miR-122 dysregulation is associated with type 2 diabetes mellitus-induced dyslipidemia and hyperglycemia independently of its rs17669 variant

Abstract

Background: miR-122 is a liver speci c micro RNA that participates in the regulation of carbohydrate and lipid metabolism. rs17669 variant positioned at the anking region of miR-122 and may be affects its stability and maturation. Therefore, this study was aimed to investigates the association between rs17669 polymorphism with the miR-122 circulating level, risk of type 2 diabetes mellitus (T2DM) development and biochemical parameters in these patients and matched healthy controls.Methods and Results: Two hundred and ninety-ve subjects (controls; n=145 and T2DM; n=150) enrolled in this study. rs17669 variant genotyping was done by ARMS-PCR. Serum biochemical parameters including lipid pro le and glucose were measured by colorimetric kits. Insulin and Glycated hemoglobin (HbA1c) were assayed by using capillary electrophoresis and ELISA methods respectively. miR-122 expression was measured by real-time PCR. There was no signi cant difference in allele and genotype distribution between study groups (P>0.05). rs17669 variant has not any signi cant association with miR-122 gene expression and biochemical parameters (P>0.05). miR-122 expression level in T2DM patients was considerably higher than that in control subjects (5.7±2.4 vs 1.4±0.78) (P>0.05). Furthermore, there were a positive and signi cant correlation between miR-122 fold change with LDL-C, sdLDL, FBS and insulin resistance (P>0.05).Conclusions: It can be concluded that miR-122 rs17669 variant is not associated to the miR-122 expression and T2DM-associated serum parameters. Furthermore, it can be suggesting that miR-122 dysregulation involved in T2DM development through the inducing dyslipidemia, hyperglycemia as well as resistance to insulin.

Correlation between serum microRNA-122 and VEGF expression and pregnancy outcome in gestational diabetes mellitus patients

“…Consistent with our findings, Abnoos Mokhtari Ardekani et al indicate that miR-122 expression level in type 2 diabetes mellitus (T2DM) patients is significantly higher than that in control subjects. 36 Moreover, miR-122 is a potential novel tool for the early diagnosis and risk estimation of non-alcoholic fatty liver disease in T2DM patients. 37 …”

“…Consistent with our findings, Abnoos Mokhtari Ardekani et al indicate that miR-122 expression level in type 2 diabetes mellitus (T2DM) patients is significantly higher than that in control subjects. 36 Moreover, miR-122 is a potential novel tool for the early diagnosis and risk estimation of non-alcoholic fatty liver disease in T2DM patients. 37 In addition, the outcomes of this study demonstrated that the total incidence of adverse pregnancy outcomes in the RG was significantly higher than the CG, and the levels of miR-122 and VEGF in the poor outcome group were elevated relative to the good outcome group.…”