miR-107 inhibits CDK6 expression, differentiation, and lipid storage in human adipocytes

Ahonen, Maria A.; Haridas, Parvathi; Mysore, Raghavendra; Wabitsch, Martin; Fischer‐Posovszky, Pamela; Olkkonen, Vesa M.

doi:10.1016/j.mce.2018.09.007

Cited by 35 publications

(33 citation statements)

References 36 publications

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“…On the basis of target prediction using bioinformatics analyses, HOTAIRM1 may serve as a ceRNA for miR-107. Previous studies have demonstrated that miR-107 is engaged in numerous biological processes, including cell differentiation 22 , response to chemotherapy 23 , insulin resistance 24 , and metastasis 25,26 . Accumulating evidence has shown that high levels of miR-107 may be a risk factor in the prognostic monitoring of malignant diseases, such as gastric cancer 27 , oropharyngeal cancer 28 , colorectal cancer 29 , and breast cancer 30 .…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: The HOTAIRM1/miR-107/TDG axis regulates papillary thyroid cancer cell proliferation and invasion

Chai

et al. 2020

Cell Death Dis

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The long noncoding RNA (lncRNA), HOX antisense intergenic RNA myeloid 1 (HOTAIRM1), has been shown to act as a tumor suppressor in various human cancers. However, the overall biological roles and clinical significance of HOTAIRM1 in papillary thyroid cancer (PTC) have not been investigated. In this study, we used quantitative reverse transcription PCR (qRT-PCR) to show that HOTAIRM1 was significantly downregulated in PTC tissues and low HOTAIRM1 expression levels were associated with lymph node metastasis and advanced TNM stage. We performed Cell Counting Kit-8, plate colony-formation, flow cytometric apoptosis, transwell, and scratch wound healing assays. Overexpression of HOTAIRM1 was found to inhibit PTC cell proliferation, invasion, and migration in vitro. Additionally, we identified miR-107 as a target of HOTAIRM1 using online bioinformatics tools. Dual-luciferase reporter gene and RNA immunoprecipitation assays were used to confirm that HOTAIRM1 acted as a competing endogenous RNA of miR-107. Furthermore, enhancement of miR-107 could potentially reverse the effects of HOTAIRM1 overexpression in vitro. Inhibition of miR-107 suppressed PTC cell proliferation, invasion, and migration in vitro. HOTAIRM1 overexpression and miR-107 inhibition impaired tumorigenesis in vivo in mouse xenografts. Bioinformatics prediction and a dual-luciferase reporter gene assay demonstrated the binding between miR-107 and the 3′-untranslated region of TDG. The results of qRT-PCR and western blotting assays suggested that HOTAIRM1 could regulate the expression of TDG in an miR-107meditated manner. In conclusion, we validated HOTAIRM1 as a novel tumor-suppressor lncRNA in PTC and proposed that the HOTAIRM1/miR-107/TDG axis may serve as a therapeutic target for PTC.

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: The HOTAIRM1/miR-107/TDG axis regulates papillary thyroid cancer cell proliferation and invasion

Chai

et al. 2020

Cell Death Dis

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“…These miRNAs target important thermogenic genes with either positive or negative impact on the BAT thermogenic program [ 71 , 80 ]. Moreover, various miRNAs, such as the MiR-193b-365 cluster, MiR-328, MiR-378, MiR-30b/c, MiR-455, and MiR-32, are activators of brown adipogenesis, whereas MiR-34a, MiR-133, MiR-155, and MiR-27b are brown adipogenesis inhibitors ( Table 1 and Figure 2 ) [ 81 , 82 , 83 , 84 ]. Manipulation of these miRNAs has been shown to affect uncoupled respiration, glucose uptake, and thermogenesis, as well as whole-body energy expenditure, glucose tolerance, and insulin sensitivity [ 6 , 71 , 80 ].…”

