MiR-106b-5p Promotes Proliferation and Inhibits Apoptosis by Regulating BTG3 in Non-Small Cell Lung Cancer

Wei, Ke; Pan, Chunfeng; Yao, Guoliang; Liu, Bin; Ma, Teng; Xia, Yang; Jiang, Wei; Liang, Chen; Chen, Yijiang

doi:10.1159/000485650

Cited by 49 publications

(47 citation statements)

References 51 publications

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“…A previous study has shown that microRNAs are involved in multiple aspects of lung cancer such as cell proliferation, apoptosis, invasion, and EMT (3)(4)(5)(6). Additionally, the survival of lung cancer patients has been predicted by a series of microRNAs such as miR-133b, miR-93-5p (7,8).…”

Section: Introductionmentioning

confidence: 99%

miR-1323 Promotes Cell Migration in Lung Adenocarcinoma by Targeting Cbl-b and Is an Early Prognostic Biomarker

et al. 2020

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Purpose: MicroRNAs are known to regulate cellular processes in non-small cell lung cancer (NSCLC) cells and predict prognosis. However, identification of specific microRNAs in NSCLC as potential therapeutic targets is controversial. We aim to determine the clinical significance of miR-1323 in the prognosis of patients with lung cancer and the potential mechanism. Patients and methods: A bioinformatics approach was used to screen the importance microRNA in NSCLC through the online GEO database (GSE42425). The relationship between expression level of miR-1323 and overall survival of lung cancer patients was analyzed. Additionally, an independent corhort including 53 NSCLC cases that underwent resection validated the connection between miR-1323 and LUAD patients' overall survival. Next, the function of miR-1323 was studied in vitro by transient transfection. A more in-depth mechanism was studied through luciferase reporter gene experiments. Results: High miR-1323 expression correlated with poor survival in NSCLC patients (P = 0.011), and in lung adenocarcinoma (LUAD) patients (P = 0.015) based on GEO database (GSE42425). In the independent cohort based on our hospital, high miR-1323 expression was associated with LUAD patients (P = 0.025). Moreover, transfection with mimics of miR-1323 showed an increased migratory capacity in LUAD A549 and HCC827 cells. In addition, E3 ubiquitin-protein ligase Casitas B-lineage Lymphoma-b (Cbl-b) was found to be the target genes of miR-1323 and significantly down regulated after mimics of miR-1323 transfection, and high Cbl-b expression predicted better prognosis in NSCLC and LUAD (P = 0.00072 and P = 0.02, respectively). Conclusion: The miR-1323 promoted LUAD migration through inhibiting Cbl-b expression. High miR-1323 expression predicted poor prognosis in LUAD patients.

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Section: Introductionmentioning

confidence: 99%

miR-1323 Promotes Cell Migration in Lung Adenocarcinoma by Targeting Cbl-b and Is an Early Prognostic Biomarker

et al. 2020

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“…A number of miR-106b-5p target genes have been identified, including SET domain containing 2, histone lysine methyltransferase (11), cathepsin A (27), PTEN (14) and BTG anti-proliferation factor 3 (15), which have pivotal roles in anti-oncogenic processes. Since miRNAs depress the expression of their target mRNAs, the present study hypothesized that miR-106b-5p may target certain genes that function as tumor suppressors.…”

Section: Discussionmentioning

confidence: 99%

“…MiRNAs cause mRNA degradation and post-transcriptional downregulation by binding to the 3'untranslated region (UTR) of their target genes (10). miR-106b-5p, a member of the miR-106b-25 cluster, is mapped to human chromosome 7q21 (Chr7q21) locus and has been reported to be amplified in several types of human malignancies, including renal cell carcinoma (11), hepatocellular carcinoma (12), glioma (13), melanoma (14) and non-small cell lung cancer (15). The deleterious effects of miRNA dysregulation on malignant behaviors appear to be strongly associated with tumorigenesis and cell cycle progression (12)(13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

miR‑106b‑5p promotes cell proliferation and cell cycle progression by directly targeting CDKN1A in osteosarcoma

