MiR-106b-5p regulates the migration and invasion of colorectal cancer cells by targeting FAT4

Abstract

MicroRNA-106b-5p (miR-106b-5p) is involved in the development of many cancers including in colorectal cancer (CRC), and FAT4 is correlated with regulation of growth and apoptosis of cancer cells. This study aimed to investigate the relation between FAT4 and miR-106b-5p and the underlying mechanism of the two on the development of CRC. Quantitative real-time PCR (qRT-PCR) assay and Western blot (WB) analysis were performed to detect the expressions of mRNAs, miRNA and proteins. The viability of CRC cells was detected by cell counting kit-8 (CCK-8). Scratch test and transwell assay were performed to measure the migration and invasion of CRC cell. Tumor angiogenesis was simulated by in vitro angiogenesis experiment. Dual-luciferase reporter assay was performed to verify the targeting relation between miR-106b-5p and FAT4. The study found that the expression of FAT4 was down-regulated and that of miR-106b-5p was up-regulated in CRC tissues. Overexpression of FAT4 resulted in decreased proliferation, migration, invasion and angiogenesis of CRC cells, whereas silencing of FAT4 led to the opposite results. In rescue experiment, miR-106b-5p partially reversed the function of FAT4 in CRC cells, thus playing a carcinogenic role by targeting FAT4 in the CRC cells.

Exosomes-derived miR-548am-5p promotes colorectal cancer progression

“…According to the literature proposed by Longchang Huang et al, miR-4319 prevents CRC progression by controlling ABTB1 (18). Inversely, Min Pan et al have pointed out that miR-106b-5p accelerates CRC cell migration and invasion via binding to FAT4 (19). A former study has indicated that miR-548am-5p is differentially expressed in the pathogenesis of early-stage COAD, but the definite expression pattern is unclear.…”

Non-Coding RNAs in Colorectal Cancer: Their Functions and Mechanisms

“…Despite their physiological and morphological homogeneity, tumors display a wide range of morphological and phenotypic differences, including the expression of receptors on the surface of cells and their angiogenic capabilities ( 128 ). Angiogenesis is identified as an important hallmark of malignant tumors because quantities of blood vessels are needed to feed cancer cells ( 129 ).…”

Integrative p53, micro-RNA and Cathepsin Protease Co-Regulatory Expression Networks in Cancer

“…Cai et al (2011) reported that miRNA-106b could target RB expression in laryngeal carcinoma [ 147 ] and ATG16L1 expression in Crohn’s Disease samples [ 148 , 149 ]. Additionally, all three micro-RNAs from the miRNA-106b-25 cluster were seen to target PTEN expression [ 150 , 151 ] and increased miRNA-106b expression recorded in CRC tissues which could target DLC-1 (while enhancing EMT, [ 152 ]) and FAT4 in CRC tissues or cell lines [ 153 ], ( Table 7 ).…”