Section: Micrornas In Batmentioning

confidence: 99%

Regulatory microRNAs in Brown, Brite and White Adipose Tissue

Gharanei

Shabir

Brown

et al. 2020

Cells

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MicroRNAs (miRNAs) constitute a class of short noncoding RNAs which regulate gene expression by targeting messenger RNA, inducing translational repression and messenger RNA degradation. This regulation of gene expression by miRNAs in adipose tissue (AT) can impact on the regulation of metabolism and energy homeostasis, particularly considering the different types of adipocytes which exist in mammals, i.e., white adipocytes (white AT; WAT), brown adipocytes (brown AT; BAT), and inducible brown adipocytes in WAT (beige or brite or brown-in-white adipocytes). Indeed, an increasing number of miRNAs has been identified to regulate key signaling pathways of adipogenesis in BAT, brite AT, and WAT by acting on transcription factors that promote or inhibit adipocyte differentiation. For example, MiR-328, MiR-378, MiR-30b/c, MiR-455, MiR-32, and MiR-193b-365 activate brown adipogenesis, whereas MiR-34a, MiR-133, MiR-155, and MiR-27b are brown adipogenesis inhibitors. Given that WAT mainly stores energy as lipids, whilst BAT mainly dissipates energy as heat, clarifying the effects of miRNAs in different types of AT has recently attracted significant research interest, aiming to also develop novel miRNA-based therapies against obesity, diabetes, and other obesity-related diseases. Therefore, this review presents an up-to-date comprehensive overview of the role of key regulatory miRNAs in BAT, brite AT, and WAT.

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“…Early life stress and inflammation may have a huge impact on the development of chronic pain and is reported in several studies among FMS patients [ 38 , 39 ]. MiR107 is associated with childhood traumatization [ 15 ] and plays a regulatory role in inflammation [ 16 , 17 ] by targeting CDK and TLR4 [ 18 , 19 ]. Pro-inflammatory cytokines i.e.…”

Section: Discussionmentioning

confidence: 99%

“…the STAT6/IL4 anti-inflammatory pathway [ 13 ] or the SNRK / NF-κB / p65 signaling pathway [ 14 ]. MiR107 is linked to childhood trauma [ 15 ] and has a regulatory role in inflammation [ 16 , 17 ] by targeting cycline dependent kinases (CDK) and toll like receptors (TLR) [ 18 , 19 ]. Activated TLR4 was shown to induce secretion of tumor necrosis factor-alpha (TNF) in patients with renal sepsis [ 20 ] and to attenuate adaptive thermogenesis in obese mice via endoplasmatic reticulum stress.…”

Section: Introductionmentioning

confidence: 99%

MiR103a-3p and miR107 are related to adaptive coping in a cluster of fibromyalgia patients

et al. 2020

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Background MicroRNA (miRNA) mainly inhibit post-transcriptional gene expression of specific targets and may modulate disease severity. Objective We aimed to identify miRNA signatures distinguishing patient clusters with fibromyalgia syndrome (FMS). Subjects and methods We previously determined four FMS patient clusters labelled “maladaptive”, “adaptive”, “vulnerable”, and “resilient”. Here, we cluster-wise assessed relative gene expression of miR103a-3p, miR107, miR130a-3p, and miR125a-5p in white blood cell (WBC) RNA of 31 FMS patients and 16 healthy controls. Sum scores of pain-, stress-, and resilience-related questionnaires were correlated with miRNA relative gene expression. A cluster-specific speculative model of a miRNA-mediated regulatory cycle was proposed, and its potential targets verified by the online tool “ target scan human” . Results One-way ANOVA revealed lower gene expression of miR103a-3p, miR107, and miR130a-3p in FMS patients compared to controls (p < 0.05). Follow-up post-hoc tests indicated the highest peak of gene expression of miR103a-3p for the adaptive cluster (p < 0.05), i.e. in patients with low disability in all symptom categories. Gene expression of miR103a-3p correlated with FMS related disability and miR107 with the score “physical abuse” of the trauma questionnaire (p < 0.05). Target scan identified sucrose non-fermentable serine/threonine protein kinase, nuclear factor kappa-b, cyclin dependent kinase, and toll-like receptor 4 as genetic targets of the miR103a/107 miRNA family. Conclusion We show an association between upregulated gene expression of miR103a, tendentially of miR107, and adaptive coping in FMS patients. Validation of this pair of miRNA may enable to identify a somatic resilience factor in FMS.

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miR-107 inhibits CDK6 expression, differentiation, and lipid storage in human adipocytes

Cited by 35 publications

References 36 publications

RETRACTED ARTICLE: The HOTAIRM1/miR-107/TDG axis regulates papillary thyroid cancer cell proliferation and invasion

RETRACTED ARTICLE: The HOTAIRM1/miR-107/TDG axis regulates papillary thyroid cancer cell proliferation and invasion

Regulatory microRNAs in Brown, Brite and White Adipose Tissue

MiR103a-3p and miR107 are related to adaptive coping in a cluster of fibromyalgia patients

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