the nature of neuroblastoma cells, as miR-1303 has been shown to promote the proliferation of SH-SY5Y cells; several studies showed that miR-1303 is upregulated in neuroblastoma cells and tissues [20]. miR-1303 decreases doublecortin (DCX), which plays an important role in neurogenesis and its deletion causes severe morphologic defects and delayed migration along the rostral migratory stream [21].…”

Section: Discussionmentioning

confidence: 99%

Molecular Mechanisms of Bortezomib Action: Novel Evidence for the miRNA–mRNA Interaction Involvement

Łuczkowska

Rogińska

Ulańczyk

et al. 2020

IJMS

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Bortezomib is an anti-tumor agent, which inhibits 26S proteasome degrading ubiquitinated proteins. While apoptotic transcription-associated activation in response to bortezomib has been suggested, mechanisms related to its influence on post-transcriptional gene silencing mediated regulation by non-coding RNAs remain not fully elucidated. In the present study, we examined changes in global gene and miRNA expression and analyzed the identified miRNA–mRNA interactions after bortezomib exposure in human neuroblastoma cells to define pathways affected by this agent in this type of cells. Cell viability assays were performed to assess cytotoxicity of bortezomib. Global gene and miRNA expression profiles of neuroblastoma cells after 24-h incubation with bortezomib were determined using genome-wide RNA and miRNA microarray technology. Obtained results were then confirmed by qRT-PCR and Western blot. Further bioinformatical analysis was performed to identify affected biological processes and pathways. In total, 719 genes and 28 miRNAs were downregulated, and 319 genes and 61 miRNAs were upregulated in neuroblastoma cells treated with bortezomib. Possible interactions between dysregulated miRNA/mRNA, which could be linked to bortezomib-induced neurotoxicity, affect neurogenesis, cellular calcium transport, and neuron death. Bortezomib might exert toxic effects on neuroblastoma cells and regulate miRNA–mRNA interactions influencing vital cellular functions. Further studies on the role of specific miRNA–mRNA interactions are needed to elucidate mechanisms of bortezomib action.

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“…Recently, miR-1303 was reported to function as an oncogene in numerous types of tumors. For example, Li et al suggested miR-1303 induced neuroblastoma proliferation via targeting glycogen synthase kinase 3 β and secreted frizzled related protein 1 (15). Furthermore, miR-1303 overexpression may promote the metastasis and growth of gastric cancer cells by inhibiting claudin 18 expression (16).…”

Section: Discussionmentioning

confidence: 99%

“…miR-130a can function as an oncogenic miRNA to promote cell survival and tumor growth through targeting PTEN (14). Previous, studies have also suggested that miR-1303 is related to the tumorigenesis and development of several cancers including neuroblastoma, gastric cancer and colorectal cancer (15–17); however, there are no reports of the roles of miR-1303 in PCa.…”

Section: Introductionmentioning

confidence: 99%

miR‑1303 promotes the proliferation, migration and invasion of prostate cancer cells through regulating the Wnt/β‑catenin pathway by targeting DKK3

et al. 2019

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MicroRNA-1303 (miR-1303) is involved in the tumorigenesis and progression of several cancers, and yet the role of miR-1303 in prostate cancer (PCa) and its underlying mechanism are unknown. To explore this issue, the present study aimed to use PCa tissues, cell lines and a PCa-engrafted mouse model to determine the expression and roles of miR-1303 in PCa. Furthermore, a series of experiments were conducted to explore the underlying mechanisms of action of miR-1303 in PCa cells. miR-1303 was demonstrated to be highly expressed in PCa tissues and cell lines. The level of miR-1303 expression was closely associated with higher Gleason scores and a more developed tumor stage in patients with PCa, and patients with higher levels of miR-1303 displayed a reduced overall survival rate. miR-1303 overexpression promoted the proliferation, migration and invasion of PCa cells. In vivo experiments showed that miR-1303 inhibition suppressed the growth of PCa tumors in mice. Additionally, dickkopf Wnt signaling pathway inhibitor 3 (DKK3) was identified as a target of miR-1303. Knockdown of miR-1303 suppressed the proliferation, migration and invasion of PCa cells, increased DKK3 expression, and inhibited the activity of the Wnt/β-catenin pathway. In conclusion, miR-1303 may promote proliferation, migration and invasion of PCa cells through activating the Wnt/β-catenin pathway by regulating DKK3 expression. These results indicated that miR-1303 may be considered as a potential biomarker for PCa treatment.

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miR-1303 promotes the proliferation of neuroblastoma cell SH-SY5Y by targeting GSK3β and SFRP1

Cited by 34 publications

References 18 publications

Analysis of change in microRNA expression profiles of lung cancer A549 cells treated with Radix tetrastigma hemsleyani flavonoids

Analysis of change in microRNA expression profiles of lung cancer A549 cells treated with Radix tetrastigma hemsleyani flavonoids

Molecular Mechanisms of Bortezomib Action: Novel Evidence for the miRNA–mRNA Interaction Involvement

miR‑1303 promotes the proliferation, migration and invasion of prostate cancer cells through regulating the Wnt/β‑catenin pathway by targeting DKK3

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