MiR-125b orchestrates cell proliferation, differentiation and migration in neural stem/progenitor cells by targeting Nestin

Cui, Yi; Xiao, Zhifeng; Han, Jin Soo; Sun, Jie; Ding, Wenyong; Zhao, Yannan; Chen, Bing; Li, Xiaoran; Dai, Jianwu

doi:10.1186/1471-2202-13-116

Cited by 65 publications

(53 citation statements)

References 30 publications

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“…miRNA-181a is rich in mature nerve cells and bioinformatic analysis has revealed that the cAMP response element-binding protein 1 (CREB1) mRNA 3'-UTR contains a complementary sequence to the miR-181a seed region. CREB1 is a key nuclear factor highly expressed in hippocampal neurons on which numerous signaling pathways converge (32)(33)(34). In the present study, the expression levels of these miRNAs were found to be significantly affected by sevoflurane and/or propofol anesthesia; therefore, general anesthesia may affect these cell signaling pathways, including synaptic plasticity and memory consolidation.…”

Section: Discussionmentioning

confidence: 49%

Changes in the gene expression levels of microRNAs in the rat hippocampus by sevoflurane and propofol anesthesia

Goto

Hori

Ishikawa

et al. 2014

Molecular Medicine Reports

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Abstract. General anesthesia is commonly used in the surgical arena, but little is known regarding its influence at the genomic and molecular levels. MicroRNAs (miRNAs) belong to a new class of non-coding RNA molecules which influence cell biology. In the present study, it was hypothesized that miRNAs alter gene expression levels under general anesthesia. The aim was to compare the miRNA expression profiles in the rat hippocampus in response to anesthesia with representative volatile (sevoflurane) and intravenous (propofol) anesthetics. Wistar Rats were randomly assigned to either a 2.4% sevoflurane, 600 µg/kg/min propofol or control (without anesthetics) group. Total RNA from hippocampal samples which contained miRNA was subjected to quantitative reverse transcriptionpolymerase chain reaction and Taqman Low-Density Arrays (TLDA). A total of 373 miRNAs are associated with rats and the TLDA analysis revealed that 279 expressed miRNAs (74.8%) were expressed in all three groups. Significant differences in the levels of 33 of the 279 expressed miRNAs (11.8%) were observed among the three groups in response to the anesthetic agents, and when compared with the control group, significant differences were found in 26 of the 279 expressed miRNAs (9.3%). Following sevoflurane anesthesia, the levels of four miRNAs were significantly increased and those of 12 were significantly reduced. By contrast, following propofol anesthesia, the levels of 11 miRNAs were significantly reduced but no miRNAs exhibited significantly elevated levels. Fourteen miRNAs were significantly differentially expressed between the two anesthesia groups. In conclusion, sevoflurane and propofol exerted different effects on miRNA expression in the rat hippocampus.

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Section: Discussionmentioning

confidence: 49%

Changes in the gene expression levels of microRNAs in the rat hippocampus by sevoflurane and propofol anesthesia

Goto

Hori

Ishikawa

et al. 2014

Molecular Medicine Reports

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“…MiR-125b target NES gene in neural stem cell [46] and it can target NES gene in SH-SY5Y cells also (Data not shown). Downregulation of NES gene after DC and RA treatment may be due to the combined effect of miR-432 and miR-125b.…”

Section: Discussionmentioning

confidence: 93%

MicroRNA‐432 contributes to dopamine cocktail and retinoic acid induced differentiation of human neuroblastoma cells by targeting NESTIN and RCOR1 genes

Das

Bhattacharyya

2014

FEBS Letters

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Edited by Tamas Dalmay Keywords:MicroRNA-432 NESTIN RCOR1 MECP2 Neurite outgrowth SH-SY5Y cells a b s t r a c t MicroRNA (miRNA) regulates expression of protein coding genes and has been implicated in diverse cellular processes including neuronal differentiation, cell growth and death. To identify the role of miRNA in neuronal differentiation, SH-SY5Y and IMR-32 cells were treated with dopamine cocktail and retinoic acid to induce differentiation. Detection of miRNAs in differentiated cells revealed that expression of many miRNAs was altered significantly. Among the altered miRNAs, human brain expressed miR-432 induced neurite projections, arrested cells in G0-G1, reduced cell proliferation and could significantly repress NESTIN/NES, RCOR1/COREST and MECP2. Our results reveal that miR-432 regulate neuronal differentiation of human neuroblastoma cells.

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“…Furthermore, several other miRs have also been revealed to mediate the proliferation of neural cells. The overexpression of miR-125b, for example, inhibited the proliferation of neural stem/progenitor cells (22), and miR-34a was revealed to enhance cell proliferation and function of newly generated neurons, as well as improve behavioral outcomes (23). Furthermore, as patients with AD are characterized by learning and memory deficits (24), SAMP8 mice were used to investigate the effect of miR-135b on learning and memory behaviors in the present study.…”

Section: Discussionmentioning

confidence: 95%

MicroRNA-135b has a neuroprotective role via targeting of β-site APP-cleaving enzyme 1

Zhang

Xing

Guo

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. MicroRNAs (miRs) are a class of endogenous small non-coding RNAs that have been revealed to negatively mediate the expression of their target genes at the post-transcriptional level. Recently, particular miRs have demonstrated an involvement in the pathogenesis of Alzheimer's disease (AD). However, the specific role of miR-135b in AD has yet to be elucidated. The present study aimed to investigate the neuroprotective role of miR-135b, in addition to its underlying mechanism. Herein, reverse transcription-quantitative polymerase chain reaction was conducted to determine miR-135b expression levels in the peripheral blood samples of patients with AD and age-matched normal controls. The data of the present study revealed that the expression levels of miR-135b were significantly reduced in the peripheral blood of AD patients compared with normal controls (P<0.01). In vitro MTT analyses identified that the overexpression of miR-135b significantly enhanced the proliferation of hippocampal cells (P<0.01). Furthermore, in vivo analysis using a Y-maze test indicated that injection with miR-135b mimics into the third ventricle of anesthetized SAMP8 mice significantly enhanced their learning and memory capacities (P<0.01). Molecular mechanism investigations identified β-site APP-cleaving enzyme 1 (BACE1) as a direct target gene of miR-135b, and the latter was identified to negatively mediate the protein expression levels of BACE1 in hippocampal cells, in addition to hippocampal tissues, of SAMP8 mice. Based on the aforementioned findings, we propose that miR-135b has a neuroprotective role via direct targeting of BACE1 and, thus, may be used for the treatment of AD.

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MiR-125b orchestrates cell proliferation, differentiation and migration in neural stem/progenitor cells by targeting Nestin

Cited by 65 publications

References 30 publications

Changes in the gene expression levels of microRNAs in the rat hippocampus by sevoflurane and propofol anesthesia

Changes in the gene expression levels of microRNAs in the rat hippocampus by sevoflurane and propofol anesthesia

MicroRNA‐432 contributes to dopamine cocktail and retinoic acid induced differentiation of human neuroblastoma cells by targeting NESTIN and RCOR1 genes

MicroRNA-135b has a neuroprotective role via targeting of β-site APP-cleaving enzyme 1

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