He¹,

Chen²,

Liu³

et al. 2020

Exp Ther Med

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MicroRNA (miR)-106b-5p has been reported to act as both an oncogene and tumor suppressor in several tumors. The aim of the present study was to investigate the biological function of miR-106b-5p in osteosarcoma (OS). miR-106b-5p expression was observed to be significantly increased in OS tissues and cell lines. MTT assay and flow cytometry analysis determined that miR-106b-5p inhibitor transfection suppressed OS cell proliferation and induced cell cycle G0/G1 phase arrest. Furthermore, bioinformatics analysis and a luciferase reporter assay demonstrated that cyclin-dependent kinase inhibitor 1A (CDKN1A) was a potential target of miR-106b-5p. p21 protein expression was found to be significantly increased by miR-106b-5p downregulation in OS cells. Further analysis demonstrated that CDKN1A was downregulated in OS tissues and was negatively correlated with miR-106b-5p expression. Furthermore, upregulation of CDKN1A expression mimicked, whilst CDKN1A knockdown reversed the suppressive effects of miR-106b-5p inhibitor on OS cell proliferation and cell cycle progression. In summary, the present data suggested that miR-106b-5p promotes cell proliferation and cell cycle progression by directly targeting CDKN1A in OS.

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“…Furthermore, the underlying mechanism by which NSCLC is modulated is not fully understood (25). miRNAs are key regulators of NSCLC progression and therefore may become an important therapeutic target (26). The abnormal expression of circulating miRNA has also been widely reported in patients with NSCLC (6,26).…”

Section: Discussionmentioning

confidence: 99%

“…miRNAs are key regulators of NSCLC progression and therefore may become an important therapeutic target (26). The abnormal expression of circulating miRNA has also been widely reported in patients with NSCLC (6,26). Increasing evidence has indicate the important role of miR-484 in various types of tumor (18,27,28).…”

Section: Discussionmentioning

confidence: 99%

High serum miR‑484 expression is associated with the diagnosis and prognosis of patients with non‑small cell lung cancer

2019

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The aim of the current study was to assess the expression and clinical significance of serum microRNA (miR)-484 in patients with non-small cell lung cancer (NSCLC). Reverse transcription-quantitative polymerase chain reaction was performed to determine the expression of miR-484 in the serum of patients with NSCLC and NSCLC cell lines. Cell counting kit-8, flow cytometry, cell migration and cell invasion assays were performed to assess the role of miR-484 in the malignant changes associated with NSCLC cells. Furthermore, to assess the diagnostic value of miR-484, receiver operating curve (ROC) analysis was performed and the clinical relevance of serum miR-484 expression in patients with NSCLC was determined. A Kaplan-Meier analysis with the log-rank test was performed to assess the overall survival rate patients. To the best of our knowledge, the current study demonstrates for the first time that serum miR-484 was increased in patients with NSCLC compared with healthy controls. Additionally, serum miR-484 was revealed to be positively associated with histological grade, lymph node metastasis, distant metastasis and clinical stage. Patients with NSCLC and high serum miR-484 levels demonstrated significantly poorer overall survival rates compared with those exhibiting lower serum miR-484 expressions. ROC analysis revealed that serum miR-484 could screen patients with NSCLC patients from healthy controls with a high sensitivity and specificity. In vitro analysis also demonstrated that miR-484 was significantly upregulated in NSCLC cell lines, including 95D and H358 cells. Furthermore, the suppression of miR-484 decreased cell proliferation, cell migration and invasion. In summary, the results of the present study demonstrated that increased serum miR-484 expression is associated with the diagnosis and prognosis of patients with NSCLC. High serum miR-484 expression is associated with the diagnosis and prognosis of patients with non-small cell lung cancer

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MiR-106b-5p Promotes Proliferation and Inhibits Apoptosis by Regulating BTG3 in Non-Small Cell Lung Cancer

Cited by 49 publications

References 51 publications

miR-1323 Promotes Cell Migration in Lung Adenocarcinoma by Targeting Cbl-b and Is an Early Prognostic Biomarker

miR-1323 Promotes Cell Migration in Lung Adenocarcinoma by Targeting Cbl-b and Is an Early Prognostic Biomarker

miR‑106b‑5p promotes cell proliferation and cell cycle progression by directly targeting CDKN1A in osteosarcoma

High serum miR‑484 expression is associated with the diagnosis and prognosis of patients with non‑small cell lung cancer

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