miR-1323 Promotes Cell Migration in Lung Adenocarcinoma by Targeting Cbl-b and Is an Early Prognostic Biomarker

Abstract

Purpose: MicroRNAs are known to regulate cellular processes in non-small cell lung cancer (NSCLC) cells and predict prognosis. However, identification of specific microRNAs in NSCLC as potential therapeutic targets is controversial. We aim to determine the clinical significance of miR-1323 in the prognosis of patients with lung cancer and the potential mechanism. Patients and methods: A bioinformatics approach was used to screen the importance microRNA in NSCLC through the online GEO database (GSE42425). The relationship between expression level of miR-1323 and overall survival of lung cancer patients was analyzed. Additionally, an independent corhort including 53 NSCLC cases that underwent resection validated the connection between miR-1323 and LUAD patients' overall survival. Next, the function of miR-1323 was studied in vitro by transient transfection. A more in-depth mechanism was studied through luciferase reporter gene experiments. Results: High miR-1323 expression correlated with poor survival in NSCLC patients (P = 0.011), and in lung adenocarcinoma (LUAD) patients (P = 0.015) based on GEO database (GSE42425). In the independent cohort based on our hospital, high miR-1323 expression was associated with LUAD patients (P = 0.025). Moreover, transfection with mimics of miR-1323 showed an increased migratory capacity in LUAD A549 and HCC827 cells. In addition, E3 ubiquitin-protein ligase Casitas B-lineage Lymphoma-b (Cbl-b) was found to be the target genes of miR-1323 and significantly down regulated after mimics of miR-1323 transfection, and high Cbl-b expression predicted better prognosis in NSCLC and LUAD (P = 0.00072 and P = 0.02, respectively). Conclusion: The miR-1323 promoted LUAD migration through inhibiting Cbl-b expression. High miR-1323 expression predicted poor prognosis in LUAD patients.

MicroRNA‑1323 serves as a biomarker in gestational diabetes mellitus and aggravates high glucose‑induced inhibition of trophoblast cell viability by suppressing TP53INP1

“…Considering the increased expression of miR-1323 in patients with GDM, ROC curves were plotted based on the miR-1323 expression and revealed that miR-1323 may be a potential diagnostic biomarker for patients with GDM. Abnormal serum miR-1323 expression has been reported to be a candidate biomarker for numerous other diseases, including lung adenocarcinoma ( 30 ) and breast cancer ( 31 ). Therefore, we hypothesized that serum miR-1323 may be a novel and effective diagnostic biomarker for patients with GDM.…”

MicroRNA‑592 promotes cell proliferation, migration and invasion in colorectal cancer by directly targeting SPARC

“…MicroRNAs (miRNAs/miRs) are a class of small non-coding RNAs (18-25 nucleotides in length) that act as post-transcriptional regulators of gene expression by direct binding to the 3' untranslated regions (3'UTRs) of target mRNAs; this binding induces mRNA degradation and/or translational repression (4). miRNAs participate in a number of biological processes, such as cell cycle progression, proliferation, invasion, migration and cell metabolism (5)(6)(7)(8). The regulatory role of miR-592 is context-dependent.…”

miR-4284 Promotes Cell Proliferation, Migration, and Invasion in Non-Small Cell Lung Cancer Cells and is Associated with Postoperative Prognosis

“…15,16 In addition, it has been demonstrated that miRNAs play crucial roles in the pathogenesis and progression of cancers through functioning as oncogenic or tumor suppressor. 17 For instance, upregulation of miR-1323, 18 miR-212-5p, 19 and miR-671-3p 20 was found in NSCLC tissues and cells and participated in the proliferation, migration, invasion, and apoptosis as oncogenes. In addition, the downregulation of miR-144, 21 and miR-140-3p 22 functioned as a tumor suppressor in the development of NSCLC.…”