Probing Mechanoregulation of Neuronal Differentiation by Plasma Lithography Patterned Elastomeric Substrates

Nam, Ki Hwan; Jamilpour, Nima; Mfoumou, Etienne; Wang, Fei-Yue; Zhang, Donna D.; Wong, Pak Kin

doi:10.1038/srep06965

Cited by 27 publications

(23 citation statements)

References 46 publications

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“…Further, the microtraps allow soma motility and possibility of positioning at different points inside the microtraps. Restriction on soma at gap of < 50–60 µm from the immediate topography allows both freedom of navigation with a possibility of sensing the boundary profile of the confinements, since with greater distance from the boundaries, there is a possibility of reduction in cellular stresses …”

Section: Discussionmentioning

confidence: 99%

Geometrically Mediated Topographic Steering of Neurite Behaviors and Network Formation

Kuddannaya

Tong

Fan

et al. 2018

Adv Materials Inter

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The relationship between different mechanoregulatory factors and mechanisms by which neuronal development is regulated by diverse biophysical cues in the immediate vicinity of neural cells remains sparsely understood. Considering the extreme physical complexity of neuronal niches, it is imperative to look beyond the chemical cues for a holistic understanding in these lines. Here the role of geometrically diverse physical microcues is studied in morphologically regulating neurite branching, direction, growth, and network formation in nascent primary cortical neurons on a simple cytocompatible interface. The results indicate distinct differences in neurite branching, microgap sensing, and path‐finding tendencies in response to the underlying angled/curved cues on the micropatterned interface. While the microtraps along curved cues promote higher branching, the highly angled cues promote relatively lower branching with higher neurite lengths. In this study specific morphological changes induced by these cues on the collective conformity, branching, and mean length of neurites in both isolated neurons and neurons in networks are investigated. Finally, a model is proposed to study mechanoregulation of isolated neurons and neuronal networks in response to diverse physical cues. Overall, this platform aims to propel efficient morphological modulation for the study of neurodevelopmental anomalies or physical guidance inspired repair strategies.

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Section: Discussionmentioning

confidence: 99%

Geometrically Mediated Topographic Steering of Neurite Behaviors and Network Formation

Kuddannaya

Tong

Fan

et al. 2018

Adv Materials Inter

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“…Cell morphology. Recent NTE studies have investigated cell proliferation and differentiation on aligned fiber substrates, 11,13,20 and have highlighted that 3D topography generates cues that mimic the neural environment, inducing morphological as well as functional changes for cells. 20,55 We investigated cell morphology changes following NSC culture and differentiation on PVDF scaffolds, using SEM.…”

Section: Scaffold Architecturesmentioning

confidence: 99%

“…On the other hand, the glial cells—such as oligodendrocytes, microglia, and astrocytes—provide a mechanical support as well as a favorable medium for nervous signal transmission. Biophysicists have studied the mechanoregulation of neuronal differentiation on model‐patterned substrates to propose optimized scales and geometries of three‐dimensional (3D) scaffolds and circuits, as well as theoretical models …”

Section: Introductionmentioning

confidence: 99%

“…Biophysicists have studied the mechanoregulation of neuronal differentiation on model-patterned substrates to propose optimized scales and geometries of three-dimensional (3D) scaffolds and circuits, as well as theoretical models. [10][11][12] Several authors demonstrated that patterned substrates could be used to guide the in vitro growth and differentiation of embryonic and mesenchymal stem cells toward a neurogenic lineage. 13 Different methods have been developed to process 3D scaffolds for NTE, including selfassembly, phase separation, patterning from lithography or nano-objects, 3D printing, melt spinning, and electrospinning.…”

Section: Introductionmentioning

confidence: 99%

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Effect of polyvinylidene fluoride electrospun fiber orientation on neural stem cell differentiation

et al. 2016

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Electrospun polymer piezoelectric fibers can be used in neural tissue engineering (NTE) to mimic the physical, biological, and material properties of the native extracellular matrix. In this work, we have developed scaffolds based on polymer fiber architectures for application in NTE. To study the role of such three-dimensional scaffolds, a rotating drum collector was used for electrospinning poly(vinylidene) fluoride (PVDF) polymer at various rotation speeds. The morphology, orientation, polymorphism, as well as the mechanical behavior of the nonaligned and aligned fiber-based architectures were characterized. We have demonstrated that the jet flow and the electrostatic forces generated by electrospinning of PVDF induced local conformation changes which promote the generation of the β-phase. Fiber anisotropy could be a critical feature for the design of suitable scaffolds for NTEs. We thus assessed the impact of PVDF fiber alignment on the behavior of monkey neural stem cells (NSCs). NSCs were seeded on nonaligned and aligned scaffolds and their morphology, adhesion, and differentiation capacities into the neuronal and glial pathways were studied using microscopic techniques. Significant changes in the growth and differentiation capacities of NSCs into neuronal and glial cells as a function of the fiber alignment were evidenced. These results demonstrate that PVDF scaffolds may serve as instructive scaffolds for NSC survival and differentiation, and may be valuable tools for the development of cell- and scaffold-based strategies for neural repair. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2376-2393, 2017.

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“…In the assay, the geometry of the cell monolayer is controlled by spatially patterning the hydrophobicity of the substrate and collective migration is induced by removing a physical blocker to create a cell free region in the pattern 18 . The plasma lithography technique has been previously demonstrated for investigating several biological systems 19 20 21 . The formation of leader cells and the migration rate of the monolayer are investigated in rectangular patterns of various dimensions.…”

mentioning

confidence: 99%

Probing Leader Cells in Endothelial Collective Migration by Plasma Lithography Geometric Confinement

Yang

Jamilpour

Yao

et al. 2016

Sci Rep

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When blood vessels are injured, leader cells emerge in the endothelium to heal the wound and restore the vasculature integrity. The characteristics of leader cells during endothelial collective migration under diverse physiological conditions, however, are poorly understood. Here we investigate the regulation and function of endothelial leader cells by plasma lithography geometric confinement generated. Endothelial leader cells display an aggressive phenotype, connect to follower cells via peripheral actin cables and discontinuous adherens junctions, and lead migrating clusters near the leading edge. Time-lapse microscopy, immunostaining, and particle image velocimetry reveal that the density of leader cells and the speed of migrating clusters are tightly regulated in a wide range of geometric patterns. By challenging the cells with converging, diverging and competing patterns, we show that the density of leader cells correlates with the size and coherence of the migrating clusters. Collectively, our data provide evidence that leader cells control endothelial collective migration by regualting the migrating clusters.

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Probing Mechanoregulation of Neuronal Differentiation by Plasma Lithography Patterned Elastomeric Substrates

Cited by 27 publications

References 46 publications

Geometrically Mediated Topographic Steering of Neurite Behaviors and Network Formation

Geometrically Mediated Topographic Steering of Neurite Behaviors and Network Formation

Effect of polyvinylidene fluoride electrospun fiber orientation on neural stem cell differentiation

Probing Leader Cells in Endothelial Collective Migration by Plasma Lithography Geometric Confinement

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