MicroRNA‐432 contributes to dopamine cocktail and retinoic acid induced differentiation of human neuroblastoma cells by targeting NESTIN and RCOR1 genes

Das, Eashita; Bhattacharyya, Nitai P.

doi:10.1016/j.febslet.2014.03.015

Cited by 39 publications

(32 citation statements)

References 53 publications

(66 reference statements)

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“…25,26 Nestin, as an important stem cell/progenitor cell biomarker, is crucial for the mitogen-stimulated proliferation of neural progenitor cells, and loss or lack of it would lead to apoptosis of neural progenitor cells in zebrafish. 27 Das et al 28 has suggested that miR-432 contributes to dopamine cocktail and retinoic acid-induced differentiation of human neuroblastoma cells by targeting NESTIN and RCOR1 genes, and miR-432 could meditate the process of neuronal differentiation in human neuroblastoma cells, which supports our results. Consequently, we found that compared with the blank and NC groups, frequency of social behaviors of juvenile Wistar rats in the si-C21orf121, miR-140-5p mimic, and si-C21orf121 + miR-140-5p mimic decreased, but accumulated time of repetitive stereotyped movements increased.…”

Section: Discussionsupporting

confidence: 91%

“…Nestin, as an important stem cell/progenitor cell biomarker, is crucial for the mitogen‐stimulated proliferation of neural progenitor cells, and loss or lack of it would lead to apoptosis of neural progenitor cells in zebrafish . Das et al has suggested that miR‐432 contributes to dopamine cocktail and retinoic acid–induced differentiation of human neuroblastoma cells by targeting NESTIN and RCOR1 genes, and miR‐432 could meditate the process of neuronal differentiation in human neuroblastoma cells, which supports our results.…”

Section: Discussionsupporting

confidence: 89%

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Long noncoding RNA C21orf121/bone morphogenetic protein 2/microRNA‐140‐5p gene network promotes directed differentiation of stem cells from human exfoliated deciduous teeth to neuronal cells

Zhang

et al. 2018

J of Cellular Biochemistry

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Previous studies have revealed that long noncoding RNA (lncRNA) and microRNA play a crucial role in autism, which is a childhood neurodevelopmental disorder with complicated genetic origins. Hence, the study concerns whether lncRNA C21orf121/bone morphogenetic proteins 2 (BMP2)/miR‐140‐5p gene network affects directed differentiation of stem cells from human exfoliated deciduous teeth (SHED) to neuronal cells in rats with autism. Autism models were successfully established. The neuron cells that differentiated from SHED cell were identified. The expression of lncRNA C21orf121, miR‐140‐5p, BMP2, Nestin, βIII‐tubulin, and microtubule‐associated protein 2 (MAP2) and the expression of neuron‐specific enolase (NSE) were examined. Besides, the gap junction (GJ) function of SHED, the intracellular free Ca 2+ concentration, and the social behavior and repetitive stereotyped movements of rats in autism were detected. The target relationship between lncRNA C21orf121 and miR‐140‐5p and that between miR‐140‐5p and BMP2 were also verified. Firstly, we successfully isolated SHED and identified the differentiated neurons of SHED. Besides, the expression of BMP2, MAP2, Nestin, βIII‐tubulin, NSE positive rate, GJ function, and intracellular free Ca 2+ concentration were increased with the upregulation of C21orf121 and downregulation of miR‐140‐5p, and accumulated time of repetitive stereotyped movements decreased and the frequency of social behavior increased. The results indicate that lncRNA C21orf121 as a competing endogenous RNA competes with BMP2 binding to miR‐140‐5p, thereby promoting SHED to differentiate into neuronal cells via upregulating BMP2 expression.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 89%

Long noncoding RNA C21orf121/bone morphogenetic protein 2/microRNA‐140‐5p gene network promotes directed differentiation of stem cells from human exfoliated deciduous teeth to neuronal cells

Zhang

et al. 2018

J of Cellular Biochemistry

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“…The neuronal cells adhere weakly to the substrate and grow as clumps of cells. For instance, SH-SY5Y, a neuroblastoma cell line, is an in vitro model for investigating the early stages of neuronal differentiation and neural tissue engineering strategies4567. Differentiation of SH-SY5Y induced pharmacologically has also been studied as a model of dopaminergic neurons for Parkinson's disease research8.…”

