miR-129b suppresses cell proliferation in the human lung cancer cell lines A549 and H1299

Abstract

ABSTRACT. Lung cancer is one of the most prevalent malignant tumors, and is one of the primary causes of cancer-associated deaths. In 2002, an estimated 1.18 million lung cancer-associated deaths were recorded, accounting for 18% of cancer-related deaths and 2% of total mortality. Despite the great progress that has been made in lung cancer therapies, the mechanisms underlying lung cancer formation and development remain largely unknown. Meanwhile, the microRNA miR-129 has been shown to be involved in the formation of many types of cancer. Therefore, this study aims to investigate whether miR129b could suppress proliferation of lung cancer cell lines. NSCLC tissue samples were collected from the Department of Respiratory Medicine between April 2013 and December 2015. Ten normal health individuals were recruited as controls. Lung cancer cell lines A549 and H1299 were used to examine the suppressive effects of miR129b. Quantitative real-time PCR was used to detect miR129b expression. The MTT assay was used to analyze cell proliferation. Results indicated that miR129b is down-regulated in lung cancer cell lines and NSCLC tissues. Furthermore, overexpression of miR-129b inhibited proliferation of lung cancer cells. In conclusion, miR-129b suppresses lung cancer cell proliferation, and can be a potential therapeutic target for treatment of lung cancers.

MicroRNA-647 promotes the therapeutic effectiveness of argon–helium cryoablation and inhibits cell proliferation through targeting TRAF2 via the NF-κB signaling pathway in non-small cell lung cancer

“…Aberrant expression of miRNAs influences tumorigenic phenotypes, including cell proliferation, the cell cycle, migration, and invasion, thereby accelerating the development of cancer. In NSCLC, aberrant expression of miRNAs is a key regulatory factor underlying carcinogenicity, with miR-204, 14 miR-129b, 15 miR-1298, 16 miR-107, 17 miR-342-3p, 18 and miR-373 19 all having been implicated in previous reports. Here, we demonstrated that miR-647 was highly expressed in tumors that had undergone argon–helium cryoablation treatment, compared with those that had undergone non-argon–helium cryoablation.…”

miR‐129‐5p inhibits proliferation, migration, and invasion in rectal adenocarcinoma cells through targeting E2F7

“…Moreover, the inhibition of miR‐129 could promote tumor formation. For instance, it has been shown that the reduction of miR‐129 could contribute to human lung cancer cell proliferation (Zheng, Qi, Liu, Shi, & Yang, 2016). Therefore, we concluded that miR‐129 may have a significant influence in tumorigenesis, and its roles in READ need to be investigated.…”

MiR‐129‐5p inhibits glioma cell progression in vitro and in vivo by targeting TGIF2

“…8 MiR-129-5p is an essential member of miR-129 family. 9 Dysregulation of miR-129 family members has been investigated in various cancers such as human prostate carcinoma, 10 breast cancer, 11 lung cancer, 12 gastric cancer. 13 targeting HMGB1 was reported by Yang et al 15 Xu et al also reported that miR-129-5p inhibited glioblastoma cell viability and metastasis by targeting FNDC3B.…”