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confidence: 99%

Probing Mechanoregulation of Neuronal Differentiation by Plasma Lithography Patterned Elastomeric Substrates

Nam

Jamilpour

Mfoumou

et al. 2014

Sci Rep

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Cells sense and interpret mechanical cues, including cell-cell and cell-substrate interactions, in the microenvironment to collectively regulate various physiological functions. Understanding the influences of these mechanical factors on cell behavior is critical for fundamental cell biology and for the development of novel strategies in regenerative medicine. Here, we demonstrate plasma lithography patterning on elastomeric substrates for elucidating the influences of mechanical cues on neuronal differentiation and neuritogenesis. The neuroblastoma cells form neuronal spheres on plasma-treated regions, which geometrically confine the cells over two weeks. The elastic modulus of the elastomer is controlled simultaneously by the crosslinker concentration. The cell-substrate mechanical interactions are also investigated by controlling the size of neuronal spheres with different cell seeding densities. These physical cues are shown to modulate with the formation of focal adhesions, neurite outgrowth, and the morphology of neuroblastoma. By systematic adjustment of these cues, along with computational biomechanical analysis, we demonstrate the interrelated mechanoregulatory effects of substrate elasticity and cell size. Taken together, our results reveal that the neuronal differentiation and neuritogenesis of neuroblastoma cells are collectively regulated via the cell-substrate mechanical interactions.

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“…A recent report from our laboratory showed that hsa-miR-432 can induce neuronal differentiation in human neuroblastoma cells [29] . However, how hsa-miR-432 expression is regulated remained unknown.…”

Section: Sprague-dawley Ratsmentioning

confidence: 97%

Widening HSF1 horizon: the microRNA connection

Das

Bhattacharyya

2015

RNA Dis

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Heat shock factor 1 (HSF1) is an evolutionary conserved transcription factor which acts as protector of cellular proteome against adverse environmental conditions including increased temperature in eukaryotes. HSF1 positively regulates expression of a set of cytoprotective proteins popularly called as heat shock proteins (HSPs) as part of an adaptive mechanism of cells known as heat shock response (HSR). It has also been shown that apart from cellular stress response, HSF1 has the ability to regulate many other biological processes including aging, metabolism, development etc. This is primarily achieved by the ability of HSF1 to regulate expression of myriad of genes other than those coding for classical HSPs in presence and/or absence of stress. MicroRNAs (miRNAs) are short endogenous RNA molecules which can regulate many biological processes by acting as post-transcriptional regulators of gene expression. Altered expression of miRNAs in response to thermal stress has been reported in different species; however, the underlying mechanism remained elusive. We recently showed that HSF1 has the ability to regulate expression of miRNAs by binding to their upstream sequences and our result established hsa-miR-432 as the first HSF1-regulated miRNA.We therefore speculate that the landscape of HSF1 transcripts is much broader than it was earlier thought and includes HSF1-regulated miRNAs, the recently identified non-coding arm of HSF1 transcripts. The HSF1-regulated miRNAs have the potential to regulate many cellular events by acting as important downstream molecules of HSF1.Our finding thus uncovers a novel functional aspect of HSF1 with the emergence of hsa-miR-432 as a novel transcriptional target of HSF1.

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MicroRNA‐432 contributes to dopamine cocktail and retinoic acid induced differentiation of human neuroblastoma cells by targeting NESTIN and RCOR1 genes

Cited by 39 publications

References 53 publications

Long noncoding RNA C21orf121/bone morphogenetic protein 2/microRNA‐140‐5p gene network promotes directed differentiation of stem cells from human exfoliated deciduous teeth to neuronal cells

Long noncoding RNA C21orf121/bone morphogenetic protein 2/microRNA‐140‐5p gene network promotes directed differentiation of stem cells from human exfoliated deciduous teeth to neuronal cells

Probing Mechanoregulation of Neuronal Differentiation by Plasma Lithography Patterned Elastomeric Substrates

Widening HSF1 horizon: the microRNA connection

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